A5100600992- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
- Advanced biosensing and bioanalysis techniques
- Angiogenesis and VEGF in Cancer
- Dendrimers and Hyperbranched Polymers
- Extracellular vesicles in disease
- Zebrafish Biomedical Research Applications
- Liver physiology and pathology
- CRISPR and Genetic Engineering
- Eicosanoids and Hypertension Pharmacology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Metabolomics and Mass Spectrometry Studies
- Nerve Injury and Rehabilitation
- Telomeres, Telomerase, and Senescence
- Spaceflight effects on biology
- Congenital heart defects research
- Vascular Malformations and Hemangiomas
- MicroRNA in disease regulation
- High Altitude and Hypoxia
- Immunodeficiency and Autoimmune Disorders
- Acute Myeloid Leukemia Research
- Nanoparticle-Based Drug Delivery
- Lipid metabolism and disorders
- Muscle Physiology and Disorders
- Analytical Chemistry and Chromatography
Southeast University
2024-2025
Zhejiang University
2023
Shanghai Jiao Tong University
2021
Shanghai Ninth People's Hospital
2021
BGI Group (China)
2020
Binzhou University
2020
Binzhou Medical University
2020
China National GeneBank
2020
University of California, San Diego
2018
Dalian Medical University
2016
Lipid nanoparticles (LNP) have shown great promise in clinical applications for delivering mRNA. Targeted delivery of mRNA to particular tissues or organs is essential precise therapeutic outcomes and minimized side effects various disease models. However, achieving targeted beyond the liver a challenge based on current LNP formulations. In this report, we synthesized four ionizable cholesterol analogs by attaching two tertiary amine groups onto head cholesterol-like structure incorporated...
Abstract Lipoprotein lipase (LPL) mediates hydrolysis of triglycerides (TGs) to supply free fatty acids (FFAs) tissues. Here, we show that LPL activity is also required for hematopoietic stem progenitor cell (HSPC) maintenance. Knockout Lpl or its obligatory cofactor Apoc2 results in significantly reduced HSPC expansion during definitive hematopoiesis zebrafish. A human APOC2 mimetic peptide the very low-density lipoprotein, which carries APOC2, rescues phenotype apoc2 but not lpl mutant...
PEAK1 is a newly described tyrosine kinase and scaffold protein that transmits integrin-mediated extracellular matrix (ECM) signals to facilitate cell movement growth. While aberrant expression of has been linked cancer progression, its normal physiological role in vertebrate biology not known. Here we provide evidence plays central orchestrating new vessel formation vertebrates. Deletion the gene zebrafish, mice, human endothelial cells (ECs) induced severe defects blood due deficiencies EC...
Abstract Background To investigate the therapeutic effect of human dental pulp stem cells (DPSCs) transfected with adenovirus expressing hepatocyte growth factor (HGF) in a mouse model collagen-induced arthritis (CIA). Methods DPSCs were modified Ad-HGF to produce HGF-overexpressing DPSCs, DPSCs-HGF. In experimental CIA model, DPSCs-HGF and DPSCs-Null (modified Ad-Null) engrafted via intravenously after disease onset, which was determined by presence joint swelling. The effects on joints...
Though infantile hemangioma (IH) is a common benign vascular tumor, its pathogenesis remains unclear. This study explored the function of hemangioma-derived stem cells (HemSCs) derived exosomes, which exerted an intercellular effect on endothelial (HemECs).First, HemSCs and HemECs were extracted cultured. exosomes (HemSCs-exos) harvested. miRNA sequencing target prediction used to explore differentially expressed miRNAs potential binding targets. After co-cultured with HemSCs-exos, series in...
Abstract Conditions in space, such as microgravity, may affect the hematopoietic and bone marrow‐derived mesenchymal stromal cells (BM‐MSCs) of astronauts. However, to date, few detailed phenotype change data about different type have reported. In this study, C57BL/6 mice were randomly divided into two groups: a control group (control) hindlimb suspension (treated). After four weeks suspension, we found that simulated microgravity (sµg) condition could increase percentage monocytes...
Abstract Radiation therapy can cause haematopoietic damage, and mesenchymal stem cells (MSCs) derived extracellular vesicles (EVs) have been shown to reverse this damage. Our previous research showed that dental pulp (DPSCs) a strong proliferation capacity produce abundant amounts of EVs meet the requirements for use in vitro vivo. DPSCs (DPSCs-EVs) are evaluated their effect on reducing Haematopoietic cell (HSC) numbers function were assessed by flow cytometry, peripheral blood counts,...
Spinal cord injury (SCI) is one of the most complex and destructive diseases nervous system, which can lead to permanent loss tactile perception. But existing treatment methods have limited effects. To establish a novel method that may be therapeutic in repairing injured spinal cord, gene-modified dental pulp stem cells (DPSCs) were injected situ. Adenovirus carrying osteopontin (OPN), Insulin-like growth factor 1 (IGF-1) cailiary-derived neurotrophic (CNTF) (Ad-OIC) was constructed. After...
Retinoic acid (RA) is a widely-used agent inducing cell differentiation. In order to study the effect of excessive RA on myogenesis, C2C12 cells were cultured in gradient concentrations RA. this study, cycle arrested G0/G1 phase when concentration was higher than 10 µM. Cell proliferation inhibited by over 40 Therefore, 1 and µM chosen treat under serum withdrawal, respectively, then, myogenic regulatory factors (MRFs) measured real-time PCR. It found that after treatment, Myf5 transcription...
Abstract β-thalassemia is one of the most prevalent genetic diseases worldwide. The current treatment for β–thalassemia allogeneic hematopoietic stem cell transplantation (HSCT), which limited due to lack matched donors. Gene therapy has been developed as an alternative therapeutic option transfusion-ependent β -thalassemia (TDT). However, successful gene patients in China not reported. Here we present results preclinical studies optimally designed LV named LentiHBB T87Q cells (HSCs) derived...