A5066748973- Neuroscience and Neuropharmacology Research
- Calpain Protease Function and Regulation
- Genetic Syndromes and Imprinting
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Nerve injury and regeneration
- Connexins and lens biology
- Genetics and Neurodevelopmental Disorders
- RNA regulation and disease
- Cellular transport and secretion
- Receptor Mechanisms and Signaling
- Epigenetics and DNA Methylation
- Lipid Membrane Structure and Behavior
- Neurogenesis and neuroplasticity mechanisms
- PI3K/AKT/mTOR signaling in cancer
- Venomous Animal Envenomation and Studies
- Lysosomal Storage Disorders Research
- Hedgehog Signaling Pathway Studies
- Mitochondrial Function and Pathology
- Biochemical effects in animals
- Photoreceptor and optogenetics research
- Cholinesterase and Neurodegenerative Diseases
- Traumatic Brain Injury and Neurovascular Disturbances
- Cancer-related Molecular Pathways
- Memory and Neural Mechanisms
Western University of Health Sciences
2015-2024
Chongqing University
2024
Changhai Hospital
2009-2019
Second Military Medical University
2009-2019
Pomona College
2018
University of California, Irvine
1998-2005
University of Southern California
1996-2003
LAC+USC Medical Center
1994-1998
Oxidative stress has been implicated in cognitive impairment both old experimental animals and aged humans. This implication led to the notion that antioxidant defense mechanisms brain are not sufficient prevent age-related increase oxidative damage dietary intake of a variety antioxidants might be beneficial for preserving function. Here we report dramatic loss learning memory function from 8 11 months age mice, associated with marked increases several markers stress. Chronic systemic...
Brain-derived neurotrophic factor (BDNF) stimulates local dendritic mRNA translation and is involved in formation consolidation of memory. 2H,3H,6aH-pyrrolidino[2'',1''-3',2']1,3-oxazino[6',5'-5,4]-benzo[e]1,4-dioxan-10-one (CX614), one the best-studied positive AMPA receptor modulators (also known as ampakines), increases BDNF protein facilitates long-term potentiation (LTP) induction. Several other ampakines also improve performance various behavioral learning tasks. Since synthesis has...
It has been proposed that the endurance of long-term potentiation (LTP) depends on structural changes entailing reorganization spine actin cytoskeleton. The present study used a new technique involving intracellular and extracellular application rhodamine-phalloidin to conventional hippocampal slices test whether induction LTP by naturalistic patterns afferent activity selectively increases polymerization in juvenile young adult spines. Rhodamine-phalloidin, which binds polymerized actin,...
Prolonged calpain activation is widely recognized as a key component of neurodegeneration in variety pathological conditions. Numerous reports have also indicated that synaptic NMDA receptors (NMDARs) provides neuroprotection against insults. Here, we report the paradoxical finding such involves activation. NMDAR cultured rat cortical neurons was neuroprotective starvation and oxidative stress-induced damage. It resulted degradation two splice variants PH domain Leucine-rich repeat Protein...
Gated solely by activity-induced changes in intracellular calcium, small-conductance potassium channels (SKs) are critical for a variety of functions the CNS, from learning and memory to rhythmic activity sleep. While there is wealth information on SK2 gating, kinetics, Ca2+ sensitivity, little known regarding regulation subcellular localization. We report here that synaptic levels regulated E3 ubiquitin ligase UBE3A, whose deficiency results Angelman syndrome overexpression increased risk...
Deciphering and storing information coded in different firing patterns are important properties of neuronal networks, as they allow organisms to respond adapt external internal events. Here we report that hippocampal CA1 pyramidal neurons brief bursts high-frequency stimulation (HFS) θ burst (TBS) with long-lasting enhanced responses (long-term potentiation [LTP]), albeit by engaging signaling pathways. TBS induces LTP through calpain-1-mediated suprachiasmatic nucleus circadian oscillatory...
The ampakine CX614 positively modulates alpha-amino-3-hydroxy-5methyl-4-isoxazolepropionic acid (AMPA) receptor-gated currents and increases brain-derived neurotrophic factor (BDNF) expression. In rat hippocampal slice cultures, rapidly BDNF gene expression but with time, mRNA levels fall despite the continued presence of active drug. present study examined this apparent refractory period possibility that spaced treatments could sustain elevated protein levels. cultured slices, CX614, a...
Calpain is a calcium-dependent protease that plays significant role in synaptic plasticity, cell motility, and neurodegeneration. Two major calpain isoforms are present brain, with μ-calpain (calpain1) requiring micromolar calcium concentrations for activation m-calpain (calpain2) needing millimolar concentrations. Recent studies fibroblasts indicate epidermal growth factor (EGF) can activate independently of via mitogen-activated protein kinase (MAPK)-mediated phosphorylation. In neurons,...
17-Beta-estradiol (E2) is a steroid hormone involved in numerous brain functions. E2 regulates synaptic plasticity part by enhancing NMDA receptor function and spine density the hippocampus, resulting increased long-term potentiation facilitation of learning memory. As calcium-dependent neutral protease, calpain, also these processes, we tested whether could activate calpain examined functional consequences E2-mediated activation hippocampus. Calpain activity was analyzed fluorescence...
Memory consolidation has been suggested to be protein synthesis dependent. Previous data indicate that BDNF-induced dendritic is a key event in memory formation through activation of the mammalian target rapamycin (mTOR) pathway. BDNF also activates calpain, calcium-dependent cysteine protease, which shown play critical role learning and memory. This study was therefore directed at testing hypothesis calpain activity required for BDNF-stimulated local synthesis, identifying underlying...
Angelman syndrome (AS) is a neurogenetic disorder caused by deficiency of maternally expressed ubiquitin-protein ligase E3A (UBE3A), an E3 that targets specific proteins for proteasomal degradation. Although motor function impairment occurs in all patients with AS, very little research has been done to understand and treat it. The present study focuses on Ube3A deficiency-induced alterations signaling through the mechanistic target rapamycin (mTOR) pathway cerebellum AS mouse model potential...
Alzheimer's disease (AD) is characterized by progressive cognitive deficits, accumulation of amyloid-β (Aβ) and intracellular neurofibrillary tangles, neuronal death. Additionally, mitochondrial dysfunction free radical damage are hallmarks o
Dendritic protein synthesis and actin cytoskeleton reorganization are important events required for the consolidation of hippocampal LTP memory. However, temporal spatial relationships between these two processes remain unclear. Here, we report that treatment adult rat slices with BDNF or tetraethylammonium (TEA), which induces a chemical form LTP, produces rapid transient increase in RhoA levels. Changes were restricted to dendritic spines CA3 CA1 require de novo regulated by mammalian...
Estrogen is an important modulator of hippocampal synaptic plasticity and memory consolidation through its rapid action on membrane-associated receptors. Here, we found that both estradiol the G-protein–coupled estrogen receptor 1 (GPER1) specific agonist G1 rapidly induce brain-derived neurotrophic factor (BDNF) release, leading to transient stimulation activity-regulated cytoskeleton-associated (Arc) protein translation GluA1-containing AMPA internalization in field CA3 hippocampus. We...