A5010395693- Asthma and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Respiratory Support and Mechanisms
- Neonatal Respiratory Health Research
- Inhalation and Respiratory Drug Delivery
- Pediatric health and respiratory diseases
- Respiratory viral infections research
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Nuclear Structure and Function
- Respiratory and Cough-Related Research
- Tracheal and airway disorders
- Metabolomics and Mass Spectrometry Studies
- Chemokine receptors and signaling
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Axon Guidance and Neuronal Signaling
- Eicosanoids and Hypertension Pharmacology
- Cellular Mechanics and Interactions
- Lung Cancer Diagnosis and Treatment
- Microtubule and mitosis dynamics
- Skin and Cellular Biology Research
- Occupational and environmental lung diseases
- Genetic Neurodegenerative Diseases
- Nutrition and Health in Aging
Centre de Recherche Cardio-Thoracique de Bordeaux
2016-2025
Inserm
2016-2025
Université de Bordeaux
2013-2024
Bordeaux Population Health
2022-2024
Institut Universitaire de France
2024
Centre National de la Recherche Scientifique
2011-2024
Institut Interdisciplinaire de Neuroscience
2012-2023
Centre Hospitalier Universitaire de Bordeaux
2022-2023
Imaging Center
2023
Institut Élie Cartan de Lorraine
2023
Control of cell polarity is crucial during tissue morphogenesis and renewal, depends on spatial cues provided by the extracellular environment. Using micropatterned substrates to impose reproducible cell-cell interactions, we show that in absence other polarizing cues, contacts are main regulator nucleus centrosome positioning, intracellular polarized organization. In a variety types, including astrocytes, epithelial cells, endothelial calcium-dependent cadherin-mediated interactions induce...
The localization of the nucleus is precisely regulated, and defects in nuclear positioning are observed diseases such as lissencephaly, cerebellar ataxia dysplasia. We show here that cytoplasmic intermediate filaments essential players actin-dependent nucleus. actin retrograde flow relayed by a accumulate asymmetrically around envelope. Perturbations filament network alter both migrating immobile astrocytes. This function might be crucial for regulating cell motility, particular tumor cells...
Rationale: Severe asthma is a major public health issue throughout the world. Increased bronchial smooth muscle (BSM) mass, characteristic feature of airway remodeling in severe asthma, associated with resistance to high-intensity treatment and poor prognosis. In vitro, Ca2+-channel blocker gallopamil decreased proliferation BSM cells from patients asthma.Objectives: We conducted double-blind, randomized, placebo-controlled study evaluate effect on asthma.Methods: Subjects received either (n...
Background Bronchial smooth muscle (BSM) remodelling in asthma is related to an increased mitochondrial biogenesis and enhanced BSM cell proliferation asthma. Since mitochondria produce the highest levels of cellular energy fatty acid β-oxidation most powerful way ATP, we hypothesised that, asthmatic cells, energetic metabolism shifted towards acids. Objectives We aimed characterise both vitro ex vivo identify a novel target for reducing proliferation. Methods 21 31 non-asthmatic patients...
Asthma is a frequent airway disease, and asthma control determinants have been associated with indoor allergen sensitization. The most allergens are house dust mites (HDM), which act in vivo on the bronchial epithelial layer. Severe has also remodeling more specifically increased mass of smooth muscle (BSM). However, relationship between HDM stimulation layer BSM unknown.To evaluate whether induces cell proliferation subjects severe asthma.A total 22 27 no were recruited. We developed an...
Rationale: Increased bronchial smooth muscle (BSM) mass is a key feature of airway remodeling that classically distinguishes severe from nonsevere asthma. Proliferation BSM cells involves specific mitochondria-dependent pathway in individuals with However, and mitochondrial biogenesis have not been examined asthma.Objectives: We aimed to assess whether an increase was also implicated asthma its relationship mitochondria clinical outcomes.Methods: enrolled 34 never-smoker subjects In...
The remodelling mechanism and cellular players causing persistent airflow limitation in COPD remain largely elusive. We have recently demonstrated that circulating fibrocytes, a rare population of fibroblast-like cells produced by the bone marrow stroma, are increased patients during an exacerbation. aimed to quantify fibrocyte density situ bronchial specimens from both control subjects patients, define associations with relevant clinical, functional computed tomography (CT) parameters,...
Chronic obstructive pulmonary disease (COPD) is a prevalent respiratory lacking effective treatment. Focusing on early COPD should help to discover modifying therapies. We examined the role of CXCL12/CXCR4 axis in using human samples and murine models. Blood lung tissues from both individuals with controls were analyzed for CXCL12 CXCR4 levels. To generate an early-like model, 10-week-old male C57BL/6J mice exposed cigarette smoke (CS) 10 weeks intranasal instillations...
Rationale: Three-dimensional (3D) culture models such as alveolosphere, provide a unique tool to study emphysema mechanisms. Reducing the heterogeneity of alveolospheres which are currently mostly grown in Matrigel remains challenging. Objectives: To develop tunable and reproducible 3D-alveolosphere model exclusively from human primary type II alveolar epithelial cells (AEC2) for modeling understanding emphysema. Methods: AEC2 (HTII-280+) were isolated 52 smoker non-smoker lung samples...
Animal models and, in particular, mice models, are important tools to investigate the pathogenesis of respiratory diseases and test potential new therapeutic drugs. Lung function measurement is a key step such investigation. In mice, it usually performed using forced oscillation technique (FOT), negative pressure-driven expiratory (NPFE) pressure-volume (PV) curve maneuvers. However, these techniques require tracheostomy, which therefore only allows end-point measurements. Orotracheal...
Rhinovirus (RV) infection of the bronchial epithelium is implicated in vast majority severe asthma exacerbations. Interestingly, susceptibility to RV increased persons with asthma. Bronchial smooth muscle (BSM) remodeling an important feature pathophysiology, and its reduction using thermoplasty has been associated a significant decrease exacerbation rate. We hypothesized that asthmatic BSM can play role epithelium. Using original co-culture model between cells, we show cells increase...
Background Airflow limitation is the hallmark of obstructive pulmonary diseases, with distal airways representing a major site obstruction. Although numerous in vitro models bronchi already exist, there currently no culture system for diseases that reproduces architecture and function small airways. Here, we aimed to engineer model overcome limitations current systems. Methods We developed so-called bronchioid by encapsulating human bronchial adult stem cells derived from clinical samples...
Axon pathfinding is controlled by guidance cues that elicit specific attractive or repulsive responses in growth cones. It has now become clear some such as Netrin-1 can trigger either attraction repulsion a context-dependent manner. In particular, it was recently found the repellent Slit1 enables response of rostral thalamic axons to Netrin-1. This finding raised intriguing possibility and Slit1, two essential cues, may act more generally an unexpected combinatorial manner orient axonal...
BackgroundPatients with severe asthma show an increase in both exacerbation frequency and bronchial smooth muscle (BSM) mass. Rhinovirus (RV) infection of the epithelium (BE) is main trigger exacerbations. Histological analysis biopsies shows that a close connection between BE hypertrophic BSM criterion for severity asthma.ObjectiveWe hypothesized RV specifically increases BSM-cell migration from patients asthma.MethodsSerum samples, biopsies, or cells were obtained 86 31 subjects without...