A5089914375- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Supramolecular Self-Assembly in Materials
- Chemical Synthesis and Analysis
- Vibrio bacteria research studies
- Polydiacetylene-based materials and applications
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Aquaculture disease management and microbiota
- Peptidase Inhibition and Analysis
- RNA and protein synthesis mechanisms
- Crystallography and molecular interactions
- Zeolite Catalysis and Synthesis
- Advanced Chemical Physics Studies
- Escherichia coli research studies
- Salmonella and Campylobacter epidemiology
- Rice Cultivation and Yield Improvement
- Advanced NMR Techniques and Applications
- Spectroscopy and Quantum Chemical Studies
- Extracellular vesicles in disease
- Magnetic Field Sensors Techniques
- Ferroptosis and cancer prognosis
- SARS-CoV-2 and COVID-19 Research
- Plant Growth Enhancement Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
Artemis Hospitals
2024
Jamia Hamdard
2016-2023
Institute of Molecular Medicine
2011-2023
Adamas University
2022-2023
Government of West Bengal
2020
Bidhan Chandra Krishi Viswavidyalaya
2017-2020
National Taiwan University of Science and Technology
2012-2016
Indian Institute of Chemical Biology
1995-2011
National Cancer Institute
2001-2011
National Institutes of Health
2001-2011
Most HIV transmission occurs on the mucosal surfaces of gastrointestinal and cervicovaginal tracts, both which are normally coated by a biofilm nonpathogenic commensal bacteria. We propose to genetically engineer such naturally occurring bacteria protect against infection secreting antiviral peptides. Here we describe development characterization Nissle 1917, highly colonizing probiotic strain Escherichia coli , HIV-gp41-hemolysin A hybrid peptides that block fusion entry into target cells....
Bartonella bacilliformis, which causes the human diseases Oroya fever and verruga peruana, binds to erythrocytes in vitro produces substantial long-lasting deformations erythrocyte membranes, including cone-shaped depressions, trenches, deep invaginations. The deforming force is probably provided by polar flagella of these highly motile bacteria. Deep invaginations containing bacteria are commonly seen, membrane fusion at necks leads formation intracellular vacuoles Fluorescent compounds...
Bacterial nucleoid organization is believed to have minimal influence on the global transcription program. Using an altered bacterial histone-like protein, HUα, we show that reorganization of configuration can dynamically modulate cellular pattern. The mutant protein transformed loosely packed into a densely condensed structure. compaction, coupled with increased DNA supercoiling, generated radical changes in morphology, physiology, and metabolism wild-type K-12 Escherichia coli . Many...
Abstract Leprosy, a progressive, mutilating and highly stigmatized disease caused by Mycobacterium leprae (ML), continues to prevail in the developing world. This is due absence of rapid, specific sensitive diagnostic tools for its early detection since gets notified only with advent physical scarring patients. study reports development Loop-mediated isothermal amplification (LAMP) technique fast, 16S rRNA gene ML DNA leprosy resource-limited areas. Various parameters were optimized obtain...
The aim of this study was to evaluate and characterize the antileishmanial efficacy fucoidan, a polyanionic sulphated polysaccharide from brown algae, in experimental infections BALB/c mice with antimony-susceptible (AG83) -resistant (GE18ER) Leishmania donovani. effect fucoidan assessed against intracellular parasites cultured macrophages suppressing splenic liver parasite burdens mouse model visceral leishmaniasis by microscopic evaluation surviving amastigotes stained Giemsa. To type...
Oxaliplatin (Oxa) treatment to SH-SY5Y human neuroblastoma cells has been shown by previous studies induce oxidative stress, which in turn modulates intracellular signaling cascades resulting cell death. While this phenomenon of Oxa-induced neurotoxicity is known, the underlying mechanisms involved death cascade must be clarified. Moreover, there still little known regarding roles neuronal mitochondria and cytosolic compartments mediating neurotoxicity. With a better grasp driving...
The HUα(E38K, V42L) mutant of the bacterial histone-like protein HU causes a major change in transcription profile commensal organism Escherichia coli K-12 (Kar S, Edgar R, Adhya Proc. Natl. Acad. Sci. U. S. A. 102:16397-16402, 2005). Among upregulated genes are several related to pathogenic interactions with mammalian cells, as evidenced by expression curli fibers, Ivy, and hemolysin E. When K-12/ was added Int-407 there host cell invasion, phagosomal disruption, intracellular replication....
In Gal repressosome assembly, a DNA loop is formed by the interaction of two GalR, bound to distal operators, and binding histone-like protein, HU, an architecturally critical position on facilitate GalR-GalR interaction. We show that GalR piggybacks HU through specific GalR-HU This first example making contact with another protein. The results in cooperative proteins may be transient absent final structure. A sequence-independent DNA-binding protein being recruited architectural site...
Gallbladder cancer (GBC) is an aggressive malignancy of the gastrointestinal tract with a poor prognosis. It important to understand molecular processes associated pathogenesis early stage GBC and identify proteins useful for diagnostic therapeutic strategies. Here, we have carried out iTRAQ-based quantitative proteomic analysis tumor tissues from cases (stage I, n=7 II, n=5) non-tumor controls (n=6) gallstone disease (GSD). We identified 357 differentially expressed (DEPs) based on ≥ 2...
Commensal Escherichia coli has been identified as a major protagonist of microbe-induced colorectal oncogenesis. Its tumour-promoting attribute is linked to the expression DNA-damaging genotoxins. Using constitutively invasive variant non-pathogenic E. coli, we demonstrate that chronic presence internalized leads enhanced oncogenicity in colon cancer cells. Instead genomic damage, tumorigenic effect mediated through an expansion stem cell (CSC) population, likely dedifferentiation...
We studied the restriction fragment length polymorphism of rRNA gene and CTX genetic element in Vibrio cholerae O139 Bengal, which resurged Calcutta September 1996 after a gap 32 months. While strains from this resurgence were indistinguishable earlier by ribotyping, structure present current was found to be unconventional.
Summary Sixty-nine strains of Vibrio cholerae 01 isolated at different times were analysed to investigate if there any differences among the before, during and after advent O139 serogroup. Of 69 examined, 68 belonged Ogawa serotype while one Inaba serotype. With exception strain all other V. eltor biotype. A single O1 before emergence serogroup could not be classified as either or classical biotype because it was resistant both specific bacteriophages. Marked variations in susceptibility...
Abstract Background Early diagnosis is important for the timely treatment of gallbladder carcinoma (GBC) patients and may lead to increased survival outcomes. Here, we have applied serological proteome analysis (SERPA), an immunoproteomics approach, detection ‘tumor-associated antigens (TAAs) that elicit humoral response’ in early stage GBC patients. Methods Total protein from pooled tumor tissue ( n = 7) was resolved by two-dimensional gel electrophoresis (2-DE) followed immunoblotting...
Abstract Gallbladder carcinoma (GBC) is a major cancer of the gastrointestinal tract with poor prognosis. Reliable and affordable biomarker-based assays high sensitivity specificity for detection this are clinical need. With aim studying potential plasma-derived extracellular vesicles (EVs), we carried out quantitative proteomic analysis EV proteins, using three types controls various stages disease, which led to identification 86 proteins altered abundance. These include 29 unique early...
An unusual filamentous bacteriophage, VSK, containing single-stranded, circular DNA as its genome was isolated from Vibrio cholerae 0139 strains P07 and B04. Unlike other single-stranded phages, VSK can integrate into the chromosome of host enter a lysogenic state. The double-stranded replicative form (RF) phage isolated. A restriction map RF constructed using HaeII, AvaII, ClaI XbaI. By Southern blot analysis chromosomal lysogen labeled probe, attachment site (attP) on viral also identified.
DNA transaction reactions require formation of nucleoprotein complexes that involve multifaceted DNA-protein and protein-protein interactions. Genetic biochemical studies suggested the higher order Gal repressosome structure, which governs transcription two tandem galpromoters in Escherichia coli, involves sequence-specific binding GalR repressor dimers to operators, O(E) O(I), located 113 bp apart, sequence-nonspecific protein HU, interaction HU with an architecturally critical site between...