A5044949253- Nanoplatforms for cancer theranostics
- Photodynamic Therapy Research Studies
- Nonmelanoma Skin Cancer Studies
- Cutaneous Melanoma Detection and Management
- Prostate Cancer Treatment and Research
- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- Advanced Radiotherapy Techniques
- Prostate Cancer Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Cancer and Skin Lesions
- Cancer Cells and Metastasis
- Bone and Dental Protein Studies
- Radiation Dose and Imaging
- Bone Metabolism and Diseases
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cutaneous lymphoproliferative disorders research
- Cancer, Stress, Anesthesia, and Immune Response
- Luminescence Properties of Advanced Materials
- Medical Imaging Techniques and Applications
- Corneal Surgery and Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Radiopharmaceutical Chemistry and Applications
- Veterinary Oncology Research
- Cell death mechanisms and regulation
Case Western Reserve University
2014-2024
Case Comprehensive Cancer Center
2021
Cancer Clinic
2005
Diosmetin, a plant flavonoid, has been shown to exert promising effects on prostate cancer cells as an anti‑proliferative and anticancer agent. In this study, using western blot analysis for protein expression flow cytometry cell cycle analysis, we determined that the treatment of LNCaP PC‑3 with diosmetin resulted in marked decrease cyclin D1, Cdk2 Cdk4 levels (these proteins remain active G0‑G1 phases cycle). These changes were accompanied by c-Myc Bcl-2 expression, increase Bax, p27Kip1...
Metastatic castration-resistant prostate cancer poses a serious clinical problem with poor outcomes and remains deadly disease. New targeted treatment options are urgently needed. PSMA is highly expressed in has been an attractive biomarker for the of cancer. In this study, we explored feasibility delivery antimitotic drug, monomethyl auristatin E (MMAE), to tumor tissue using small-molecule based lig-and. With aid Cy5.5, found that cleavable linker vital antitumor activity ligand-drug...
Rationale: Although surgery and radiation therapy in patients with low risk prostate cancer appear appropriate effective, those high-risk localized disease almost always become hormone refractory then rapidly progress.A new treatment strategy is urgently needed for cancer, particularly an approach that combines two drugs different mechanisms.Combinations of photodynamic (PDT) chemotherapy have shown synergistic effects clinical trials, but are limited by off-target toxicity.Prostate specific...
Keratinocyte carcinomas, including basal and squamous cell are the most common human cancers worldwide. While 75% of all keratinocyte carcinoma (4 million annual cases in United States) treated with conventional excision, this surgical modality has much lower cure rates than Mohs micrographic surgery, likely due to bread-loaf histopathologic assessment that visualizes <1% tissue margins. A quenched protease-activated fluorescent probe 6qcNIR, which produces a signal only protease-rich tumor...
In an effort to increase the efficiency and cure rate of nonmelanoma skin cancer (NMSC) excisions, we have developed a point-of-care method imaging evaluation margins. We evaluate surgical specimens using smart, near-infrared probe (6qcNIR) that fluoresces in presence cathepsin proteases overexpressed NMSC. Imaging is done with inverted, flying-spot fluorescence scanner reduces scatter, giving 70% improved step response as compared conventional system. develop scheme for careful comparison...
Abstract Background Male dogs can develop spontaneous prostate cancer, which is similar physiologically to human disease. Recently, Tweedle and coworkers have developed an orthotopic canine model allowing implanted tumors therapeutic agents be tested in a more translational large animal model. We used the evaluate prostate‐specific membrane antigen (PSMA)‐targeted gold nanoparticles as theranostic approach for fluorescence (FL) imaging photodynamic therapy (PDT) of early stage cancer....
Abstract Background Prostate cancer (PCa) models in mice and rats are limited by their size lack of a clearly delineated or easily accessible prostate gland. The canine PCa model is currently the only large animal which can be used to test new preclinical interventions but costly availability sparse. As an alternative, we developed orthotopic human tumor immunosuppressed New Zealand White rabbit. Rabbits phylogenetically closer humans, gland anatomically similar, its allows for...
Background: Tumor-positive surgical margins during primary breast cancer (BCa) surgery are associated with a two-fold increase in the risk of local recurrence when compared tumor-negative margins. Pathological microscopic evaluation samples only assesses about 1/10 1% entire volume removed BCa specimens, leading to margin under-sampling and potential patients pathologically clean margins, i.e., false negative In case tumor-positive need undergo re-excision and/or radiation therapy, resulting...
Abstract Introduction: Prostate cancer is the most prevalent among men in United States and their second leading cause of death. During initial screen, about 17-31% patients are diagnosed with non-metastatic high-risk localized prostate cancer. These have an increased risk treatment failure oncological progression. The contemporary paradigm high moving toward a multidisciplinary integrated approach systemic local therapy. In addition, incorporation molecular biomarkers can also improve...
Abstract Purpose Prostate specific membrane antigen (PSMA) has been studied in human breast cancer (BCa) biopsies, however, lack of data on PSMA expression mouse models impedes development PSMA-targeted therapies, particularly improving conserving surgery (BCS) margins. This study aimed to validate and characterize the murine BCa models, demonstrating that can be utilized improve therapies imaging techniques. Methods Murine triple negative 4T1 cells, cell lines, MDA-MB-231, MDA-MB-468,...
Prostate cancer is the most prevalent among men in United States and a leading cause of cancer-related death. specific membrane antigen (PSMA) has been established as biomarker for prostate diagnosis treatment. This study aimed to develop novel theranostic agent, PSMA-1-MMAE-Pc413, which integrates PSMA-targeting ligand, photosensitizer Pc413, microtubular inhibitor monomethyl auristatin E (MMAE) synergistic therapeutic efficacy. In vitro uptake studies revealed that PSMA-1-MMAE-Pc413...
Up to 40 % of breast cancer patients have a tumor-positive resection margin (TPRM) - defined as cells at the surface resected specimen after breast-conserving surgery (BCS), necessitating re-resection or boost radiation. To prevent these additional treatments, intraoperative near-infrared (NIR) fluorescence imaging with topically applied, cathepsin-activatable agent AKRO-6qcICG might be used detect TPRMs and guide resection. Here, validate its performance, is applied all surfaces freshly...
Prostate and breast cancer are the most prevalent primary malignant human tumors globally. Prostatectomy conservative surgery remain common definitive treatment option for >500,000 men women newly diagnosed with localized prostate each year only in US. Morphological examination is mainstay of diagnosis but margin under-sampling excised tissue may lead to local recurrence. In despite progress non-invasive optical imaging, there still a clinical need targeted imaging probes that could rapidly...
The loading of needles for loose seed implantation the prostate gland results requires a significant amount effort and some radiation exposure to members medical staff. This study was performed quantify time spent levels associated with implant preparation, as well investigate any improvement in or burden due introduction new device. movements exposures two single, highly experienced dosimetrists were monitored ten conventionally loaded iodine cases. These same cases reloaded dummy sources...
We have developed a point-of-care imaging method for non-melanoma skin cancer surgery whereby excised tissues are imaged with smart near infrared quenched protease probe (6qcNIR) that fluoresces in the presence of overexpressed cathepsin proteases basal cell carcinoma (BCC) and squamous (SCC), determine if margins clear cancer. Here we report our system to validate detection samples an inverted, flying spot fluorescence scanner (LI-COR Odyssey CLx). Scatter was greatly reduced giving 80%...
Abstract Of the non-melanoma skin cancers, 75% are basal cell carcinomas (BCC) and rest is squamous (SCC). They most common cancers in humans, their incidence may soon surpass that of all other cancers. Four million people will be diagnosed with BCC/SCC this year US overall workload diagnosis management increasing by 50% 2030. Mohs micrographic surgery (MMS), which examines margins entire resected sample, treatment choice for BCC/SCC. However, ¾ cancer lesions too large to practically...