A5102019255- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Salmonella and Campylobacter epidemiology
- Agronomic Practices and Intercropping Systems
- Biochemical and Molecular Research
- Tuberculosis Research and Epidemiology
- Crop Yield and Soil Fertility
- Mycobacterium research and diagnosis
- Composting and Vermicomposting Techniques
- Rice Cultivation and Yield Improvement
- Legume Nitrogen Fixing Symbiosis
- Agricultural Science and Fertilization
- Soil Carbon and Nitrogen Dynamics
- Bacteriophages and microbial interactions
- Soybean genetics and cultivation
- Synthesis and bioactivity of alkaloids
- Antibiotic Resistance in Bacteria
- Plant tissue culture and regeneration
- Pharmaceutical and Antibiotic Environmental Impacts
- Identification and Quantification in Food
- Escherichia coli research studies
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
Council of Scientific and Industrial Research
2017-2019
Institute of Microbial Technology
1993-2019
Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya
2000-2012
National Institute of Cholera and Enteric Diseases
1997
ABSTRACT There was an inexplicable upsurge in the incidence of non-O1, non-O139 Vibrio cholerae among hospitalized patients admitted to Infectious Diseases Hospital, Calcutta, India, between February and March 1996. Of 18 strains V. isolated during this period, 15 belonged serogroups (4 O144, 3 O11, 1 each O6, O8, O12, O19, O39, O58, 2 could not be typed), O139 serogroup, O1 serogroup. Cell-free culture supernatants 13 representative evoked a distinct cytotoxic effect on CHO HeLa cells,...
Sixty-one clinical strains of Vibrio cholerae Ol El Tor isolated in Calcutta before, during, and after the V. O139 Bengal outbreak were examined to see if O1 post-O139 period different from those existence before. Comparison restriction fragment length polymorphism rRNA genes (ribotyping) CTX genetic element revealed that all "before" except 1 belonged a single known ribotype, whereas "after" 2 hitherto undescribed ribotype. Also, 23 25 harbored two or more copies tandem also "free" RS1 away...
We studied the restriction fragment length polymorphism of rRNA gene and CTX genetic element in Vibrio cholerae O139 Bengal, which resurged Calcutta September 1996 after a gap 32 months. While strains from this resurgence were indistinguishable earlier by ribotyping, structure present current was found to be unconventional.
Natural evolution in microbes exposed to antibiotics causes inevitable selection of resistant mutants. This turns out be a vicious cycle which requires the continuous discovery new and effective antibiotics. For last six decades, we have been relying on semisynthetic derivatives natural products discovered "Golden Era" from microbes, especially Streptomyces sp. Low success rates rational drug-design sparked resurgence invention novel or scaffolds untapped uncommon microbial niches....
ABSTRACT We present molecular evidence that a distinct genotype of Vibrio cholerae O1 which appeared in Calcutta, India, September 1993 and is characterized by unique ribotype not found the standardized ribotyping scheme V. shows specific pulsed-field gel electrophoresis profile may have spread to west African country Guinea-Bissau where it was responsible for an epidemic cholera began October 1994 continued into 1996.
The Mycobacterium tuberculosis exported repetitive protein (RvErp) is a crucial virulence-associated factor as determined by its role in the survival and multiplication of mycobacteria cultured macrophages vivo Although attempts have been made to understand function Erp protein, exact pathogenesis still elusive. One way determine this searching for novel interactions RvErp. Using yeast two-hybrid assay, an adenylyl cyclase (AC), Rv2212, was found interact with interaction between RvErp...
Surmmary Four lines of evidence suggest that the recent outbreak strains Vibrio cholerae O139 could have emerged from serogroup O1 typified by isolates M01 and M0477 described in this paper, which are neither truly classical nor E1 Tor their biotype attributes. Firstly, like all isolates, these strains, isolated Madras during before outbreak, were resistant not only to polymyxin B but also biotype-specific choleraphages, i.e. phage Φ149 phages e4 e5. Secondly, restriction fragment pattern...
Eis (Enhanced Intracellular Survival) is an important aminoglycoside N-acetyltransferase enzyme contributing to kanamycin resistance in Mtb clinical isolates. proteins from M. tuberculosis (RvEis) and smegmatis (MsEis) have 58% identical 69% similar amino acid sequences acetylate aminoglycosides at multiple amines. Both the are hexameric composed of two symmetric trimers. RvEis has remarkable structural stability heat-stable acetyltransferase activity. Although structure biochemical...