A5023463626- Nitric Oxide and Endothelin Effects
- Neuroscience of respiration and sleep
- High Altitude and Hypoxia
- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Hemoglobin structure and function
- Edible Oils Quality and Analysis
- Eicosanoids and Hypertension Pharmacology
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Cancer, Hypoxia, and Metabolism
- Heme Oxygenase-1 and Carbon Monoxide
- Fatty Acid Research and Health
- Mitochondrial Function and Pathology
- Neuropeptides and Animal Physiology
- Cardiac Ischemia and Reperfusion
- Phytochemicals and Antioxidant Activities
- Neurological Disease Mechanisms and Treatments
- Erythrocyte Function and Pathophysiology
- Biochemical effects in animals
- Antioxidant Activity and Oxidative Stress
- Alzheimer's disease research and treatments
- Diet and metabolism studies
- Neurogenesis and neuroplasticity mechanisms
- Adipose Tissue and Metabolism
- Advancements in Transdermal Drug Delivery
Universidad de Jaén
2012-2022
Universidad de Granada
2020
Ospedale di Civita Castellana
2005
University of Córdoba
2005
Oleuropein, the main phenolic compound of olive fruit, has important antioxidant properties that are responsible for some nutritional fruits and defence mechanism leaves. Polyphenol oxidase (PPO) activity changes during fruit ripening in many plants. We studied kinetics molecular PPO leaves (Olea europaea L.) cv. 'Picual' trees relationship between oleuropein concentration ripening. showed hyperbolic Significant increases specific activity, Vmax, Km catalytic efficiency occurred Based on...
Neuronal and inducible nitric oxide synthase (nNOS iNOS) nitrotyrosine immunoreactivities were localized semiquantitatively assessed in the cerebral cortex of aged rats by means light microscopic immunocytochemistry Western blotting, using a new series specific polyclonal antibodies. In strongly nNOS-immunoreactive multipolar neurons found layers II–VI young seen similar numbers, but showed varicose, vacuolated, fragmented processes, with an irregular outline loss spines. A large number more...
Stroke is one of the leading causes adult disability worldwide. After ischemic stroke, damaged tissue surrounding irreversibly core infarct, penumbra, still salvageable and therefore a target for acute therapeutic strategies. The Mediterranean diet (MD) has been shown to lower stroke risk. MD characterized by increased intake extra-virgin olive oil, which hydroxytyrosol (HT) foremost phenolic component. This study investigates effect an HT-enriched directly after on regaining motor cognitive...
Immune cells have been implicated in influencing stroke outcomes depending on their temporal dynamics, number, and spatial distribution after ischemia.Depending activation status, immune can detrimental beneficial properties tissue outcome stroke, highlighting the need to modulate inflammation towards restorative responses.Novel dietary therapies may promote modulation of pro-and anti-inflammatory cell functions.Among interventions inspired by Mediterranean diet, hydroxytyrosol (HT), main...
Objectives: Research has identified many factors associated with fibromyalgia (FM), but findings have been inconsistent. This study aimed to investigate changes in levels of nitric oxide (NO), inflammatory markers, lipid profile, and cortisol normal- overweight patients FM controls. Since most are overweight, we explored possible these markers according body mass index (BMI). Methods: preliminary was performed on serum samples women age-matched controls, grouped their BMI: 12 normal-weight...
The frequency of astrocytes, microglia plus oligodendrocytes, and pericytes displaying nuclei was analyzed quantified in 160-μm-wide strips the parietal cortex (Par1 region) from young aged Wistar rats. study performed on two groups rats 3–4 32–36 months. Quantifications glial cell types were made 1-μm-thick sections stained with toluidine blue. Ultrathin also to analyze ultrastructural features these cells during aging. Astrocytes increased number by about 20% 22%, respectively, age. These...
Background Different changes in neuronal and glial population of the aging brain have been described; however, degree extent these are controversial. This study evaluates quantitative cytomorphometric effects on populations parietal cortex rat. Methods The was performed two groups rats aged 4–6 30–32 months. Cortical volume, density, area, shapes soma nucleus were analyzed cortical layers I, II–IV, V, VI using serial sections stained with cresyl-fast-violet, morphometric techniques. Results...
Herein we report on the kinetic and protein expression of glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase, malic enzyme (ME) in liver trout ( Oncorhynchus mykiss) during a long-term starvation-refeeding cycle. Starvation significantly depressed activity these enzymes by almost 60%, without changing Michaelis constant. The time response to this nutritional stimulus increased with fish weight. sharp decline G6PDH ME activities was due specific protein-repression...
Abstract The quantitative morphological changes in neurons and glia during the aging process were analyzed different cortical layers, grouped as I, II–IV, V, VI, of frontal cortex rat. parameters volume, neuronal density, glial soma nucleus areas. No with age found volume density (with exception layer I), or area soma. However, older animals showed a 10 to 20% increase depending on studied. In addition, there was an age‐related decrease layers VI. These results support idea that undergoes...
Therapies against stroke can restore the blood supply but cannot prevent ischemic damage nor stimulate recovery of infarcted zone. The neuroglobin protein plays an important role in neuro-regeneration process after stroke; however, method for its effective systemic application has not been identified yet, as is unable to pass through blood-brain barrier. Previously, we developed different types sodium hyaluronate nanoparticles, which successfully cross barrier stroke. In this work, these...
Nitric oxide plays a critical role in many physiological and physiopathological processes the lung. Changes NO/NOS (Nitric Oxide/Nitric Oxide Synthase) system after hypoxia situations remain controversial this organ, so that aim of work is to perform complete study hypoxic lung different reoxygenation times ranging from 0 h 5 days posthypoxia. This novel follow-up carried out Wistar rats submitted for 30 min acute hypobaric hypoxia. We measured endothelial inducible NOS (eNOS, iNOS) mRNA...
The cerebellum is the neural structure with highest levels of nitric oxide, a neurotransmitter that has been proposed to play key role in brain aging, although knowledge concerning its contribution cerebellar senescence still unclear, due mainly absence integrative studies jointly evaluate main factors involved cell production and function. Consequently, present study, we investigate expression, location, activity oxide synthase isoenzymes; protein nitration; adult old rats.Our results show...
We have found conclusive evidence for inducible nitric oxide synthase (iNOS) activity in rainbow trout ( Oncorhynchus mykiss) tissue by means of biochemical, immunohistochemical, and immunoblotting analyses. This Ca 2+ -independent enzyme uses l-arginine to produce l-citrulline. It was significantly inhibited the analogs N ω -monomethyl-l-arginine G -nitro-l-arginine methyl ester. Kinetic analyses showed typical Michaelian behavior with no cooperative effects. The specific activities liver...
Hypoxia/reoxygenation (H/R) reportedly influences nitric oxide (NO) production and NO synthase (NOS) expression in the heart. Nonetheless, a number of works have shown controversial results regarding changes that cardiac NO/NOS system undergoes under such situations. Therefore, this study aims to clarify behaviour hypoxic heart by investigating seven different reoxygenation times. Wistar rats were submitted H/R (hypoxia for 30 min; 0, 2, 12, 24, 48, 72 h, 5 days) novel approach address...