Abstract Estrogens play a crucial role in the development of ovarian tumors; however, signal transduction pathways involved hormone action are still poorly defined. The orphan G protein–coupled receptor 30 (GPR30) mediates nongenomic signaling 17β-estradiol (E2) variety estrogen-sensitive cancer cells through activation epidermal growth factor (EGFR) pathway. Whether estrogen α (ERα) also contributes to GPR30/EGFR is less understood. Here, we show that, ERα-positive BG-1 cells, both E2 and...

The higher incidence of thyroid carcinoma (TC) in women during reproductive years compared with men and the increased risk associated therapeutic use estrogens have suggested a pathogenetic role exerted by these steroids development TC. In present study, we evaluated potential 17β-estradiol (E2), genistein (G), 4-hydroxyta-moxifen (OHT) to regulate expression diverse estrogen target genes proliferation human WRO, FRO, ARO cells, which were used as model system. We ascertained that cells are...

Human and animal models have evidenced how estrogen insufficiency is associated with abnormal spermatogenesis male infertility. We previously demonstrated that estradiol able to influence both capacitation acrosome reaction in human ejaculated spermatozoa. It remains be elucidated whether the biochemical changes induced by estradiol, a rapid nongenomic way, are mediated single receptor (ER) or two ER subtypes, ERα ERβ. In present study, we first concomitant expression of ERβ By RT-PCR...

Antioxidant capacity of seminal plasma was evaluated in 120 semen samples subdivided into asthenozoospermic and oligoasthenozoospermic specimens with normal consistency hyperviscosity. Semen (n = 25) from normozoospermic donors were used as a control group. Scavenger antioxidant reactive oxygen species by superoxide dismutase catalase activity measurements, whereas the chain-breaking efficiency detected total status assessment. In viscosity, unaltered enzymatic nonenzymatic revealed...

Abstract Background The presence of small membranous particles characterizes the male genital fluids different mammalian species. influence semen vesicles, denominated prostasomes, on sperm functional properties has been well documented in humans, but their biological activity is scarcely known other present work investigated prostasome-like vesicles pig for ability to interact with spermatozoa and affect acrosome reaction. Methods Prostasome-like have isolated from seminal plasma by...

Abstract Background Estrogen signaling is mediated by estrogen receptor beta isoforms in normal and neoplastic human testes. Recently, a G-protein-coupled-receptor (GPER) has been suggested as being involved rapid responses to estrogens different tumor cells. Methods This study investigated the GPER expression paraffin-embedded samples from non testes (sex-cord stromal germ cell tumors) immunohistochemical Western Blot analyses. Results In control testes, positive immunoreactivity was...

Previous epidemiological reports have suggested that red wine intake is associated with beneficial health effects due to the ability of certain phytochemical components exert estrogen-like activity. It has been also documented estrogens induce proliferation hormone-dependent breast cancer cells by binding and transactivating estrogen receptor (ER) α, which in turn interacts responsive DNA sequences located within promoter region target genes. In order provide further insight into positive...

Several studies have recently investigated the role of leptin, adipocyte-secreted hormone, in growth and reproduction rodents, humans, domestic animals. The present study was designed to explore expression leptin its receptor pig spermatozoa. Successful Western blot evidenced a 16 kDa band for six isoforms, ranging from 120 40 kDa, receptor. Both were interestingly located at sperm acrosomal level, suggesting their involvement oocyte fertilization events. In fact, both capacitation indexes...

Abstract Cytochrome P450 aromatase is a terminal enzyme that catalyses the conversion of androgens into oestrogens. This study investigated immunohistochemical localization in human efferent ductules and proximal ductus epididymis using mouse anti‐human monoclonal P450arom IgG as primary antibody goat anti‐mouse biotinylated secondary antibody. A strong immunoreaction was observed epithelial cell cytoplasm both ductuli efferentes epididymis, whereas smooth muscle cells were immunonegative...

Androgens and estrogens are crucial for mammalian sperm differentiation but their role in biology of mature male gamete is not still defined. The expression proteins involved the biosynthesis action these steroid hormones has been demonstrated human spermatozoa, very few data have reported from non species. purpose current study was to investigate aromatase (P450arom), estrogen (ERalpha/ERbeta) androgen (AR) receptors ejaculated spermatozoa pig.The immunfluorescence experiments were carried...

A Leydig cell tumor is a rare neoplasm, deriving from the interstitial cells, whose pathogenesis has not been still defined. cells of normal adult testis are known as physiological targets for estrogens. However, some studies on transgenic rodents suggest role estrogens in development hyperplasia and tumor. Therefore, with aim to evaluate possible link between testicular tumorigenesis, this study investigated expression aromatase estrogen receptors (ERα, ERβ 1 , 2 ) testes two patients...

Abstract Background Nitric oxide (NO) is a signaling molecule produced by intracellular nitric synthase (NOS) enzymes. This free radical appears to affect sperm capacitation, maturation step preceding acrosome reaction. Recent studies have reported leptin ability promote capacitation and reaction in pig male gametes. Methods study has investigated production leptin-treated spermatozoa fluorescence-activated cell sorting, while the NOS isoforms were assessed Western blot analysis. In...

Abstract Estrogens are known to influence functional properties of mammalian spermatozoa inducing rapid responses through the classical estrogen receptors ( ER α and β). Recently, G protein‐coupled receptor GPER) has been identified as mediator fast non‐genomic effects in different cells. This work investigated expression GPER human pig using immunofluorescence, W estern b lot analysis RT ‐ PCR . was found be confined mid‐piece sperm cells, whereas it detected acrosomal region, equatorial...

Estrogens and thyroid hormones play a significant role in regulating functions development of the testis. The synthesis estrogens from androgens is catalyzed by enzyme complex termed aromatase, which testis displays an age-related cellular compartmentalization, primarily Sertoli cells immature animals, whereas adults it expressed Leydig germ cells. T3 induces precocious terminal differentiation prepubertal together with dramatic decrease their aromatase activity. In present work, we have...

Total seminal zinc concentration, fraction bound to high molecular weight proteins (HMW-Zn%) and content in spermatozoa were assayed the ejaculates of 90 asthenozoospermic patients subdivided into two study groups: normoasthenozoospermics (group I: n = 50) oligoasthenozoospermics II: 40). The concentrations compared with those a control group donors showing normal semen parameters. All samples also investigated for their sperm membrane functional integrity by hypo-osmotic swelling test...

Our recent studies have revealed that estrogens stimulate an autocrine mechanism determining Leydig tumor cell proliferation. Estrogen overproduction is due to elevated steroidogenic factor-1 (SF-1) expression and cAMP-response element-binding protein (CREB) phosphorylation, both inducing aromatase overexpression. Although we shown increased SF-1 depends mainly on higher local insulin-like growth factor I production, the mechanisms factors CREB activation in cells are not completely...

Recent studies have revealed that insulin, the main regulator of glucose homeostasis in somatic cells, is expressed human spermatozoa which are also able to secrete it. This study investigated expression insulin and receptor beta pig spermatozoa, at immunohistochemical protein mRNA level. The immunofluorescence assay its were co-localized sperm midpiece, while was detected acrosomal region. Western blot evidenced a 36 kDa band for 95 receptor, such as reported cells. In addition, both...

B induced a lowering of PI3K/AKT survival signal in MCF‐7 cells even presence IGF‐I stimulation. Furthermore, and higher extent, PUVA up‐regulated the p53 mRNA protein content. An increased co‐association between p21 WAF proliferating cell nuclear antigen (PCNA) has been observed PUVA‐treated cells, thus inhibiting DNA replication. These results highlight how B, its photoactivated compound, exert antiproliferative effects induce apoptotic responses breast cancer cells.

Summary Estrogens are involved in growth, differentiation and pathogenesis of human prostate through the mediation classical estrogen receptors ER α β. The G protein‐coupled receptor ( GPER ) is a ‘novel’ mediator signaling which has been recently recognized some reproductive tissues, but its expression gland still unknown. Here, we investigated benign (from 5 patients) neoplastic prostatic tissues 50 by immunohistochemical analysis Western blotting. Normal areas prostates revealed strong...

High consumption of soybean products, such as phytoestrogens, has been hypothesized to contribute a reduced incidence prostate cancer in Southeast Asian people, although there have inconsistent results among studies. Human LNCaP cells, extensively used model for androgen-dependent tumor, express the androgen receptor (AR) mutant T877A promiscuously transactivated by estrogens and other ligands, which may further facilitate progression. Here, first time our knowledge, we demonstrate that...

The role of oestrogens in the development prostate cancer is poorly understood. However, a large body evidence has suggested that oestrogenic hormones may be involved prostatic malignancy. localization oestrogen receptor beta (ERbeta) secretory epithelium human raised intriguing possibility action could mediated, at least part, by this during process carcinogenesis. Hence, specific interference with oestrogen-activated and ERbeta-mediated transcriptional activity open new issues endocrine...