T lymphocytes have been implicated in the onset of many autoimmune diseases; however, mechanisms underlying T-cell activation toward self antigens are poorly understood. To study whether T-lymphocyte costimulation can overcome immunologic unresponsiveness observed an vivo model, we created transgenic mice expressing costimulatory mouse molecule B7-1, a ligand for CD28 receptor, on pancreatic beta cells. We now report that triple-transgenic both B7-1 and viral glycoprotein their cells, along...

Endogenous mannary gland peroxidase in acinar cells of prelactating and lactating rats is revealed tannic acid-formaldehyde-glutaraldehyde-fixed tissue by means the standard diaminobenzidine procedure. Diaminobenzidine cytochemical reaction product present perinuclear cisternae, granular endoplasmic reticulum Golgi apparatus functionally differentiated secretory cells. The mammary thought to represent lactoperoxidase. Peroxidase staining diminished or absent hypophysectomized ovariectomized...

To assess the effect of computer-aided detection (CAD) brain metastasis (BM) on radiologists' diagnostic performance in interpreting three-dimensional magnetic resonance (MR) imaging using follow-up and consensus as reference standard.The institutional review board approved this retrospective study. The study cohort consisted 110 consecutive patients with BM 30 without BM. training data set included MR images 80 450 nodules. test 134 nodules We developed a CAD system for template-matching...

The garment-to-person virtual try-on (VTON) task, which aims to generate fitting images of a person wearing reference garment, has made significant strides. However, obtaining standard garment is often more challenging than using the already worn by person. To improve ease use, we propose MFP-VTON, Mask-Free framework for Person-to-Person VTON. Recognizing scarcity person-to-person data, adapt model and dataset construct specialized this task. Our approach builds upon pretrained diffusion...

Tissue biopsies and peripheral blood samples from 10 patients with the characteristic clinical features of acquired immunodeficiency syndrome (AIDS) were examined by electron microscopy ultracytochemical myeloperoxidase technique. Abundant tubuloreticular inclusions (TRI) detected within endoplasmic reticulum capillary endothelial cells, histiocytes, lymphocytes in kidneys, small bowel, lymph nodes, monocytes blood. In general, TRI found same type cells conspicuous high frequency our cases...

The estrogen antagonist C1628 maintains sustained hypertrophy of the uterine epithelium and synthesis many proteins including peroxidase. is a progestogen, inducing secretion protein by surface epithelial glandular cells. connective tissue mitogen, DNA in fibroblasts endothelium. share these properties mentioned above. Estrogen, however, induced moderate growth mucosa within 24-h period massive hyperplasia thereafter. alone, or combination with estradiol, does not have mitogenic effect on...

We investigated if the monocytic Ehrlichia are totally dependent on their host cells for energy, or, as Rickettsia, capable of some ATP synthesis in vitro. The Miyayama strain sennetsu and Maryland Illinois strains risticii were cultivated a mouse macrophage cell line, separated from constituents by procedures that included Renografin or Percoll gradient centrifugation, tested after cryopreservation. Cells incubated without metabolizing substrate contained little, any, ATP. When 1 hr at 34...

The in vitro metabolic activities of two monocytic species Ehrlichia were investigated. Miyayama strain sennetsu and strains risticii, isolated Illinois Maryland, cultivated a P388D1 mouse macrophage cell line. ehrlichia particles from heavily infected cultures separated host constituents by Renografin gradient centrifugation procedure modified those employed for rickettsiae chlamydiae. the ehrlichiae measured their formation CO2 after incubation 1 h or longer at 34 degrees C with...

The two endonucleases, Rad27 (yeast Fen1) and Dna2, jointly participate in the processing of Okazaki fragments yeasts. Mus81–Mms4 is a structure-specific endonuclease that can resolve stalled replication forks as well toxic recombination intermediates. In this study, we show suppress dna2 mutational defects by virtue its functional physical interaction with Rad27. stimulated activity significantly, accounting for ability to restore growth caused mutation. Interestingly, rate catalyzed...

Estrogens are known to increase cyclic guanosine monophosphate (cGMP) levels in the uterus of rats by enhancing guanylate cyclase (GC) activity. In present study, cytochemical localization GC activity was studied uteri immature and ovariectomized after treatment with diethylstilbestrol (DES), progesterone, estrogen antagonist (CI628), a combination DES CI628. Twenty-four hours first dose DES, moderate strong indicated lead phosphate precipitate on luminal microvillar basolateral surfaces...

Some structural features of the surface and interior granulosa cells from porcine ovarian follicles were examined at different developmental stages by scanning transmission electron microscopy. The presence numerous microvilli on luminal large follicles, their absence small medium noted. microfilaments within was also possible relationship between these appearance trophic hormone receptors is discussed.

The purpose of this study was to qualitatively and quantitatively evaluate the effects a metal artifact reduction for orthopedic implants (O-MAR) brain computed tomographic angiography (CTA) in patients with aneurysm clips coils.The included 36 consecutive 47 intracranial metallic (42 clips, 5 coils) who underwent CTA. images without O-MAR were independently reviewed both by two reviewers. For quantitative analysis, image noises near non-O-MAR compared. qualitative quality improvement...

The interactions of osteoblasts with one another and the extracellular milieu are vital importance for cell function. These mediated by membrane-associated components. In present work, we studied distribution several mediators known to be associated surface, using ultrastructural cytochemistry, characterize three membrane domains (osteoid, lateral, vascular) osteoblasts. Osteoblasts in neonatal rat calvariae were surface alkaline phosphatase (APase), calcium-activated adenosine...

Objective: To evaluate texture data of the torn supraspinatus tendon (SST) on preoperative T2-weighted magnetic resonance arthrography (MRA) using gray-level co-occurrence matrix (GLCM) for prediction post-operative state.Materials and Methods: Fifty patients who underwent arthroscopic rotator cuff repair full-thickness tears SST were included in this retrospective study.Based 1-year follow-up, imaging showed that 30 had intact SSTs, 20 retears.Using GLCM, two radiologists measured...

Bacterial lipopolysaccharide (LPS) is known to be implicated in the pathogenesis of endotoxemia and septic shock. The liver first vital organ exhibit pathological alterations present studies include immunoelectron microscopic localization tissue fibronectin cytochemical calcium enzymes hepatocytes animals with LPS-induced or cecal ligation-induced results showed increased staining basal (perisinusoidal) surfaces cisternae rough endoplasmic reticulum Golgi complex rats Intracellular content...

We describe a procedure for lipopolysaccharide (LPS) biotinylation using N-biotinyl-L-lysine and application of the biotinylated LPS (Bi-LPS) to localization binding sites subcellular distribution. Biotinylation was confirmed by enzyme-linked immunosorbent assay (ELISA), gel immunodiffusion, immunodot techniques. The biological toxicological activity Bi-LPS tested Limulus amoebocyte lysate (LAL) assays histopathological examinations, respectively. Results showed that biotin conjugated...

AIM: To report CT and MR imaging findings of ocular adnexal mucosa-associated lymphoid tissue lymphoma associated with IgG4-related disease (IgG4-MALT lymphoma), a rare but clinically important complication disease. METHODS: We retrospectively reviewed all cases histologically confirmed at three tertiary one secondary referral centers, between February 2003 December 2016. Seven histopathologically diagnosed IgG4-MALT were identified. images analyzed by consensus two experienced head neck...

High-resolution vessel wall MR imaging is prone to slow-flow artifacts, particularly when gadolinium shortens the T1 relaxation time of blood. This study aimed determine optimal preparation pulses for contrast-enhanced high-resolution imaging.Fifty patients who underwent both motion-sensitized driven equilibrium and delay alternating with nutation tailored excitation (DANTE) 3D-T1-FSE were retrospectively included. Qualitative analysis was performed using a 4-grade visual scoring system...

Objective: To evaluate pharmacokinetic variables from contrast-enhancing lesions (CELs) and non-enhancing T2 high signal intensity (NE-T2HSILs) on dynamic contrast-enhanced (DCE) magnetic resonance (MR) imaging for predicting progression-free survival (PFS) in glioblastoma (GBM) patients.Materials Methods: Sixty-four GBM patients who had undergone preoperative DCE MR received standard treatment were retrospectively included.We analyzed the of volume transfer constant (Ktrans) fraction...

<h3>BACKGROUND AND PURPOSE:</h3> Contrast-enhanced 3D-turbo spin-echo (TSE) black-blood sequence has gained attention, as it suppresses signals from vessels and provides an increased contrast-noise ratio. The purpose was to investigate which among the contrast-enhanced 3D T1 TSE, fast-spoiled gradient echo (FSPGR), T2 FLAIR sequences can better detect cranial nerve contrast enhancement. <h3>MATERIALS METHODS:</h3> Patients with neuritis based on clinical findings (<i>n</i> = 20) control...