A5012477004- Neuroscience and Neuropharmacology Research
- Calpain Protease Function and Regulation
- Memory and Neural Mechanisms
- Photoreceptor and optogenetics research
- Mitochondrial Function and Pathology
- Ion channel regulation and function
- Neural dynamics and brain function
- Neuroinflammation and Neurodegeneration Mechanisms
- Receptor Mechanisms and Signaling
- Lipid Membrane Structure and Behavior
- Amino Acid Enzymes and Metabolism
- Nerve injury and regeneration
- Polyamine Metabolism and Applications
- Connexins and lens biology
- Biochemical effects in animals
- RNA regulation and disease
- Molecular Sensors and Ion Detection
- Genetic Syndromes and Imprinting
- Estrogen and related hormone effects
- Cellular transport and secretion
- Alzheimer's disease research and treatments
- Neuroscience and Neural Engineering
- Metabolism and Genetic Disorders
- Stress Responses and Cortisol
- Carbohydrate Chemistry and Synthesis
Western University of Health Sciences
2015-2024
Graduate School USA
2021
Pomona College
2017
Rhenovia (France)
2008-2016
Western University
2015
University of Southern California
2004-2013
Southern California University for Professional Studies
1993-2012
Centre de Recherche en Neurosciences de Lyon
2012
LAC+USC Medical Center
1992-2009
Society for Neuroscience
2001-2008
Abstract Membranes from rat telencephalon contain a single class of strychnine‐insensitive glycine sites. That these sites are associated with N ‐methyl‐ d ‐aspartic acid (NMDA) receptors is indicated by the observations that [ 3 H]glycine binding selectively modulated NMDA receptor ligands and, conversely, several amino acids interacting increase H] ‐[1‐(2‐thienyl)cyclohexyl]piper‐idine ([ H]TCP) to phencyclidine site receptor. The endogenous compound kynurenate and related quinoline...
Conservation of normal cognitive functions relies on the proper performance nervous system at cellular and molecular level. The mammalian nicotinamide-adenine dinucleotide-dependent deacetylase SIRT1 impacts different processes potentially involved in maintenance brain integrity, such as chromatin remodeling, DNA repair, cell survival, neurogenesis. Here we show that is expressed neurons hippocampus, a key structure learning memory. Using combination behavioral electrophysiological...
Oxidative stress has been implicated in cognitive impairment both old experimental animals and aged humans. This implication led to the notion that antioxidant defense mechanisms brain are not sufficient prevent age-related increase oxidative damage dietary intake of a variety antioxidants might be beneficial for preserving function. Here we report dramatic loss learning memory function from 8 11 months age mice, associated with marked increases several markers stress. Chronic systemic...
The calcium-activated thiol-protease calpain I, which is present in cytosolic and membrane preparations from rat brain, was tested for its capacity to degrade the neuronal spectrin-like protein fodrin. In presence of micromolar calcium concentrations purified I degraded both fodrin hippocampal cerebellar membranes. Fodrin identified as a high molecular weight brain membranes by following criteria: (i) comigration on NaDodSO4/polyacrylamide gels with fodrin, (ii) reactivity antibodies (iii)...
Estrogen replacement therapy in women is associated with improvement of cognitive deficits and reduced incidence Alzheimer's disease. The present study indicates that estrogen neuroprotective against N -methyl- d -aspartate (NMDA)- kainate-mediated neurotoxicity, an effect mediated by tyrosine kinase/mitogen-activated protein kinase (MAPK) pathways. also stimulates phosphorylation NMDA receptors via src kinase/MAPK pathway. Finally, estrogen-mediated enhancement long-term potentiation...
The present study tested the effects of EUK-134, a synthetic superoxide dismutase/catalase mimetic, on several indices oxidative stress and neuropathology produced in rat limbic system as result seizure activity elicited by systemic kainic acid (KA) administration. Pretreatment rats with EUK-134 did not modify latency for or duration KA-induced activity. It produce highly significant reduction increased protein nitration, activator protein-1- NF-κB-binding activity, spectrin proteolysis well...
The p53 tumor-suppressor gene encodes a growth-regulatory protein that has been implicated in programmed cell death. To investigate the possible role of neuronal death, we studied expression associated with excitotoxicity adult rat brain. Within hours systemic administration glutamate analogue kainic acid, mRNA levels were increased neurons exhibiting morphological features damage within kainate-vulnerable brain regions. A similar distribution was found for DNA as evidenced by situ...
Oxygen free radicals have been proposed to mediate amyloid peptide (beta-AP)-induced neurotoxicity. To test this hypothesis, we evaluated the effects of EUK-8, a synthetic catalytic superoxide and hydrogen peroxide scavenger, on neuronal injury produced by beta-AP in organotypic hippocampal slice cultures. Cultures equivalent postnatal day 35 (defined as mature) 14 immature) were exposed various concentrations (1-42 or 1-40) absence presence 25 microM EUK-8 for up 72 hours. Neuronal was...
Brain-derived neurotrophic factor (BDNF) stimulates local dendritic mRNA translation and is involved in formation consolidation of memory. 2H,3H,6aH-pyrrolidino[2'',1''-3',2']1,3-oxazino[6',5'-5,4]-benzo[e]1,4-dioxan-10-one (CX614), one the best-studied positive AMPA receptor modulators (also known as ampakines), increases BDNF protein facilitates long-term potentiation (LTP) induction. Several other ampakines also improve performance various behavioral learning tasks. Since synthesis has...
Hippocampal synaptic structure and function exhibit marked variations during the estrus cycle of female rats. Estradiol activates mitogen-activated protein (MAP) kinase pathway in numerous cell types, MAP has been shown to play a critical role mechanisms underlying plasticity. Here, we report that endogenous estrogen produces tonic phosphorylation/activation extracellular signal-regulated 2 (ERK2)/MAP throughout rat brain an increase tyrosine phosphorylation NR2 subunits N -methyl- d...
Excitotoxicity is generally studied in dissociated neurons, cultured hippocampal slices, or intact animals. However, the requirements of neurons slices to use prenatal juvenile rats seriously limit advantages these systems, whereas complexity animals prevents detailed molecular investigations. In present experiments, we developmental changes NMDA neurotoxicity acute with lactate dehydrogenase (LDH) release medium, propidium iodide (PI) uptake, and Nissl staining as markers cell damage....
Inhalation of airborne particulate matter (PM) derived from urban traffic is associated with pathology in the arteries, heart, and lung; effects on brain are also indicated but less documented.We evaluated rodent responses to nanoscale (< 200 nm) PM (nPM).Ambient nPM collected near an freeway was transferred aqueous suspension reaerosolized for 10-week inhalation exposure mice or directly applied rat cell cultures.Free radicals were detected by electron paramagnetic resonance 30 days after...
Incubation of cortical synaptic membranes with low concentrations calcium resulted in a decrease the amount high-molecular-weight doublet protein and an increase sodium-independent binding glutamate. Both effects were blocked by thiol protease inhibitor leupeptin. These results suggest that calcium-induced proteolysis membrane components regulates number glutamate receptors neuronal membranes.