A5056715361- Cannabis and Cannabinoid Research
- Neuroscience and Neuropharmacology Research
- Regulation of Appetite and Obesity
- HIV Research and Treatment
- Adipose Tissue and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Pancreatic function and diabetes
- Neurogenesis and neuroplasticity mechanisms
- HIV/AIDS drug development and treatment
- Parkinson's Disease Mechanisms and Treatments
- Diet and metabolism studies
- Genetics and Neurodevelopmental Disorders
- Alzheimer's disease research and treatments
- Herpesvirus Infections and Treatments
- Memory and Neural Mechanisms
- Neurological disorders and treatments
- Diet, Metabolism, and Disease
- Multiple Sclerosis Research Studies
- HIV-related health complications and treatments
- Asthma and respiratory diseases
- Neurological diseases and metabolism
- Cancer, Stress, Anesthesia, and Immune Response
- Dietary Effects on Health
- Ubiquitin and proteasome pathways
- Pain Mechanisms and Treatments
Université de Bordeaux
2015-2025
Inserm
2016-2025
Neurocentre Magendie
2016-2025
Centre de Recherche Cardio-Thoracique de Bordeaux
2022
Hôpital Xavier Arnozan
2022
Institut Européen de Chimie et Biologie
2007-2008
Centre National de la Recherche Scientifique
1996-2005
Université Paris Cité
2005
Institut Cochin
1999-2004
Institut de Myologie
2003
Cell proliferation and neuroinflammation in the adult hypothalamus may contribute to pathogenesis of obesity. We tested whether intertwining these two processes plays a role metabolic changes caused by 3 weeks high–saturated fat diet (HFD) consumption. Compared with chow-fed mice, HFD-fed mice had rapid increase body weight mass specifically showed an increased number microglia arcuate nucleus (ARC) hypothalamus. Microglia expansion required adequate presence fats carbohydrates because...
Background The discovery of the endocannabinoid system and its role in regulation energy balance has significantly advanced our understanding physiopathological mechanisms leading to obesity type 2 diabetes. New knowledge on this humans been acquired by measuring blood endocannabinoids. Here we explored endocannabinoids related N-acylethanolamines saliva verified their changes relation body weight status response a meal or loss. Methodology/Principal Findings Fasting plasma salivary were...
Surgery and cisplatin‐based treatment of hepatoblastoma (HB) currently guarantee the survival 70%‐80% patients. However, some important challenges remain in diagnosing high‐risk tumors identifying relevant targetable pathways offering new therapeutic avenues. Previously, two molecular subclasses HB have been described, C1 C2, with C2 being subgroup poorest prognosis, a more advanced tumor stage, worst overall rate. An associated 16‐gene signature to discriminate tumoral subgroups was...
Complex interactions between periphery and the brain regulate food intake in mammals. Cannabinoid type-1 (CB 1 ) receptor antagonists are potent hypophagic agents, but sites where this acute action is exerted underlying mechanisms not fully elucidated. To dissect effect of CB blockade, we combined injection antagonist rimonabant with use conditional -knockout mice, as well pharmacological modulation different central peripheral circuits. Fasting/refeeding experiments revealed that signaling...
Multiple system atrophy (MSA) is a sporadic orphan neurodegenerative disorder. No treatment currently available to slow down the aggressive process, and patients die within few years after disease onset. The cytopathological hallmark of MSA accumulation alpha-synuclein (α-syn) aggregates in affected oligodendrocytes. Several studies point α-syn oligomerization aggregation as mediator neurotoxicity synucleinopathies including MSA. C-terminal truncation by inflammatory protease caspase-1 has...
Hepatocellular adenomas (HCAs) are rare benign tumors divided into three main subgroups defined by pathomolecular features, HNF1A (H-HCA), mutated β-catenin (b-HCA), and inflammatory (IHCA). In the case of unclassified HCAs (UHCAs), which currently identified default, a high risk bleeding remains clinical issue. The objective this study was to explore UHCA proteome with aim identify specific biomarkers. Following dissection tumoral (T) nontumoral (NT) tissue on formalin-fixed,...
Type-1 cannabinoid (CB1) and leptin (ObR) receptors regulate metabolic astroglial functions, but the potential links between two systems in astrocytes were not investigated so far. Genetic pharmacological manipulations of CB1 receptor expression activity cultured cortical hypothalamic demonstrated that signaling controls levels ObR expression. Lack also markedly impaired leptin-mediated activation signal transducers activators transcription 3 5 (STAT3 STAT5) astrocytes. In particular,...
Metabolic flexibility allows rapid adaptation to dietary change, however, little is known about the CNS mechanisms regulating this process. Neurons in hypothalamic ventromedial nucleus (VMN) participate energy balance and are target of metabolically relevant hormone leptin. Cannabinoid type-1 (CB1) receptors expressed VMN neurons, but specific contribution endocannabinoid signaling neuronal population regulation unknown. Here we demonstrate that CB1 regulate metabolic actions In chow-fed...
Memory impairment is one of the disabling manifestations multiple sclerosis (MS) possibly present from early stages disease and for which there no specific treatment. Hippocampal synaptic dysfunction dendritic loss, associated with microglial activation, can underlie memory deficits, yet molecular mechanisms driving such hippocampal neurodegeneration need to be elucidated. In early-stage experimental autoimmune encephalomyelitis (EAE) female mice, we assessed expression level molecules...
Background Leucine supplementation might have therapeutic potential in preventing diet-induced obesity and improving insulin sensitivity. However, the underlying mechanisms are at present unclear. Additionally, it is unclear whether leucine be equally efficacious once has developed. Methodology/Principal Findings Male C57BL/6J mice were fed chow or a high-fat diet (HFD), supplemented not with for 17 weeks. Another group of HFD-fed (HFD-pairfat group) was food restricted order to reach an...
Adult neurogenesis occurs in the dentate gyrus (DG) of hippocampus, which is a key structure learning and memory. Adult-generated granule cells have been shown to play role spatial memory processes such as acquisition or retrieval, particular during an immature stage when they exhibit period increased plasticity. Here, we demonstrate that mature neurons born DG adult rats are similarly activated processes. By imaging activation these two different neuron generations same rat by using...
Machine learning–based approach unravels distinct pathological signatures induced by patient-derived α-synuclein seeds in monkeys.
Abstract Strong breakthrough pain is one of the most disabling symptoms cancer since it affects up to 90% patients and often refractory treatments. Alteration in gene expression a known mechanism which microRNAs (miRNAs), class non-coding regulatory RNAs, play crucial role. Here, mouse model pain, we show that miR-124 down-regulated spinal cord, first relay signal brain. Using vitro vivo approaches, demonstrate an endogenous specific inhibitor synaptopodin (Synpo), key protein for synaptic...
The paraventricular nucleus of the hypothalamus (PVN) regulates energy balance by modulating not only food intake, but also expenditure (EE) and brown adipose tissue thermogenesis. To test hypothesis that cannabinoid type 1 (CB1) receptor in PVN neurons might control these processes, we used Cre/loxP system to delete CB1 from single-minded (Sim1) neurons, which account for majority neurons. On standard chow, mice lacking Sim1 (Sim1-CB1-knockout [KO]) had body weight, adiposity, glucose...
Until recently, 10% of hepatocellular adenomas (HCAs) remained unclassified (UHCA). Among the UHCAs, sonic hedgehog HCA (shHCA) was defined by focal deletions that fuse promoter Inhibin beta E chain with GLI1. Prostaglandin D2 synthase proposed as immunomarker. In parallel, our previous work using proteomic analysis showed most UHCAs constitute a homogeneous subtype associated overexpression argininosuccinate (ASS1). To clarify use ASS1 in classification and avoid misinterpretations...
Spinal reactive astrocytes and microglia are known to participate the initiation maintenance of neuropathic pain. However, whether in thalamic nuclei that process sensory-discriminative aspects pain play a role behavior remains poorly investigated. Therefore, present study evaluated presence glia (hypertrophy, increased number upregulation glial markers) ventral posterolateral nucleus (VPL) correlates with symptoms, 14 28 days after unilateral L5/L6 spinal nerve ligation (SNL)...
Abstract The hypothalamus contains a remarkable diversity of neurons that orchestrate behavioural and metabolic outputs in highly plastic manner. Neuronal is key to enabling hypothalamic functions and, according the neuroscience dogma, it predetermined during embryonic life. Here, by combining lineage tracing pro-opiomelanocortin (Pomc) with single-cell profiling approaches adult male mice, we uncovered subpopulations ‘Ghost’ endowed atypical molecular functional identity. Compared...
Obesity-induced biological changes often persist after weight loss and are difficult to reverse, a phenomenon known as ‘obesogenic memory’. This enduring effect is associated with metabolic inflammation, particularly in adipose tissue. In this study, we characterise mouse model of obesogenic memory evaluate the efficacy unimolecular conjugate GLP-1/Dexa, which selectively safely delivers anti-inflammatory drug dexamethasone GLP-1 receptor (GLP-1R)-expressing cells. We document that precision...
Strains of the feline immunodeficiency virus (FIV) presently under investigation exhibit distinct patterns in vitro tropism. In particular, adaptation FIV for propagation Crandell kidney (CrFK) cells results selection strains capable forming syncytia with cell lines diverse species origin. The infection CrFK by CrFK-adapted appears to require chemokine receptor CXCR4 and is inhibited its natural ligand, stromal cell-derived factor 1alpha (SDF-1alpha). Here we found that inhibitors...
Objective High‐protein diets favor weight loss and its maintenance. Whether these effects might be recapitulated by certain amino acids is unknown. Therefore, the impact of leucine supplementation on energy balance associated metabolic changes in diet‐induced obese (DIO) mice during after was investigated. Methods DIO C57BL/6J were fed a normocaloric diet to induce while receiving or not acid drinking water. Body weight, food intake, body composition, expenditure, glucose tolerance, insulin,...
Rhinovirus (RV) infection of the bronchial epithelium is implicated in vast majority severe asthma exacerbations. Interestingly, susceptibility to RV increased persons with asthma. Bronchial smooth muscle (BSM) remodeling an important feature pathophysiology, and its reduction using thermoplasty has been associated a significant decrease exacerbation rate. We hypothesized that asthmatic BSM can play role epithelium. Using original co-culture model between cells, we show cells increase...