A5048292052- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Renal Transplantation Outcomes and Treatments
- Immunodeficiency and Autoimmune Disorders
- Complement system in diseases
- Renal Diseases and Glomerulopathies
- Cytomegalovirus and herpesvirus research
- Organ Transplantation Techniques and Outcomes
- Transplantation: Methods and Outcomes
- Cell Adhesion Molecules Research
- Reproductive System and Pregnancy
- Blood groups and transfusion
- Advanced biosensing and bioanalysis techniques
- Pregnancy and Medication Impact
- Immune Response and Inflammation
- vaccines and immunoinformatics approaches
- Hematopoietic Stem Cell Transplantation
- Glycosylation and Glycoproteins Research
- Platelet Disorders and Treatments
- Polyomavirus and related diseases
- HIV Research and Treatment
- Liver Disease and Transplantation
- Viral-associated cancers and disorders
Centre Hospitalier Universitaire de Bordeaux
2016-2025
Bordeaux Population Health
2020-2025
Hôpital Pellegrin
2016-2025
Inserm
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Université de Bordeaux
2016-2025
Immunology from Concept and Experiments to Translation
2015-2025
Centre Hospitalier Universitaire de Lille
2023-2024
Duke University Hospital
2022-2023
Duke Medical Center
2021-2023
Antibody-mediated rejection (AMR) is a major cause of kidney graft loss, yet assessment individual risk at diagnosis impeded by the lack reliable prognosis assay. Here, we tested whether capacity anti-HLA antibodies to bind complement components allows accurate stratification time AMR diagnosis. Among 938 transplant recipients for whom biopsy was performed between 2004 and 2012 Lyon University Hospitals, 69 fulfilled criteria were enrolled. Sera banked screened presence donor-specific (DSAs)...
HLA genotyping by next‐generation sequencing is now widely performed. We aimed at evaluating the performance of One Lambda AllType kit using Thermo Fisher Scientific reagents on Ion S5 XL platform. Reads were analyzed TypeStream Visual software. performed 15 runs between April and September 2018 to type DNA HLA‐A/B/C/DRB1/3/4/5/DQA1/DQB1/DPA1/DPB1 loci from 340 samples positive controls. observed only seven (0.1%) critical mistakes among 6009 alleles typed, corresponding two allele dropouts,...
C1q-binding ability may indicate the clinical relevance of de novo donor-specific anti-HLA antibodies (DSA). This study investigated incidence and risk factors for appearance DSA their long-term impact. Using Luminex Single Antigen Flow Bead assays, 346 pretransplant nonsensitized kidney recipients were screened at 2 5 years after transplantation DSA, which was followed when positive by a C1q assay. At years, 12 (3.5%) eight (2.5%) patients, respectively, had DSA. De mean fluorescence...
Anti-human leukocyte antigen (HLA) antibody detection in solid-phase flow beads assays can be quenched by complement activation, but the precise mechanism of this interference is not fully elucidated yet.Using Luminex screening assay for anti-HLA antibodies, we analyzed binding high concentrations pan class I monoclonal W6/32 neat normal, ethylenediaminetetraacetic acid-treated normal and factors C1q, C4/C3, C2, C3, factor B or C5-depleted human sera, using anti-mouse immunoglobulin G as...
Background Single antigen flow beads assays may overestimate sensitization because of the detection supposedly irrelevant antibodies recognizing denatured class I human leukocyte antigens (HLAs). Methods Sera 323 HLA-sensitized kidney transplant candidates positive with a HLA single assay were retested after acid treatment beads. Denatured identified according to ratio between measured fluorescence intensity for treated and nontreated T-lymphocyte cytometry crossmatches performed...
Polymerase chain reaction sequence‐specific oligonucleotide is commonly used for HLA‐typing. We replaced our LabType SSO HD (HD) kits with XR (XR) (One Lambda, Inc., Canoga Park, California) HLA‐A, ‐B, and ‐DRB1 following acquisition of a LABScan3D analyzer. The have more bead regions than the kits, allowing an extended number probes exon coverage. They are claimed to improve typing resolution diminish allele ambiguities, including common well‐documented (CWD) null alleles be resolved....
It is widely accepted that HLA donor-specific antibodies (DSA) are associated with antibody-mediated rejection and graft loss. However, in many transplant programs, preformed anti-HLA-Cw anti-HLA-DP DSA not considered organ allocation policies because their clinical relevance still uncertain.We analyzed the impact of Cw/DP through a retrospective study, comparing 48 patients transplanted isolated (Cw/DP group) (i) 104 matched HLA-sensitized kidney recipients No at D0 (No (ii) 47 A, -B, -DR,...
There is an ever-increasing need of monoclonal antibodies (mAbs) for biomedical applications and fully human binders are particularly desirable due to their reduced immunogenicity in patients. We have applied a strategy the isolation antigen-specific B cells using tetramerized proteins single-cell sorting followed by reconstruction mAbs RT-PCR expression cloning.This strategy, peripheral blood cells, enabled production low affinity against major histocompatibility complex molecules loaded...
ABSTRACT HLA‐DQA1*01:67:02 differs from HLA‐DQA1*01:67:01 by one nucleotide substitution in codon 229 exon 4.
ABSTRACT HLA‐A*03:01:130 differs from HLA‐A*03:01:01:01 by one nucleotide substitution in codon 266 exon 4.
ABSTRACT The systematic use of Single Antigen Flow Beads assays and the implementation high‐resolution HLA typing for donors kidney transplant recipients allow a precise identification anti‐HLA donor‐specific antibodies. In France, availability detailed molecular biology deceased in national organ allocation software enables anticipation wet crossmatch results estimation immunological risk recipient/donor pair. This key process, named virtual crossmatching, involves thorough analysis...
Anti-HLA donor-specific antibodies are deleterious for organ transplant survival. Class I HLA identified by using the Luminex single antigen beads (LSAB) assay, which also detects anti-denatured (anti-dHLAs). Anti-dHLAs thought to be unable recognize native (nHLA) on cell surface and therefore clinically irrelevant. Acid denaturation of nHLA LSAB allows anti-dHLAs discriminated from anti-nHLAs. We previously defined a threshold ratio between mean fluorescence intensity against acid-treated...
Class I single-antigen flow beads (SAFB) carry native and denatured human leukocyte antigen (HLA) molecules. Using a cohort of 179 class HLA-sensitized kidney recipients, we described incidence clinical relevance preformed HLA donor-specific antibodies (DSA) using two different assays: an acid-treated SAFB assay (anti-dHLA DSA) the iBeads assays (SAFB+/iBeads- DSA). Eighty-five DSA were found in 67 patients (median mean fluorescence intensity [MFI] 1729 [range 520-13 882]). Anti-dHLA...
Pathogenicity of donor-specific antibodies (DSAs) can be assessed using the single-antigen flow beads (SAFB) assays through mean fluorescence intensity (MFI) with or without serum ethylenediaminetetraacetic acid (EDTA) treatment, measurement C1q C3d binding and/or their intragraft detection [graft-bound antibody (gDSA)]. We aimed to investigate which these markers best associates antibody-mediated rejection (ABMR) and kidney allograft loss at time a for-cause biopsy.This retrospective,...
HLA‐DRB3*02:194 differs from HLA‐DRB3*02:02:01:02 by one nucleotide substitution in codon 78 exon 2.
HLA‐B*14:121 differs from HLA‐B*14:01:01:01 by one nucleotide substitution in codon 319 exon 6.
HLA-C*01:263 differs from HLA-C*01:02:01:01 by one nucleotide substitution in codon 98 exon 3.
HLA‐C*05:286 differs from HLA‐C*05:01:01:02 by one nucleotide substitution in codon 283 exon 5.
HLA‐B*51:394Q differs from HLA‐B*51:01:01:05 by one nucleotide substitution in codon 339 exon 7.
HLA-DRB1*04:04:20 differs from HLA-DRB1*04:04:01:04 by one nucleotide substitution in codon 135 exon 3.