A5085648748- RNA Research and Splicing
- RNA modifications and cancer
- Cancer-related gene regulation
- Invertebrate Immune Response Mechanisms
- Developmental Biology and Gene Regulation
- Neurobiology and Insect Physiology Research
- Microtubule and mitosis dynamics
- CRISPR and Genetic Engineering
- Physiological and biochemical adaptations
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Genetics, Aging, and Longevity in Model Organisms
- DNA Repair Mechanisms
- Mitochondrial Function and Pathology
- RNA regulation and disease
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
Stanford University
2021-2025
University of Manchester
2020-2022
Manchester Academic Health Science Centre
2020
Regulators of chromatin accessibility play key roles in cell fate transitions, triggering onset novel transcription programs as cells differentiate. In the Drosophila male germ line stem lineage, tMAC, a master regulator spermatocyte differentiation that binds thousands loci, is required for local opening chromatin, allowing activation spermatocyte-specific promoters. Here we show cell-type specific surveillance system involving multiple zinc finger protein Kmg and pipsqueak domain Dany...
Alternative polyadenylation (APA) generates transcript isoforms that differ in the position of 3′ cleavage site, resulting production mRNA with different length UTRs. Although widespread, role APA biology cells, tissues, and organisms has been controversial. We identified >500 Drosophila genes express a long UTR proliferating spermatogonia but short differentiating spermatocytes due to APA. show stage-specific choice end site can be regulated by arrangement canonical signal (PAS) near...
Alternative cleavage and polyadenylation (APA) often results in production of mRNA isoforms with either longer or shorter 3′ UTRs from the same genetic locus, potentially impacting translation, localization, stability. Developmentally regulated APA can thus make major contributions to cell type-specific gene expression programs as cells differentiate. During Drosophila spermatogenesis, ∼500 genes undergo when proliferating spermatogonia differentiate into spermatocytes, producing transcripts...
Abstract The Drosophila melanogaster peripheral nervous system (PNS) comprises the sensory organs that allow fly to detect environmental factors such as temperature and pressure. PNS development is a highly specified process where each sensilla originates from single organ precursor (SOP) cell. One of major genetic orchestrators Senseless, which encodes zinc finger transcription factor (Sens). Sens both necessary sufficient for SOP differentiation. Senseless expression number are regulated...
During meiosis, germ cell and stage-specific components impose additional layers of regulation on the core cycle machinery to set up an extended G2 period termed meiotic prophase. In Drosophila males, prophase lasts 3.5 days, during which spermatocytes upregulate over 1800 genes grow 25-fold. Previous work has shown that regulator Cyclin B (CycB) is subject translational repression in immature spermatocytes, mediated by RNA-binding protein Rbp4 its partner Fest. Here, we show...
Alternative Cleavage and Polyadenylation (APA) often results in production of mRNA isoforms with either longer or shorter 3'UTRs from the same genetic locus, potentially impacting translation, localization stability. Developmentally regulated APA can thus make major contributions to cell-type-specific gene expression programs as cells differentiate. During
The switch from precursor cell proliferation to onset of differentiation in adult stem lineages must be carefully regulated produce sufficient progeny maintain and repair tissues, yet prevent overproliferation that may enable oncogenesis. In the
Abstract MicroRNAs have subtle and combinatorial effects on the expression levels of their targets. Studying consequences a single microRNA knockout often proves difficult as many such knockouts exhibit phenotypes only under stress conditions. This has led to hypothesis that microRNAs frequently act buffers noise in gene expression. Observing understanding buffering requires quantitative analysis target cells. To this end, we employed molecule fluorescence situ hybridization,...
ABSTRACT During meiosis, germ cell and stage-specific components impose additional layers of regulation on the core cycle machinery to set up an extended G2 period termed meiotic prophase. In Drosophila males, prophase lasts 3.5 days, during which spermatocytes turn expression over 3000 genes grow 25-fold in volume. Previous work showed that regulator Cyclin B (CycB) is subject translational repression immature spermatocytes, mediated by RNA-binding protein Rbp4 its partner Fest. Here we...
Abstract The Drosophila melanogaster peripheral nervous system (PNS) comprises the sensory organs that allow fly to detect environmental factors such as temperature and pressure. PNS development is a highly specified process where each sensilla originates from single organ precursor (SOP) cell. One of major genetic orchestrators Senseless , which encodes zinc finger transcription factor (Sens). Sens both necessary sufficient for SOP differentiation. expression number are regulated by...
MicroRNAs can have subtle and combinatorial effects on the levels of targets pathways they act on. Studying consequences a single microRNA knockout often proves difficult as many such knockouts exhibit phenotypes only under stress conditions. This has led to hypothesis that microRNAs buffer intrinsic environmental stochasticity gene expression. Observing understanding this buffering effect entails quantitative analysis target expression in cells. To end, we employed single-molecule...
Abstract Alternative polyadenylation (APA) generates transcript isoforms that differ in the position of 3’ cleavage site, resulting production mRNA with different length 3’UTRs. Although widespread, role APA biology cells, tissues and organisms has been controversial. We identified over 500 Drosophila genes express a long 3’UTR proliferating spermatogonia but short differentiating spermatocytes due to APA. show stage-specific choice end site can be regulated by arrangement canonical signal...