A5047362244- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- RNA Research and Splicing
- SARS-CoV-2 detection and testing
- Pluripotent Stem Cells Research
- Mosquito-borne diseases and control
- Click Chemistry and Applications
- Biosensors and Analytical Detection
- Circular RNAs in diseases
- RNA regulation and disease
- RNA modifications and cancer
Institute of Genomics and Integrative Biology
2018-2024
Academy of Scientific and Innovative Research
2019-2023
Council of Scientific and Industrial Research
2019-2020
Rapid detection of DNA/RNA pathogenic sequences or variants through point-of-care diagnostics is valuable for accelerated clinical prognosis, as witnessed during the recent COVID-19 outbreak. Traditional methods relying on qPCR sequencing are tough to implement with limited resources, necessitating development accurate and robust alternative strategies. Here, we report FnCas9 Editor Linked Uniform Detection Assay (FELUDA) that utilizes a direct Cas9 based enzymatic readout detecting...
Genome editing using the CRISPR/Cas9 system has been used to make precise heritable changes in DNA of organisms. Although widely Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants have efficiently harnessed for numerous gene-editing applications across different platforms, concerns remain regarding their putative off-targeting at multiple loci genome. Here we report that Francisella novicida (FnCas9) shows a very high specificity binding intended targets negligible off-target...
Abstract The clinical success of CRISPR therapies hinges on the safety and efficacy Cas proteins. Cas9 from Francisella novicida (FnCas9) is highly precise, with a negligible affinity for mismatched substrates, but its low cellular targeting efficiency limits therapeutic use. Here, we rationally engineer protein to develop enhanced FnCas9 (enFnCas9) variants broaden their accessibility across human genomic sites by ~3.5-fold. enFnCas9 proteins single mismatch specificity expanded target...
Detection of pathogenic sequences or variants in DNA and RNA through a point-of-care diagnostic approach is valuable for rapid clinical prognosis. In recent times, CRISPR based detection nucleic acids has provided an economical quicker alternative to sequencing-based platforms which are often difficult implement the field. Here, we present FnCas9 Editor Linked Uniform Assay (FELUDA) that employs highly accurate enzymatic readout detecting nucleotide sequences, identifying nucleobase identity...
Clustered miRNAs can affect functioning of downstream pathways due to possible coordinated function. We observed 78-88% the miR-379/miR-656 cluster (C14MC) were downregulated in three sub-types diffuse gliomas, which was also corroborated with analysis from The Cancer Genome Atlas (TCGA) datasets. miRNA expression levels decreased increasing tumor grade, indicating this downregulation as an early event gliomagenesis. Higher C14MC significantly improved glioblastioma prognosis (Pearson's r =...
ABSTRACT Rapid detection of pathogenic sequences or variants in DNA and RNA through a point-of-care diagnostic approach is valuable for accelerated clinical prognosis as has been witnessed during the recent COVID-19 outbreak. Traditional methods relying on qPCR sequencing are difficult to implement settings with limited resources necessitating development accurate alternative testing strategies that perform robustly. Here, we present FnCas9 Editor Linked Uniform Detection Assay (FELUDA)...
Embryonic stem cells (ESCs) can undergo lineage-specific differentiation, giving rise to different cell types that constitute an organism. Although roles of transcription factors and chromatin modifiers in these have been described, how the alternative splicing (AS) machinery regulates their expression has not sufficiently explored. Here, we show long non-coding RNA (lncRNA)-associated protein TOBF1 modulates AS transcripts necessary for maintaining identity mouse ESCs. Among genes affected...
Locus-specific interrogation of target genes employing functional probes such as proteins and small molecules is paramount in decoding the molecular basis gene function designing tools to modulate its downstream effects. In this context, CRISPR-based editing targeting technologies have proved tremendously useful, they can be programmed any interest by simply changing sequence single guide RNA (sgRNA). Although these are widely utilized recruiting genetically encoded proteins, display using...
SUMMARY Genome editing using the CRISPR Cas9 system has been used to manipulate eukaryotic DNA and make precise heritable changes. Although widely Streptococcus pyogenes (SpCas9) its engineered variants have efficiently harnessed for numerous gene-editing applications across different platforms, concerns remain, regarding their putative off targeting at multiple loci genome. Here we report that Francisella novicida (FnCas9) shows a very high specificity of binding intended targets negligible...
Abstract Embryonic stem (ES) cells retain the ability to undergo lineage-specific differentiation that can eventually give rise different cell types constitute an organism. Although specific biological networks of transcription factors and epigenetic modifiers are well established, how ES transcriptional alternative splicing (AS) machinery regulate their expression has not been sufficiently explored. In this study, we show lncRNA associated protein TOBF1 regulates co-transcriptional...
Abstract Locus-specific interrogation of the genome using programmable CRISPR-based technologies is tremendously useful in dissecting molecular basis target gene function and modulating its downstream output. Although these tools are widely utilized recruiting genetically encoded functional proteins, display small molecules this technique not well developed due to inadequate labeling technologies. Here, we report development a modular technology, sgRNA-Click (sgR-CLK), which harnesses power...