A5052627515- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
- Skin Protection and Aging
- Spectroscopy Techniques in Biomedical and Chemical Research
- Laser Applications in Dentistry and Medicine
- Optical Imaging and Spectroscopy Techniques
- Optical Coherence Tomography Applications
- Porphyrin and Phthalocyanine Chemistry
- Bacterial biofilms and quorum sensing
- Oral and Maxillofacial Pathology
- Oral Health Pathology and Treatment
- Ultrasound and Cavitation Phenomena
- Oral microbiology and periodontitis research
- bioluminescence and chemiluminescence research
- Advanced Fiber Laser Technologies
- Digital Holography and Microscopy
- Cell Image Analysis Techniques
- Bee Products Chemical Analysis
- Spectroscopy and Chemometric Analyses
- Insect Pest Control Strategies
- Diamond and Carbon-based Materials Research
- Dental Research and COVID-19
- Cutaneous Melanoma Detection and Management
Universidade de São Paulo
2015-2024
Universidade Federal de São Carlos
2013-2024
Universidade Federal do Espírito Santo
2024
Institute of Physics
2024
Universidad Nacional Autónoma de México
2020
Universidade Federal do Pampa
2020
Cepsa (Spain)
2020
Universidade de Brasília
2020
City College of New York
2013-2014
Hospital de Clínicas da Unicamp
2013
Light at wavelengths in the near-infrared (NIR) region allows for deep penetration and minimal absorption through high scattering tissue media. NIR light has been conventionally used first optical window with from 650 to 950 nm. Longer had overlooked due major water peaks a lack of NIR-CCD detectors. The second spectral 1100 1350 nm new 1600 1870 nm, known as third window, were investigated. Optical attenuation measurements thin slices normal malignant breast prostate tissues, pig brain,...
Zinc (Zn)-based nanoparticles (NPs) can be potentially used in medical and food preservation applications. As proof of principle, we investigated the bactericidal antibiofilm activity zinc oxide (ZnO) sulfide (ZnS) NPs against medically important bacteria.
Spatially coherent multicolored optical vector vortex beams were created using a tunable liquid crystal q-plate and supercontinuum light source. The feasibility of the as spectral filter (switch) was demonstrated, polarization topology resulting beam mapped. Potential applications include multiplexing for broadband high-speed communication, ultradense data networking, super-resolution microscopy.
This work evaluated antimicrobial photodynamic therapy (PDT), sonodynamic (SDT), and the association of both therapies (sonophotodynamic [SPDT]), mediated by curcumin (Cur) against Staphylococcus aureus biofilm. Next, additional strategies for these treatments were assessed.S. biofilms received PDT, SDT, SPDT, Cur (80 µM), LED light (450 nm), 1 MHz ultrasound. The same also performed adding a strategy: with sodium dodecyl sulfate (SDS), potassium iodide (KI) or pre-treatment Cell viability...
Photodynamic therapy (PDT) relies on a series of photophysical and photochemical reactions leading to cell death. While effective for various cancers, PDT has been less successful in treating pigmented melanoma due high light absorption by melanin. Here, this limitation is addressed 2-photon excitation the photosensitizer (2p-PDT) using ~100 fs pulses near-infrared laser light. A critical role melanin enabling rather than hindering 2p-PDT elucidated non-pigmented murine clonal lines vitro....
Melanoma is the most aggressive skin cancer type.It characterized by pigmented lesions with high tissue invasion and metastatic potential.The early detection of melanoma extremely important to improve patient prognosis survival rate, since it can progress deadly stage.Presently, diagnosis based on clinical analysis macroscopic lesion characteristics such as shape, color, borders following ABCD rules.The aim this study evaluate time-resolved fluorescence lifetime NADH FAD molecules detect...
This study evaluated the antibacterial activity of terpinen-4-ol against Streptococcus mutans and Lactobacillus acidophilus its influence on gbpA (S. mutans) slpA (L. acidophilus) gene expression. As measured by XTT assay, concentrations that effectively inhibited biofilm were 0.24% 0.95% for S. L. acidophilus, respectively. Confocal microscopy revealed presence a attached to enamel dentin block surfaces with significant effects these microorganisms. The expression genes involved in...
Two-photon (2P) excitation of the second singlet (S2) state a group fluorescent agents with near infrared emission was used to extend optical and imaging regime 2P microscope into “tissue window” (650–1100 nm). As first step achieve deeper imaging, Chlorophyll Indocyanine green are investigated demonstrated as using S2 at 800 nm for 685 nm. The salient feature is drive both wavelengths fall window.”
Objective: In this study, we aim to compare the photodynamic inactivation (PDI) effects of two different photosensitizers (PS), Photogem® and Natural Curcumin, irradiated with light-emitted diodes (LED) at 630 450 nm, respectively. Background: The current antimicrobial mouthwash for oral hygiene has several drawbacks. context, PDI is an alternative technique inactivate pathogenic microbes in mucosa periodontal tissue. Furthermore, there are numerous infectious diseases that may affect...
Actinic cheilitis is a potentially malignant disorder of the lips.Its first cause believed to be UV sun radiation.The lesion highly heterogeneous, making choice area biopsied difficult.This study exploits capabilities time-resolved fluorescence spectroscopy for identification most representative biopsied.A preliminary was performed on fourteen patients.A classification algorithm used data acquired nine different biopsies.The discriminated between absent, mild, and moderate dysplasia with...
With the aim of contributing to development more efficient materials for wound care, new topical formulations based on bacterial nanocellulose (BNC) hydrogels containing propolis were produced. Characterizations confirmed incorporation into BNC matrix, maintaining its structure and properties. Rheological analysis that showed thixotropic behavior appropriate application. Chromatographic profiles sustained release biomarkers at least 20 h. The did not present mutagenicity. For application in...
Tryptophan is investigated as the key native marker in cells to determine level of metastasis competence breast cell lines using fluorescence spectroscopy. The ratio intensity at 340 nm 460 associated with aggressiveness cancer cells. We found that aggressive has a much higher contribution from tryptophan compared normal and nonaggressive cell.
Strain plays an important role for the optical properties of monolayer transition metal dichalcogenides (TMDCs). Here, we investigate strain effects in a MoSe2 sample with large bubble region using μ-Raman, second harmonic generation (SHG), μ-photoluminescence and magneto at low temperature. Remarkably, our results reveal presence non-uniform field observation emission peaks lower energies which are signatures exciton trion quasiparticles red-shifted by region, agreement theoretical...
Light at wavelengths in the visible and near-infrared (NIR) regions (650 nm – 1,350 nm) is used numerous medical applications. The NIR region between 650 950 nm, known as first optical window, allows for deeper depth penetration tissue than due to a reduction absorption. There also exists second window with from 1,100 nm. Longer above were ignored major water absorption lack of 2D photodetectors. In this study, new therapeutic spectral 1,600 1,870 reported. This third can be imaging more...
Photodynamic therapy (PDT) is a treatment modality that can be indicated for several cancer types and pre-cancer lesions. One of the main applications PDT superficial skin lesions such as basal cell carcinoma, Bowen's disease actinic keratosis. Three elements are necessary in PDT, photosensitizer (PS); light at specific wavelength to absorbed by PS, molecular oxygen. A typical PS used lesion protoporphyrin IX (PpIX), which an intrinsic PS; its production stimulated pro-drug, 5-aminolevulinic...
Fluorescence spectroscopy and lifetime techniques are potential methods for optical diagnosis characterization of biological tissues with an in-situ, fast, noninvasive interrogation. Several diseases may be diagnosed due to differences in the fluorescence spectra targeted fluorophores, when, these similar, considering steady-state fluorescence, others detected by monitoring their lifetime. Despite this complementarity, most current systems not robust portable, being feasible clinical...
The inactivation of pathogenic microorganisms that are a threat to the human being is an old challenge needs be overcome, mainly because able develop resistance leads failure treatment. Moreover, biofilm living form complex microorganism community less susceptible antimicrobials agents. For this reason, search for alternative therapies not induce and have ability inactivate biofilms unquestionably needed. In review, Photodynamic Sonodynamic Inactivation (PDI SDI) suggested as promising...
Photobiomodulation therapy (PBMT) uses light to stimulate cells. The molecular basis of the effects PBMT is being unveiled, but it stated that cytochrome-c oxidase enzyme in mitochondria, a photon acceptor PBMT, contributes an increase ATP production and modulates reduction oxidation electron carriers NADH FAD. Since its are not fully understood, used on tumors. Thus, interesting investigate if correlate mitochondrial metabolism so, how could be linked optical redox ratio (ORR), defined as...