A5035181538- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Fecal contamination and water quality
- Viral gastroenteritis research and epidemiology
- Vibrio bacteria research studies
- Water Treatment and Disinfection
- SARS-CoV-2 detection and testing
- Membrane Separation Technologies
- Aquaculture disease management and microbiota
- Urban Stormwater Management Solutions
- Biosensors and Analytical Detection
- Virus-based gene therapy research
- Water-Energy-Food Nexus Studies
- Child Nutrition and Water Access
- Salmonella and Campylobacter epidemiology
- Wastewater Treatment and Reuse
- Pharmaceutical and Antibiotic Environmental Impacts
- Respiratory viral infections research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Antibiotic Resistance in Bacteria
- Water Systems and Optimization
- Marine and coastal ecosystems
- Microbial infections and disease research
- Plant Virus Research Studies
- Groundwater flow and contamination studies
University of California, Irvine
2016-2025
Irvine University
2016-2025
University of California System
2005-2025
Samueli Institute
2006-2024
Beijing Solar Energy Research Institute
2022
Shanghai Jiao Tong University
2022
Irvine Valley College
2018
California Institute of Technology
2005-2018
Defence Research and Development Establishment
2011
Tsinghua University
2010
The paucity of proper sanitation facilities has contributed to the spread waterborne diseases in many developing countries. primary goal this study was demonstrate feasibility using a wastewater electrolysis cell (WEC) for toilet disinfection. treated designed reuse flushing and agricultural irrigation. Laboratory-scale electrochemical (EC) disinfection experiments were performed investigate efficiency WEC with four seeded microorganisms (Escherichia coli, Enterococcus, recombinant...
Rapid and cost-effective detection of antibiotics in wastewater through treatment processes is an important first step developing effective strategies for their removal. Surface-enhanced Raman scattering (SERS) has the potential label-free, real-time sensing antibiotic contamination environment. This study reports testing two gold nanostructures as SERS substrates label-free quinoline, a small-molecular-weight that commonly found wastewater. The results showed self-assembled substrate was...
ABSTRACT A nested-PCR method was used to detect the occurrence of human adenovirus in coastal waters Southern California. Twenty- forty-liter water samples were collected from 12 beach locations Malibu border Mexico between February and March 1999. All sampling sites located at mouths major rivers creeks. Two ultrafiltration concentration methods, tangential flow filtration (TFF) vortex (VFF), compared using six environmental samples. Human adenoviruses detected 4 tested after nucleic acid...
ABSTRACT To determine the potential for bacteriophage-mediated gene transfer in marine environment, we established transduction systems by using phage host isolates. Plasmid pQSR50, which contains transposon Tn 5 and encodes kanamycin streptomycin resistance, was used plasmid assays. Both bacterial isolates concentrated natural communities were as recipients studies. Transductants detected a probe complementary to neomycin phosphotransferase ( npt II) . The frequencies ranged from 1.33 × 10...
ABSTRACT Pathogenic bacteria and enteric viruses can be introduced into the environment via human waste discharge. Methods for rapid detection quantification of fecal indicator in water are urgently needed to prevent exposure pathogens through drinking recreational waters. Here we describe development two real-time PCR methods detect quantify adenoviruses enterococci environmental For enterococci, a set primers probe targeting 23S rRNA gene were used. The standard curve generated using...
To understand the role of viruses in marine environment, it is important to know factors affecting their temporal distribution and abundance lysogens.We therefore performed a seasonal diel study on viral Tampa Bay, Florida, USA, detected lysogens bacteriocinogens amongst bacterial isolates from diverse environments.We investigated viruses, direct counts, chlorophyll (chl a), salinity temperature during 13 mo period Bay estuary.The results indicated that population had strong pattern with...
ABSTRACT A total of 26 strains Vibrio cholerae , including members the O1, O139, and non-O1, non-O139 serogroups from both clinical environmental sources, were examined for presence genes encoding cholera toxin ( ctx A), zonula occludens zot ), accessory enterotoxin ace hemolysin hly NAG-specific heat-stable st toxin-coregulated pilus tcp outer membrane protein omp U), genomic organization, regulatory I tox R in order to determine relationships between epidemic serotypes sources isolation....
Vortex flow filtration (VFF) was used to concentrate viruses and dissolved DNA from freshwater seawater samples taken in Florida, the Gulf of Mexico, Bahamas Bank. Recoveries T2 phage calf thymus added artificial concentrated by VFF were 72.8 80%, respectively. Virus concentrations determined transmission electron microscopy VFF-concentrated ranged 3.4 x 10(7)/ml for a eutrophic Tampa Bay sample 2.4 10(5) an oligotrophic oceanic surface southeastern Mexico. Viruslike particles also observed...
ABSTRACT Vibrio cholerae is autochthonous to natural waters and can pose a health risk when it consumed via untreated water or contaminated shellfish. The correlation between the occurrence of V. in Chesapeake Bay environmental factors was investigated over 3-year period. Water plankton samples were collected monthly from five shore sampling sites northern (January 1998 February 2000) research cruise stations on north-south transect (summers 1999 2000). Enrichment used detect culturable ,...
Field studies were conducted to assess the coastal water quality impact of stormwater runoff from Santa Ana River, which drains a large urban watershed located in southern California. Stormwater river leads very poor surf zone quality, with fecal indicator bacteria concentrations exceeding California ocean bathing standards by up 500%. However, cross-shore currents (e.g., rip cells) dilute contaminated cleaner offshore, such that contamination is generally confined < 5 km around outlet....
Catchment urbanization perturbs the water and sediment budgets of streams, degrades stream health function, causes a constellation flow, quality, ecological symptoms collectively known as urban syndrome. Low-impact development (LID) technologies address hydrologic syndrome by mimicking natural flow paths restoring balance. Over annual time scales, volumes stormwater that should be infiltrated harvested can estimated from catchment-scale water-balance given local climate conditions preurban...
As more countries engage in water reuse, either intended or de facto, there is an urgent need to comprehensively evaluate resulting environmental and public health concerns. While antibiotic-resistant bacteria (ARB) antibiotic resistance genes (ARGs) are increasingly coming under the spotlight, as emerging contaminants, existing reuse regulations guidelines do not adequately address these This perspectives paper seeks frame various challenges that be resolved identify meaningful realistic...
Recycle domestic greywater for on-site non-potable uses can lessen the demand on potable water and burden wastewater treatment plants. However, lack of studies to assess health risk associated with such practices has hindered their popularity. A Quantitative Microbial Risk Assessment was conducted estimate public risks two reuse scenarios: toilet flushing food-crop irrigation. Household quality from three sources (bathroom, laundry kitchen) analyzed. Mathematical exposure rates different...
Agricultural irrigation using reclaimed urban wastewater (RWW) represents a sustainable practice to meet the ever-increasing water stress in modern societies. However, occurrence of residual antibiotics and antibiotic resistant bacteria (ARB) RWW is an important human health concern. This study applied for first time novel Simple-Death dose-response model field data Escherichia coli Pseudomonas spp. collected from three greenhouses cultivation tomatoes irrigated with RWW. The estimates risk...
MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 142:27-38 (1996) - doi:10.3354/meps142027 Occurrence of lysogenic bacteria in marine microbial communities as determined by prophage induction Jiang SC, Paul JH Viruses are abundant and dynamic members community, it is important understand their role ecology natural populations. We have previously found be a...
The distribution of viral and microbial abundance in the Key Largo, Fla., reef environment was measured. Viral measured by transmission electron microscope direct counts plaque titer on specific bacterial hosts water sediment samples from Florida Bay (Blackwater Sound) along a transect Largo to outer edge tract Sanctuary. Water column were highest Blackwater Sound (1.2 x 10(7) viruses per ml), decreased shelf break (1.7 10(6) inversely correlated with salinity (r = -0.97). ranged 1.35 10(8)...
ABSTRACT Real-time PCR was applied to quantify the abundance of human adenoviruses in two southern California urban rivers, San Gabriel and Los Angeles. A total 114 river samples from five different locations were collected over a 1-year period analyzed for adenoviruses, along with fecal indicator bacteria coliphages. Adenoviruses detected by real-time ∼16% samples, concentrations ranging 10 2 4 genomes per liter. However, plaque assay using tissue culture cell lines, HEK-293A A549, yielded...