Circular RNAs are key regulators in human cancers, however, there is a lack of studies on circRNAs' specific functions ovarian cancer.Our study used qRT-PCR to detect the differentially expressed circRNAs between normal ovaries and cancer tissues. Cell function experiments were performed verify role overexpression silence circWHSC1, including MTT assay, cell apoptosis wound healing Matrigel-coated Transwell assay. In vivo tumorigenesis model was constructed by subcutaneous injection nude...

There is increasing evidence in support of the role lncRNAs tumor cell proliferation, differentiation and apoptosis.We examined expression lncRNA ABHD11-AS1 epithelial ovarian cancer (EOC) tissues normal by real-time quantitative PCR (qRT-PCR). After inducing downregulation small interfering RNA (siRNA) or overexpression plasmid transfection, we EOC phenotypes related molecules.Expression was higher than that tissue. It positively associated with stage (stage I/II vs. III/IV), it lower...

Highly upregulated in liver cancer (HULC) is a long noncoding RNA (lncRNA), which has recently been identified as key regulator the progression of hepatocellular carcinoma, gliomas and gastric cancer. However, its role epithelial ovarian carcinoma (EOC) remains unknown. In this study, HULC expression was examined EOC, borderline benign tumors, normal tissues by RT-PCR. Ovarian cell phenotypes, well autophagy-associated proteins were after overexpression or downregulation plasmid small...

Maternally expressed gene 3 (Meg3), a long non-coding RNA, has been reported to be associated with the pathogenesis of multiple malignancies. However, little is known regarding role Meg3 in epithelial ovarian cancer (EOC). In this study, we found that expression was lower carcinoma, and potential considered as biomarker for cancer. After transfecting cell lines OVCAR3 A2780 Meg3, phenotypic changes autophagy-related molecules were examined. Upregulation inhibited proliferation, plate colony...

Circular RNAs (circRNAs) have been reported to participate in the molecular mechanism of human cancers. The PUM1 gene has confirmed be closely related tumorigenesis and progression ovarian cancer. In present study, we explored function underlying circPUM1 qRT-PCR analysis showed upregulation cancer tissues compared with normal ovaries. Gain- loss-of-function experiments indicated that increased cell proliferation, migration, invasion inhibited apoptosis. Intraperitoneal injection...

Circular RNAs (circRNAs) play important roles in human cancer progression. Their high stability and tissue specificity make circRNAs molecular targets for clinical diagnosis, treatment prognosis. However, the functions mechanisms of circRNA WHSC1 endometrial are unknown. CircWHSC1 expression normal tissues was detected using PCR. Overexpression or knockdown circWHSC1 cell lines HEC-1B Ishikawa, respectively, function experiments were used to detect impact on cells. A nude mouse xenograft...

Endometrial carcinoma (EC) is one of the most lethal gynecologic cancers. Patients frequently have regional or distant metastasis at diagnosis. MicroRNAs are small non-coding RNAs that participate in numerous biological processes. Recent studies demonstrated miR-505 associated with several types cancer; however, expression and function not been investigated EC. normal endometrial tissue, carcinomas were quantified by Quantitative reverse transcription PCR. The cell lines HEC-1B Ishikawa each...

Background: Accumulating studies reveal that aberrant microRNA (miRNA) expression can affect the development of chemotherapy drug resistance by modulating relevant target proteins. The aim this study was to investigate role miR-133b in ovarian cancer cells. Methods: We examined levels carcinoma tissues and human cell lines (A2780, A2780/DDP A2780/Taxol, respectively). determined viability these treated with cisplatin or paclitaxel presence absence anti-miR-133b transfection using MTT assay....

An increasing amount of evidence has revealed that microRNAs regulate various biological processes, including cell differentiation, proliferation, apoptosis, drug resistance, and fat metabolism. Studies have shown miR-93's targetome in cancer not been fully defined. Moreover, the role miR-93 epithelial ovarian carcinoma (EOC) remains largely unknown.MIR-93 mRNA expression normal tissue, benign tumors, borderline primary carcinomas, metastatic omentum was quantified. The lines OVCAR3,...

Abstract Recently, a large number of studies have focused on the important role long non‐coding RNA s (lnc s) in metabolism and development found that abnormal lnc expression is associated with pathogenesis many diseases. The DLEU 1 involved solid tumours haematological malignancies. However, its epithelial ovarian carcinoma ( EOC ) molecular mechanisms has not been reported. In this study, quantitative reverse transcription– PCR qRT – demonstrated higher tissues than normal tissues. Plasmid...

Recent studies have shown that microRNAs may regulate the ABCB1 gene (ATP-binding cassette, sub-family B [MDR/TAP], member 1). Computational programs predicted 3'-untranslated region (3'-UTR) of contains a potential miRNA-binding site for miR-186. Here, we investigated role miR-186 in sensitizing ovarian cancer cells to paclitaxel and cisplatin.Human carcinoma cell lines OVCAR3, A2780, A2780/DDP, A2780/Taxol were exposed or cisplatin with without transfection, viability was determined by MTT...

Circular RNAs are known to participate in tumorigenesis through a variety of pathways, and as such, have potential serve molecular markers tumor diagnosis treatment. Here, using quantitative reverse transcription (qRT)-PCR, we showed that circ-CSPP1 is highly expressed ovarian cancer (OC) tissues. Particularly, detected expression three OC cell lines; which, OVCAR3 A2780 demonstrated higher levels expression, CAOV3 lower level. Subsequent silencing lines revealed decreased growth, migration...

CEMIP is a cell migration-inducing protein that closely associated with carcinogenesis. However, the function of has not been reported in ovarian cancer. Here we show expression level cancer tissues was higher than normal ovaries. Silencing lines A2780 and OVCAR3 significantly decreased proliferation, migration invasion capacity, while proportion apoptotic cells increased. In addition, Western blotting demonstrated knockdown inhibited activation PI3K/AKT signaling pathway. Altogether, these...

Endometrial cancer is one of the most common gynaecological malignancies and sixth cause cancer-related death among women. Here, we define role molecular mechanism circ_0000043 (hereafter referred to as circ_PUM1) in development progression endometrial carcinoma. QRT-PCR was used detect expression circ_PUM1 normal tissue carcinoma tissues. Changes cell function tumorigenicity nude mice were examined after overexpression or knockdown. Bioinformatic analysis dual-luciferase reporter assay...

Abstract Circular RNAs (circRNAs) play important roles in cancer tumorigenesis and progression, representing prognostic biomarkers therapeutic targets. In this case, we demonstrated the role of circ-NOLC1 epithelial ovarian (EOC). Our results have shown that Circ-NOLC1 expression was higher EOC tissues than normal tissues, positively associated with FIGO stage, differentiation. Among cell lines, highest A2780, lowest CAOV3. Overexpression CAOV3 cells increased proliferation, migration,...

Peripheral nervous system (PNS) injuries often lead to significant sensory and motor impairments. Traditional artificial nerve conduits, lacking anisotropic structures, have been associated with prolonged repair time failures in regeneration. This study aimed address these challenges by developing a novel approach for rapid of peripheral (PNI). A 3D oriented fibers scaffold featuring distinct radial (RFs) longitudinal (LFs) orientations was engineered using coaxial electrospinning gas...

Breast cancer ranks as one of the most prevalent malignancies among women globally, with increasing incidence rates. Physical activity, particularly exercise, has emerged a potentially significant modifier prognosis, influencing tumor biology and patient outcomes.

Prostate cancer gene expression marker 1 (PCGEM1) is a long noncoding RNA (lncRNA) and well known as promoter in prostate osteoarthritis synoviocytes. However, the role PCGEM1 plays epithelial ovarian unknown.PCGEM1 was examined normal tissues using reverse transcription-PCR. Ovarian cell phenotypes genotypes were after overexpression or downregulation vitro; besides, effects of also vivo.PCGEM1 level higher than positively associated with differentiation (Well vs. Mod/Poor). Upregulation...

The small nucleolar RNA (snoRNA) is a type of non-coding widely distributed in the nucleoli eukaryotic cells, promoting cancer development. aim this study was to assess box C/D snoRNA 89 (SNORD89) dysregulations endometrial cancer. According TCGA database as well International Federation Gynecology and Obstetrics (FIGO), higher SNORD89 expression found tissues. In addition, level carcinoma with lymph node metastasis than without metastasis. By interacting conservative chaperone protein...

Abstract Ovarian cancer (OC) is a heterogeneous cancer. In contrast to other tumor cells, which rely primarily on aerobic glycolysis (Warburg effect) as their energy source, oxidative phosphorylation (OXPHOS) also one of its major metabolic modes. Piwi-interacting RNAs (piRNAs) play regulatory function in various biological processes cells. However, the role and mechanisms piRNAs OC mitochondrial OXPHOS remain be elucidated. Here, we found that piR-26441 was aberrantly downregulated OC,...

Conventional targeted therapies primarily rely on small molecules and proteins. However, they often struggle to enter specific protein conformations, which limits their therapeutic effectiveness. In contrast, RNA therapy is emerging as a more precise effective targeting strategy. Through differential analysis comparing Pandora-Seq data with clinical samples, we discovered that the piR-28846 naturally, occurring in human body, exhibited low expression levels ovarian cancer tissues....