A5090779981- Protein purification and stability
- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Nanopore and Nanochannel Transport Studies
- Protein Structure and Dynamics
- Ion-surface interactions and analysis
- Bacterial Genetics and Biotechnology
- Acoustic Wave Resonator Technologies
- Nuclear Physics and Applications
- Microfluidic and Bio-sensing Technologies
- Interconnection Networks and Systems
- Probabilistic and Robust Engineering Design
- Molecular Junctions and Nanostructures
- Advanced biosensing and bioanalysis techniques
- Biochemical and Molecular Research
- Computational Drug Discovery Methods
- Monoclonal and Polyclonal Antibodies Research
- Distributed and Parallel Computing Systems
Forschungszentrum Jülich
2017-2023
RWTH Aachen University
2021-2023
Amgen (Germany)
2023
Amgen (United States)
2021
University of Colorado Boulder
2014
Least squares estimation of unknown parameters from measurement data is a well-established standard method in chromatography modeling but can suffer critical disadvantages. The description real-world systems generally prone to unaccounted mechanisms, such as dispersion external holdup volumes, and systematic errors, caused by pump delays. In this scenario, matching the shape between simulated measured chromatograms has been found be more important than exact peak positions. We have therefore...
We have developed a simple system for tagging and purifying proteins. Recent experiments demonstrated that RTX (Repeat in Toxin) motifs from the adenylate cyclase toxin gene (CyaA) of B. pertussis undergo conformational change upon binding calcium, resulting precipitation fused proteins making this method viable alternative bioseparation. designed an iGEM Biobrick comprised tag can be easily to any protein interest. In paper, we detail process creating tagged version restriction enzyme EcoRI...
Chromatography equipment includes hold-up volumes that are external to the packed bed and usually not considered in development of chromatography models. These can substantially contribute band-broadening system deteriorate predicted performance. We selected a bubble trap pilot scale as an example for volume with non-standard mixing behavior. In worst-case scenario, is properly flushed before elution, thus causing significant elution peak. showed buffers different densities device be...