A5100443257- Sexual function and dysfunction studies
- Sperm and Testicular Function
- Hormonal and reproductive studies
- Urological Disorders and Treatments
- Urologic and reproductive health conditions
- Urinary Bladder and Prostate Research
- Sexual Differentiation and Disorders
- Reproductive Biology and Fertility
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Urinary and Genital Oncology Studies
- Genital Health and Disease
- Sexuality, Behavior, and Technology
- Tissue Engineering and Regenerative Medicine
- Male Reproductive Health Studies
- Testicular diseases and treatments
- Mesenchymal stem cell research
- Healthcare and Venom Research
- Reproductive Health and Technologies
- Renal and related cancers
- Pelvic floor disorders treatments
- Extracellular vesicles in disease
- Pluripotent Stem Cells Research
- Renal cell carcinoma treatment
- Bladder and Urothelial Cancer Treatments
- Sarcoma Diagnosis and Treatment
Sun Yat-sen University
2016-2025
The First Affiliated Hospital, Sun Yat-sen University
2016-2025
University of Electronic Science and Technology of China
2024
Bridge University
2024
Nanjing University of Chinese Medicine
2024
University of Oxford
2023
John Wiley & Sons (United Kingdom)
2023
Hudson Institute
2023
John Wiley & Sons (United States)
2023
Central South University
2023
The ability to identify and isolate lineage-specific stem cells from adult tissues could facilitate cell replacement therapy. Leydig (LCs) are the primary source of androgen in mammalian testis, prospective identification (SLCs) may offer new opportunities for treating testosterone deficiency. Here, a transgenic mouse model expressing GFP driven by Nestin (Nes) promoter, we observed Nes-GFP+ located testicular interstitial compartment where SLCs normally reside. We showed that these...
Aim The aim of this study was to determine the possibility improving erectile dysfunction using cell therapy with either human urine-derived stem cells (USCs) or USCs genetically-modified FGF2 in a type 2 diabetic rat model. Methods Human were collected from 3 healthy donors. transfected (USCs-FGF2). Sixty-five SD male rats divided into five groups (G). A control group normal (G1, n = 10), and four other test rats: PBS as negative (G2, (G3, 15), lentivirus-FGF2 (G4, USCs-FGF2 (G5, 15)....
The aim of this study was to determine whether adipose derived stem cells (ADSCs) expressing vascular endothelial growth factor (VEGF) gene can improve function, recover the impaired VEGF signaling pathway and enhance smooth muscle contents in a rat diabetic erectile dysfunction (DED) model. DED rats were induced via intraperitoneal injection streptozotocin (40 mg/kg), then screened by apomorphine (100 µg/kg). Five groups used (n = 12/group)–Group 1 (G1): intracavernous lentivirus-VEGF; G2:...
The purpose of this study is to investigate the possible underlying pathogenesis erectile dysfunction(ED) in young men with low risk coronary heart disease and no well-known aetiology. To conduct study, 122 patients ED under age 40 were enrolled, along 33 age-matched normal control subjects. had significantly higher levels systolic blood pressure (SBP), total cholesterol triglyceride, high sensitivity C-reactive protein (hs-CRP), greater carotid intima-media thickness (CIMT) Framingham score...
Male late-onset hypogonadism is an age-related disease, the core mechanism of which dysfunction senescent Leydig cells.Recent studies have shown that elimination cells can restore proper homeostasis to aging tissue.In present study, we found fork head box O (FOXO) transcription factor FOXO4 was specially expressed in human and its translocation nucleus elderly related decreased testosterone synthesis.Using hydrogen peroxide-induced TM3 as vitro model, observed maintains viability suppresses...
Stem cell therapies represent a promising new frontier for the treatment of refractory diabetic erectile dysfunction (DED). The use stem cell-derived extracellular vesicles (EVs) is novel strategy cell-free therapy. We have reported that urine-derived cells (USCs) can improve DED; however, therapeutic effects EVs secreted by USCs (USC-EVs) remain unknown.To determine USC-EVs on DED in rat model.USC-EVs were isolated from conditioned medium ultracentrifugation. was induced male Sprague-Dawley...
Abstract STUDY QUESTION What is the molecular landscape underlying functional decline of human testicular ageing? SUMMARY ANSWER The present study provides a comprehensive single-cell transcriptomic atlas testes from young and old humans offers insights into mechanisms potential targets for ageing. WHAT IS KNOWN ALREADY Testicular ageing known to cause male age-related fertility hypogonadism. Dysfunction cells has been considered as key factor DESIGN, SIZE, DURATION Human biopsies were...
Abstract Stem Leydig cell (SLC) transplantation could provide a new strategy for treating the testosterone deficiency. Our previous study demonstrated that CD51 (also called integrin αv) might be putative surface marker SLCs, but physiological function and efficacy of CD51+ SLCs treatment remain unclear. Here, we explore potential therapeutic benefits whether these transplanted cells can regulated by hypothalamic-pituitary-gonadal (HPG) axis. were isolated from testes 12-weeks-old C57BL/6...
Abstract Endothelial cells (ECs) play a key role in revascularization within regenerating tissue. Stem are often used as an alternative cell source when ECs not available. Several types have been to give rise ECs, such umbilical cord vessels, or differentiated from somatic stem cells, embryonic, induced pluripotent cells. However, the latter carry potential risk of chronic immune rejection and oncogenesis. Autologous endothelial precursors ideal resource, but currently require invasive...
Abstract STUDY QUESTION Whether the testis-specific extracellular vesicle (EV) long noncoding RNAs (lncRNAs) in seminal plasma could be utilized to predict presence of testicular spermatozoa nonobstructive azoospermia (NOA) patients? SUMMARY ANSWER Our findings indicate that panel based on EV lncRNAs was a sensitive and specific method predicting may improve clinical decision-making NOA. WHAT IS KNOWN ALREADY The adoption sperm retrieval techniques, especially microdissection extraction...
Abstract Previous studies have demonstrated that rodent stem Leydig cell (SLC) transplantation can partially restore testosterone production in (LC)-disrupted or senescent animal models, which provides a promising approach for the treatment of hypogonadism. Here, we isolated human SLCs prospectively and explored potential therapeutic benefits SLC hypogonadism treatment. In adult testes, p75 neurotrophin receptor positive (p75 + ) cells expressed known marker nestin, but not LC lineage...
The aim of this study was to investigate whether intracavernous injection urine-derived stem cells (USCs) or USCs genetically modified with pigment epithelium-derived factor (PEDF) could protect the erectile function and cavernous structure in a bilateral nerve injury-induced dysfunction (CNIED) rat model. were cultured from urine six healthy male donors. Seventy-five rats randomly divided into five groups ( n = 15 per group): sham, (CN) crush injury (BCNI), USC, USCGFP+, USCGFP/PEDF+...
Nonobstructive azoospermia (NOA) is a severe form of male infertility, with limited effective treatments. Urine-derived stem cells (USCs) possess multipotent differentiation capacity and paracrine effects, participate in tissue repair regeneration. The aim this study to investigate whether the transplantation USCs or USC exosomes (USC-exos) could promote endogenous spermatogenesis restoration busulfan-induced NOA mice model. were cultured characterized by flow cytometry. High-density hollow...
Cavernosal endothelial dysfunction is one of the factors in developing diabetic erectile (DED), but mechanism cavernosal unclear. The present study aimed at determining contribution autophagy DED rats and explaining therapeutic effect urine-derived stem cells (USCs). After rat corpus vascular (CCECs) were isolated cultured vitro, CCECs treated with advanced glycation end products (AGEs) to mimic situation. Autophagy flux, proliferation, apoptosis determined by mRFP-GFP-LC3 adenovirus...
Background: Testicular aging is associated with diminished fertility and certain age-related ailments, effective therapeutic interventions remain elusive. Here, we probed the efficacy of exosomes derived from human umbilical cord mesenchymal stem cells (hUMSC-Exos) in counteracting testicular aging. Methods: We employed a model 22-month-old mice administered intratesticular injections hUMSC-Exos. Comprehensive analyses encompassing immunohistological, transcriptomic, physiological...