A5062191903- Cell Adhesion Molecules Research
- Immune cells in cancer
- Melanoma and MAPK Pathways
- Ubiquitin and proteasome pathways
- Advanced Proteomics Techniques and Applications
- Hepatocellular Carcinoma Treatment and Prognosis
- Endoplasmic Reticulum Stress and Disease
- Phagocytosis and Immune Regulation
- Advanced X-ray Imaging Techniques
- Renal Transplantation Outcomes and Treatments
- Extracellular vesicles in disease
- Caveolin-1 and cellular processes
- Erythrocyte Function and Pathophysiology
- Digital Holography and Microscopy
- Protein Degradation and Inhibitors
- Signaling Pathways in Disease
- Lysosomal Storage Disorders Research
- Wnt/β-catenin signaling in development and cancer
- Optical Coherence Tomography Applications
- RNA Research and Splicing
- Blood groups and transfusion
- FOXO transcription factor regulation
- Complement system in diseases
- 14-3-3 protein interactions
- Cholangiocarcinoma and Gallbladder Cancer Studies
Inserm
2016-2025
Centre Hospitalier Universitaire de Bordeaux
2023-2024
Bordeaux Population Health
2018-2023
Université de Bordeaux
2018-2023
Translational Research in Oncology
2019
Université Claude Bernard Lyon 1
2016
Centre National de la Recherche Scientifique
2016
Centre de Recherche en Acquisition et Traitement de l'Image pour la Santé
2015-2016
Despite increasing demand, imaging the internal structure of plant organs or tissues without use transgenic lines expressing fluorescent proteins remains a challenge. Techniques such as magnetic resonance imaging, optical projection tomography X-ray absorption have been used with various success, depending on size and physical properties biological material.X-ray in-line phase was applied for structures maize seeds at early stages development, when cells are metabolically fully active water...
Invadosomes are F-actin-based structures involved in extracellular matrix degradation, cell invasion, and metastasis formation. Analyzing their proteome is crucial to decipher molecular composition, understand mechanisms, find specific elements target them. However, the analysis of invadosomes challenging, because it difficult maintain integrity during isolation. In addition, classical purification methods often suffer from contaminations, which may impair data validation. To ensure...
Until recently, 10% of hepatocellular adenomas (HCAs) remained unclassified (UHCA). Among the UHCAs, sonic hedgehog HCA (shHCA) was defined by focal deletions that fuse promoter Inhibin beta E chain with GLI1. Prostaglandin D2 synthase proposed as immunomarker. In parallel, our previous work using proteomic analysis showed most UHCAs constitute a homogeneous subtype associated overexpression argininosuccinate (ASS1). To clarify use ASS1 in classification and avoid misinterpretations...
Entosis is a process that leads to the formation of cell-in-cell structures commonly found in cancers. Here, we identified entosis hepatocellular carcinoma and loss Rnd3 (also known as RhoE) an efficient inducer this mechanism. We characterized different stages molecular regulators induced after silencing. demonstrated depends on RhoA/ROCK pathway, but not E-cadherin. The proteomic profiling entotic cells allowed us identify LAMP1 protein upregulated by silencing implicated only degradation...
Abstract Purpose Hepatocellular carcinoma (HCC) is the most common form of liver cancer with a bad prognosis in case advanced HCC, only eligible for palliative systemic therapies. After decade exclusive sorafenib monotherapy, response rate <10%, advent immunotherapies represents revolution HCC. The combination atezolizumab/bevacizumab recommended as first-line treatment, around 30%. However, there are currently no predictive factors to these treatment options. Experimental Design We...
Invasion is a prerequisite for metastasis formation. During tumor development, the extracellular matrix (ECM) remodeled in part through overexpression of type I collagen, increasing microenvironment stiffness, and facilitating cancer dissemination. breast cell migration, we observed membrane debris left behind, attached to collagen fibrils, along migration path. We named these structures collagen-tracks. These collagen-tracks can be deposited 3D matrices vitro vivo their formation stimulated...
Antibody-mediated rejection (ABMR) is the leading cause of allograft failure in kidney transplantation. Its histological hallmark represented by lesions glomerulitis i.e., inflammatory cells within glomeruli. Current therapies for ABMR fail to prevent chronic damage transplant glomerulopathy, loss. We used laser microdissection glomeruli from formalin-fixed biopsies combined with mass spectrometry-based proteomics describe proteome modification 11 active and 10 cases compared 8 stable graft...
Abstract Antibody-mediated rejection (ABMR) is the leading cause of allograft failure in kidney transplantation. Defined by Banff classification, its gold standard diagnosis remains a challenge, with limited inter-observer reproducibility histological scores and efficient immunomarker availability. We performed an immunohistochemical analysis 3 interferon-related proteins, WARS1, TYMP GBP1 cohort biopsies including 17 ABMR cases 37 other common graft injuries. Slides were interpreted, for...
Alpha-1 antitrypsin (AAT), encoded by the SERPINA1 gene, is a protein mainly produced and secreted hepatocytes. Some specific mutations affecting may cause accumulation of misfolded AAT in endoplasmic reticulum hepatocytes leading to deficiency (AATD). Z-AAT most severe common deficient variant. This mutant not only retained but accumulates as an aggregate that triggers cascade intracellular signalling pathways inducing hepatocyte injury death. Nevertheless, among all homozygous ZZ patients...
Background and Aims Through an exploratory proteomic approach based on typical hepatocellular adenomas (HCAs), we previously identified a diagnostic biomarker for distinctive subtype of HCA with high risk bleeding, already validated multicenter cohort. We hypothesized that the whole protein expression deregulation profile could deliver much more informative data tumor characterization. Therefore, pursued our analysis characterization profiles, evaluating their correspondence established...
A single point mutation in the Z-variant of alpha 1-antitrypsin (Z-AAT) alone can lead to both a protein folding and trafficking defect, preventing its exit from endoplasmic reticulum (ER), formation aggregates that are retained as inclusions within ER hepatocytes. These defects result systemic AAT deficiency (AATD) causes lung disease, whereas ER-retained induce severe liver injury patients with ZZ-AATD. Unfortunately, therapeutic approaches still limited transplantation represents only...
The association of immune thrombocytopenia (ITP) with cancer has been reported, but the causality tumor cells in paraneoplastic ITP pathogenesis and maintenance never established. We analyzed unusual case refractory coincident urothelial kidney circulating high titer anti-GPIIBIIIA autoantibodies. Intriguingly, after nephrectomy, patient recovered fully her autoantibodies disappeared. Proteomic immunohistochemistry analyses revealed erratic GPIIB expression by cells, suggesting possible...
Hepatocellular adenomas (HCAs) are rare, benign, liver tumours classified at the clinicopathological, genetic, and proteomic levels. The β-catenin-activated (b-HCA) subtypes harbour several mutation types in β-catenin gene (
The Src oncogene controls cancer cell invasiveness by promoting invadosome formation and extracellular matrix degradation (ECM). Invadosomes are enriched in the eukaryotic translation initiation factor 3 (eIF3) complex associated with a local mRNA activity mandatory for their maintenance. Here, we show that regulates controlling expression of eIF3 subunits. Among them, eIF3h/e/d essential ECM degradation. We demonstrate canonical mTOR/eIF4E non-canonical eIF3d cap-dependent pathways. both...
Abstract The ability to progress and invade through the extracellular matrix is a characteristic shared by both normal cancer cells formation of structures called invadosomes. These invadosomes are plastic dynamic that can adopt different organizations depending on cell types environment such as rosettes, dots or linear In this study, we used specific invadosome marker Tks5, identify common features in these organizations. Tks5 immunoprecipitation coupled with mass spectrometry analysis...
Previous studies have shown that Reptin is overexpressed in hepatocellular carcinoma and it necessary for vitro proliferation cell survival. However, its pathophysiological role vivo remains unknown. We aimed to study the of hepatocyte after regeneration using a liver knock-out model (ReptinLKO ). Interestingly, strongly impaired ReptinLKO mice 36 h partial hepatectomy, associated with decrease cyclin-A expression mTORC1 MAPK signalling, leading an regeneration. Moreover, model, we observed...
Abstract Entosis is a process that leads to the formation of cell-in-cell structures commonly found in cancers. Here, we identified entosis hepatocellular carcinoma and loss Rnd3 as an efficient inducer this mechanism. We characterized different stages molecular regulators induced after silencing. demonstrated depends on RhoA/ROCK pathway, but not E-cadherin. The proteomic profiling entotic cells allowed us identify LAMP1 protein upregulated by silencing implicated only degradation final...
Abstract Introduction Amyloidosis typing is crucial to determine the best therapeutic strategy for patients. Since conventional histological techniques often fail, identification of amyloid precursors by mass spectrometry became new standard. However, without quantification, selecting precursor from proteins that may be ubiquitous under non-pathological conditions equivocal. Therefore, we quantified protein enrichment in deposits improve typing. Methods Protein was measured extracted ion...
Abstract Invasion and migration through the extracellular matrix are prerequisites for metastasis, leading cause of cancer-related deaths. During tumor development, is remodeled, including by overexpressing type I collagen that facilitate cancer dissemination. Most studies have focused on events at edge as cells invade. We describe a new event trailing edge, detach from matrix. show small vesicles containing receptor DDR1 left behind fibrils in path. named these structures attached to fibers...