A5103236744- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Cancer Mechanisms and Therapy
- Endometrial and Cervical Cancer Treatments
- Cancer Genomics and Diagnostics
- Ovarian cancer diagnosis and treatment
- Epigenetics and DNA Methylation
- Mechanisms of cancer metastasis
- Cancer-related cognitive impairment studies
- Cancer-related gene regulation
- Dementia and Cognitive Impairment Research
- RNA Research and Splicing
- Animal Genetics and Reproduction
- Ferroptosis and cancer prognosis
- Virus-based gene therapy research
- Glioma Diagnosis and Treatment
- Bone and Dental Protein Studies
- Genetic factors in colorectal cancer
- Angiogenesis and VEGF in Cancer
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Ovarian function and disorders
- Genomics, phytochemicals, and oxidative stress
Third Affiliated Hospital of Guangzhou Medical University
2016-2025
Guangzhou Medical University
2016-2025
Target (United States)
2024
Key Laboratory of Guangdong Province
2024
Gynecologic Oncology Group
2024
Wenzhou Medical University
2011
China Medical University
2007
First Hospital of China Medical University
2007
The aims of this study were to investigate the functions GAS5 as a tumor suppressor in cervical cancer and explore mechanism.The expression microRNA 21 (miR-21) was detected primary tissue specimens, well cell lines. We identified interaction miR-21 by quantitative polymerase chain reaction, Western blot, dual-luciferase reporter assay. also studied proliferation, apoptosis, migration, invasion cells vitro vivo. Finally, impact on cisplatin resistance its mechanism identified.The GAS5, which...
Metastasis associated in lung adenocarcinoma transcript-1 (MALAT-1) is overexpressed during cancer progression and promotes cell migration invasion many solid tumors. However, its role ovarian remains poorly understood.Expressions of MALAT-1 were detected 37 normal tissues 45 by reverse transcription polymerase chain reaction (RT-PCR). Cell proliferation was observed CCK-8 assay; Flow cytometry used to measure cycle apoptosis; transwell assay. In order evaluate the function MALAT-1, shRNA...
Ovarian cancer is the most common and lethal gynecological tumor in women worldwide. High-grade serous ovarian carcinoma (HGSOC) one of histological subtypes epithelial cancer, accounting for 70%. It often occurs at later stages associated with a more fatal prognosis than endometrioid carcinomas (EC), another subtype cancer. However, molecular mechanism biology underlying metastatic HGSOC (HG_M) immunophenotype remain poorly elusive. Here, we performed single-cell RNA sequencing analyses...
Conventional targeted therapies primarily rely on small molecules and proteins. However, they often struggle to enter specific protein conformations, which limits their therapeutic effectiveness. In contrast, RNA therapy is emerging as a more precise effective targeting strategy. Through differential analysis comparing Pandora-Seq data with clinical samples, we discovered that the piR-28846 naturally, occurring in human body, exhibited low expression levels ovarian cancer tissues....
Charged multivesicular body protein 4C (CHMP4C), as a subunit of endosomal sorting complex required for transport-III, is important the abscission checkpoint in cell division, preventing premature division and genetic damage. The present study aimed to assess role CHMP4C cervical cancer associated mechanisms. levels normal tissues were detected by immunohistochemistry. MTT assay, apoptosis, wound-healing invasion assay performed. Western blotting was performed analyze level cancer-related...
Abstract Maspin (SerpinB5) is an epithelial-specific tumor suppressor gene product that displays context-dependent cellular functions. Maspin-deficient mouse models created to date have not definitively established maspin functions critical for cancer suppression. In this study, we generated a strain in which exon 4 of the was deleted, confirming its essential role development but also enabling breeding scheme bypass embryonic lethality. Phenotypic characterization viable deficiency...
Endometrial cancer is the most common gynaecological malignancy encountered in developed countries and second developing world. The five-year survival rate of patients with endometrial diagnosed at a late stage <30%. Therefore, it critical to develop suitable chemotherapeutic regimen for late-stage cancer. Cisplatin (CDDP) first-line drug chemotherapy. present study investigated molecular mechanism underlying effect CDDP on from perspective cell autophagy. Ishikawa cells were treated 10, 20,...
Subjective cognitive impairment can be a significant and prevalent problem for gynaecological cancer survivors. The aims of this study were to assess subjective functioning in survivors after primary treatment, investigate the impact treatment on brain structural networks its association with impairment.This was cross-sectional survey using self-reported questionnaire by Functional Assessment Cancer Therapy-Cognitive Function (FACT-Cog) functioning, applying DTI (diffusion tensor imaging)...
Background: Among women in China, gynecological cancers are the second most common after breast cancer. Cancer-related cognitive impairment (CRCI) has emerged as a significant problem affecting cancer survivors. While acupuncture been used different aspects of care, possible positive effects on have received little attention. This study hypothesized that patients would demonstrate lower neurocognitive performance and structural connectivity compared to healthy controls. pilot also may...
Epithelial ovarian carcinoma (EOC) is one of the most common gynecologic malignancies with a high mortality rate. Serum biomarkers and imaging approaches are insufficient in identifying EOC patients at an early stage. This study to set up combination proteins from serum small extracellular vesicles (sEVs) for diagnosis early-stage determine its performance. A biomarker cancer (BESOC) cohort was used as Chinese multi-center population-based registered Clinical Trial ChiCTR2000040136. The sEV...
Previous neurocognitive assessments in non-central nervous system cancers highlight the high incidence of dysfunction this study population. However, there have been few studies exploring induced by chemotherapy gynecological cancer patients. This prospective longitudinal was conducted to assess functioning and functional brain networks Chinese patients pre- post-chemotherapy, while additionally including age-matched healthy subjects as control group.All research participants were evaluated...
Understanding germline and somatic status in patients with gynecologic cancers could improve risk assessment guide therapeutic decision-making.
SRY-box containing gene 17 (SOX17) plays a pivotal role in cancer onset and progression is considered potential target for diagnosis treatment. However, the expression pattern of SOX17 its clinical relevance remains unknown. Here, we explored relationship between drug response by examining patterns across multiple types.Single-cell bulk RNA-seq analyses were used to explore profile SOX17. Analysis results verified with qPCR immunohistochemistry. Survival, response, co-expression performed...
Apoptosis induction by short hairpin RNA (shRNA) expression vectors may be an efficient and promising strategy for cancer gene therapy. Ultrasound-targeted microbubble destruction (UTMD) is appealing technique; however, there few data are available to demonstrate the feasibility optimize methodology this technology. The aim of study was technique elucidate effects on inhibition apoptosis in vitro. Human cervical cell lines were obtained cultured.shRNA constructed, UTMD examined determine...
Lipase G, endothelial type (LIPG) is expressed abundantly in tissues with a high metabolic rate and vascularisation. Research on LIPG has focussed syndromes. However, the role of providing lipid precursors suggests that it might function metabolism carcinoma cells. Analysis The Cancer Genome Atlas indicated patients cervical expression had lower survival prognosis compared low expression. mechanism underlying effects unclear. present study aimed to determine its mechanism. results showed...
The metastatic or recurrent potential of localized human papillomavirus-associated endocervical adenocarcinoma (HPVA EAC) is difficult to predict, especially based upon biopsy alone. Recent analyses small cohorts indicate that high tumor nuclear grade (TNG) and the presence necrotic debris (NTD) from HPVA EACs in cervical specimens are highly predictive nodal metastasis (NM). In present study, we aimed investigate how reliably tumoral morphologic features predict NM recurrence (TR) patient...
Ovarian tumors do not really typically occur in association with pregnant; however, once they do, the treatment is critical. It important to note that around 6% of ovarian pregnancies are cancerous. The problems induced by pregnancy particularly necessitate rapid medical intervention and much more frequent than cancer. Medication choices survival ovary tumor patients could be influenced varied diagnoses masses. So, we present an upgraded logistic regression (ULR) approach this paper....
Studies have shown that circRNAs play an important regulatory role in trophoblast function and embryonic development. Based on sequencing functional experiments, we found hsa_circ_0069443 can regulate the of cells, its presence is exosomes secreted by cells. It known mediate interaction between uterus embryo, which crucial for successful pregnancy. We cell-derived overexpressing promoted migration invasion endometrial stromal cells as well EMT process glandular epithelial this promotes...
Abstract Background Cervical cancer is the second most common gynaecological tumor of women worldwide, however, molecular mechanism for cervical carcinogenesis remains elusive. Current study provides a series genome-wide DNA methylation blueprints normal cervix and cancers using Whole Genome Bisulfite Sequencing. Results dynamic alternations during were focused on signal pathway TGF-beta epidermal growth factor, which could be used to monitor treatment response Tumorigenesis. Transcription...
Objective To study the effect of BRMS1 on invasion and metastasis ovarian cancer cells. Methods small interfering RNA (BRMS1-siRNA) was transfected into human cell-line OVCAR3 by liposome transfection method. The cells were divided 3 groups: experimental group with BRMSl-siRNA, negative control siRNA that did not impact any gene, blank without transfection. Changes migration in per-formed using transwell assay. Results gene silenced cell line successfully detecting quantitative real-time...