A5006731614- Regulation of Appetite and Obesity
- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Biochemical Analysis and Sensing Techniques
- Pancreatic function and diabetes
- Receptor Mechanisms and Signaling
- Diet, Metabolism, and Disease
- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Sleep and Wakefulness Research
- Neuroscience of respiration and sleep
- Biochemical effects in animals
- Connexins and lens biology
- Diabetes Management and Research
- Nitric Oxide and Endothelin Effects
- Tryptophan and brain disorders
- Metabolism, Diabetes, and Cancer
- Stress Responses and Cortisol
- Olfactory and Sensory Function Studies
- Cannabis and Cannabinoid Research
- Heart Rate Variability and Autonomic Control
- Photoreceptor and optogenetics research
- Dietary Effects on Health
- Nicotinic Acetylcholine Receptors Study
- Digestive system and related health
NutriNeuro
2017-2024
Université de Bordeaux
2018-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Institut Polytechnique de Bordeaux
2018-2024
Centre des Sciences du Goût et de l'Alimentation
2012-2023
Université de Bourgogne
2012-2023
Centre National de la Recherche Scientifique
2012-2023
Université Bourgogne Franche-Comté
2016-2023
Medical Research Network
2022
Institut Agro Dijon
2013-2017
Four types of responses to glucose changes have been described in the arcuate nucleus (ARC): excitation or inhibition by low concentrations <5 mmol/l (glucose-excited and -inhibited neurons) high >5 (high glucose-excited neurons). However, ability same ARC neuron detect has never investigated. Moreover, mechanism involved mediating sensitivity glucose-inhibited neurons neurotransmitter identity (neuropeptide Y [NPY] pro-opio melanocortin [POMC]) glucosensing remained controversial. Using...
Background and Purpose The link between type 2 diabetes mellitus (T2DM) depression is bidirectional. However, the possibility that metabolic disorders may elicit anxiogenic‐like/depressive‐like symptoms or alter efficacy of antidepressant drugs remains poorly documented. This study explored influence T2DM on emotionality proposed a therapeutic strategy might be used in depressed diabetic patients. Experimental Approach Mice were fed high‐fat diet (HFD) subjected to full comprehensive...
Epidemiological studies indicate that insulin resistance (IR), a hallmark of type 2 diabetes, is associated with an increased risk major depression. Here, we demonstrated male mice fed high-fat diet (HFD) exhibited peripheral metabolic impairments reminiscent IR accompanied by elevated circulating levels branched-chain amino acids (BCAAs), whereas both parameters were normalized chronic treatment metformin (Met). Given the role BCAAs in regulation tryptophan influx into brain, then explored...
Glucagon-like peptide 1 (GLP-1) stimulates insulin secretion and holds significant pharmacological potential. Nevertheless, the regulation of energy homeostasis by centrally-produced GLP-1 remains partially understood. Preproglucagon cells, known to release GLP-1, are found in olfactory bulb (OB). We show that activating receptors (GLP-1R) OB response oral glucose lean diet-induced obese male mice. This is associated with reduced noradrenaline content pancreas blocked an α2-adrenergic...
The ability for the brain to sense peripheral fuel availability is mainly accomplished within hypothalamus, which detects ongoing systemic nutrients and adjusts food intake metabolism as needed. Here, we hypothesized that mitochondrial reactive oxygen species (ROS) could trigger sensing of hypothalamus. For this purpose, induced acute hypertriglyceridemia in rats examined function mitochondria Hypertriglyceridemia led a rapid increase respiration ventral hypothalamus together with transient...
A physiological adaptation to a sugar-rich meal is achieved by increased sugar uptake match dietary load, resulting from rapid transient translocation of the fructose/glucose GLUT2 transporter brush border membrane (BBM) enterocytes. The aim this study was define contributors and mechanisms controlling intestinal absorption, focusing on action insulin contribution GLUT2-mediated transport.The studies were performed in human enterocytic colon carcinoma TC7 subclone (Caco-2/TC7) cells vivo...
Nitrates such as nitroglycerin (GTN) and nitric oxide donors S-nitrosothiols are clinically vasoactive through stimulation of soluble guanylyl cyclase (sGC), which produces the second messenger cGMP. Development nitrate tolerance, after exposure to GTN for several hours, is a major drawback widely used cardiovascular therapy. We recently showed that causes S-nitrosylation sGC, directly desensitizes sGC by oxide. tested hypothesis desensitization mechanism tolerance. Our results established...
OBJECTIVE The response of ventromedial hypothalamic (VMH) glucose-inhibited neurons to decreased glucose is impaired under conditions where the counterregulatory (CRR) hypoglycemia (e.g., recurrent hypoglycemia). This suggests a role for in CRR. We recently showed that increases nitric oxide (NO) production cultured VMH neurons. These vitro data led us hypothesize NO release from critical RESEARCH DESIGN AND METHODS CRR was evaluated rats and mice acute insulin-induced hypoglycemic clamps...
The C57Bl/6J (Bl/6J) mouse is the most widely used strain in metabolic research. This carries a mutation nicotinamide nucleotide transhydrogenase (Nnt), mitochondrial enzyme involved NADPH production, which has been suggested to lead glucose intolerance and beta-cell dysfunction. However, recent reports comparing Bl/6J Bl/6N (carrying wild-type Nnt allele) under normal diet have led conflicting results using tolerance tests. Thus, we assessed glucose-stimulated insulin secretion (GSIS),...
Hormones such as leptin and ghrelin can rapidly rewire hypothalamic feeding circuits when injected into rodent brains. These experimental manipulations suggest that the hypothalamus might reorganize continually in adulthood to integrate metabolic status of whole body. In this study, we examined whether plasticity occurs naive animals according their nutritional conditions. For purpose, fed mice with a short-term high-fat diet (HFD) assessed brain remodeling through its molecular functional...
Clinical and preclinical studies have implicated glial anomalies in major depression. Conversely, evidence suggests that the activity of antidepressant drugs is based, at least part, on their ability to stimulate density and/or astrocytes, a cell population. Despite this recent evidence, little known about mechanism(s) by which astrocytes regulate emotionality. Glial cells communicate with each other through gap junction channels (GJCs), while they can also directly interact neurons...
Glial cells have emerged as key players in the central control of energy balance and etiology obesity. Astrocytes play a role neural communication via release gliotransmitters. Acyl-CoA–binding protein–derived (ACBP-derived) endozepines are secreted peptides that modulate GABAA receptor. In hypothalamus, ACBP is enriched arcuate nucleus (ARC) astrocytes, ependymocytes, tanycytes. Central administration endozepine octadecaneuropeptide (ODN) reduces feeding improves glucose tolerance, yet...
Glucose is known to modify electrical activity of neurons in different hypothalamic areas such as the arcuate nucleus (ARC) or ventromedian nucleus. In these structures, it has been demonstrated that glucose-induced excitation involves ATP-sensitive K+ (KATP) channel closure. The aim present study was determine whether ARC were able detect high extracellular glucose concentrations and which mechanisms involved this detection by using whole-cell cell-attached patch-clamp techniques acute...
Fasting increases neuropeptide Y (NPY) expression, peptide levels, and the excitability of NPY-expressing neurons in hypothalamic arcuate (ARC) nucleus. A subpopulation ARC-NPY (∼40%) are glucose-inhibited (GI)-type glucose-sensing neurons. Hence, they depolarize response to decreased glucose. Because fasting enhances NPY neurotransmission, we propose that during fasting, GI smaller decreases This increased excitation glucose would increase NPY-GI neuronal enhance neurotransmission. Using an...
Hypothalamic pro-opiomelanocortin (POMC) neurons are known to trigger satiety. However, these neuronal cells encompass heterogeneous subpopulations that release γ-aminobutyric acid (GABA), glutamate, or both neurotransmitters, whose functions poorly defined. Using conditional mutagenesis and chemogenetics, we show blockade of the energy sensor mechanistic target rapamycin complex 1 (mTORC1) in POMC causes hyperphagia by mimicking a cellular negative state. This is associated with decreased...
Abstract The hypothalamus contains a remarkable diversity of neurons that orchestrate behavioural and metabolic outputs in highly plastic manner. Neuronal is key to enabling hypothalamic functions and, according the neuroscience dogma, it predetermined during embryonic life. Here, by combining lineage tracing pro-opiomelanocortin (Pomc) with single-cell profiling approaches adult male mice, we uncovered subpopulations ‘Ghost’ endowed atypical molecular functional identity. Compared...