A5060134414- Aquaculture disease management and microbiota
- Glioma Diagnosis and Treatment
- Estrogen and related hormone effects
- Neuroscience and Neuropharmacology Research
- Invertebrate Immune Response Mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Bladder and Urothelial Cancer Treatments
- Cancer, Hypoxia, and Metabolism
- Reproductive biology and impacts on aquatic species
- Epigenetics and DNA Methylation
- bioluminescence and chemiluminescence research
- Nicotinic Acetylcholine Receptors Study
- Computational Drug Discovery Methods
- RNA Interference and Gene Delivery
- Myxozoan Parasites in Aquatic Species
- Receptor Mechanisms and Signaling
- Nanoparticle-Based Drug Delivery
- Brain Metastases and Treatment
- Cancer Cells and Metastasis
- Protein Degradation and Inhibitors
- Urological Disorders and Treatments
- Pharmacological Effects of Natural Compounds
- RNA modifications and cancer
- Cancer Research and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
Massachusetts General Hospital
2014-2025
Shandong Normal University
2025
Ministry of Agriculture and Rural Affairs
2022-2024
Shandong Agricultural University
2018-2024
Liaocheng University
2024
Yantaishan Hospital
2015-2024
Ningbo University
2021-2022
Harvard University
2011-2020
Harvard NeuroDiscovery Center
2017
Brigham and Women's Hospital
2016
Research Article10 February 2016Open Access Source DataTransparent process Therapeutic potential of targeting microRNA-10b in established intracranial glioblastoma: first steps toward the clinic Nadiya M Teplyuk Department Neurology, Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA Search more papers by this author Erik J Uhlmann Galina Gabriely Natalia Volfovsky Simons Foundation, New York, NY, Yang Wang Jian Teng Massachusetts...
Personalized cancer treatments using combinations of drugs with a synergistic effect is attractive but proves to be highly challenging. Here we present an approach uncover the efficacy drug based on analysis mono-drug effects. For this used dose-response data from pharmacogenomic encyclopedias and represent these as atlas. The atlas represents relations between effects allows identify independent processes for which tumor might particularly vulnerable when attacked by two drugs. Our enables...
We reported previously that both subtypes of estrogen receptors, ERalpha and ERbeta, are expressed by human urothelial cells mediate estrogen-induced cell proliferation in these cells. The aim this study was to determine the extent which each ER subtype contributes their possible involvement regulation cycle. compared expression ERbeta mRNAs protein quantitatively primarily cultured bladder obtained from six individuals with three immortalized (E6, E7, UROtsa) two cancer lines (HTB-9 T24)....
Bioluminescence imaging (BLI) has shown to be crucial for monitoring in vivo biological processes. So far, only dual bioluminescence using firefly (Fluc) and Renilla or Gaussia (Gluc) luciferase been achieved due the lack of availability other efficiently expressed luciferases different substrates. Here, we characterized a codon-optimized from Vargula hilgendorfii (Vluc) as reporter mammalian gene expression. We showed that Vluc can multiplexed with Gluc Fluc sequential three distinct...
Highlights•SCD1 is essential for GSC maintenance and confers tumor growth advantage in vivo•Therapeutic targeting of SCD1 highly effective GBM mouse models•SCD1 exerts a cytoprotective function GSCs by regulating ER homeostasis•ER stress promotes lipogenesis through the activation IRE1-SREBP1-SCD1 axisSummaryInherent plasticity various survival cues allow glioblastoma stem-like cells (GSCs) to survive proliferate under intrinsic extrinsic conditions. Here, we report that depend on adaptive...
Molecular profile of glioblastoma multiforme (GBM) revealed 4 subtypes, 2 which, proneural and mesenchymal, have been predominantly observed, with the latter displaying a more aggressive phenotype increased therapeutic resistance. Single-cell RNA sequencing that multiple subtypes actually reside within same tumor, suggesting cellular heterogeneity in GBM. Further, plasticity between these is observed during tumor recurrence response to radiation therapy. Patient-derived GBM stemlike cells...
In recent years, large-scale afforestation in the Yellow River Basin (YRB) has attracted widespread attention due to its significant impact on surface water, playing a crucial role ecological sustainability and high-quality development of basin. this study, we used combination Theil–Sen Mann–Kendall trend analysis detect spatiotemporal dynamic changes NDVI, water storage (SWS), components YRB from 2001 2020, explored time lag instantaneous effects between them using methods such as...
The coupling of liquid chromatography (LC) to mass spectrometry (MS) is an accurate and proven technique for identifying different types RNA modifications. However, due the lack standards analytical biases, epitranscriptome-wide quantitative MS analysis modifications challenging. Here, we report development a standard-free (SqMS) method measuring multiple Starting from extraction total LC-MS digested RNA, commonly used approach in SqMS. To eliminate biases that resulted ionization...
We have previously shown that estrogen stimulates cell proliferation in both normal and transformed urothelial cells mainly through activation of the two primary receptors (ERs), ERα ERβ. A growing body evidence suggests also initiates nongenomic effects cannot be explained by ERs. In present study, we observed express high amounts GPR30, a G protein-coupled receptor recently identified as candidate for membrane-associated binding. Membrane- impermeable bovine serum albumin-conjugated...
Glioblastoma is the most common malignant primary brain tumor. Temozolomide (TMZ) standard chemotherapeutic agent for this disease. However, intrinsic and acquired TMZ-resistance represents a major obstacle therapy. In order to identify factors involved in TMZ-resistance, we engineered different TMZ-resistant glioblastoma cell lines. Gene expression analysis demonstrated that EFEMP1, an extracellular matrix protein, associated with phenotype. Silencing of EFEMP1 cells resulted decreased...
Northern snakehead (Channa argus) is an indigenous fish species and one of popularly cultured snakeheads in China other Asian countries. Unfortunately, Nocardia seriolae infections have caused considerable losses the aquaculture industry. However, infectivity immune response induced by N. are unclear. In order to better understand a series time points after challenge, we conducted transcriptomic comparison spleen at 48, 96, 144 h challenge seriola against their control counterparts. Gene...
Nowadays, the molecular mechanisms governing TSD (temperature-dependent sex determination) or GSD + TE (genotypic determination temperature effects) remain a mystery in fish.We developed three all-female families of Nile tilapia (Oreochromis niloticus), and family with highest male ratio after high-temperature treatment was used for transcriptome analysis.First, gonadal histology analysis indicated that histological morphology control females (CF) not significantly different from...
Abstract Background Glioblastoma (GBM) is the most common and aggressive primary malignant brain tumor. Standard-of-care treatment involves maximal surgical resection of tumor followed by radiation chemotherapy (temozolomide [TMZ]). The 5-year survival rate patients with GBM <10%, a colossal failure that has been partially attributed to intrinsic and/or acquired resistance TMZ through O6-methylguanine DNA methyltransferase (MGMT) promoter methylation status in Methods A drug screening...
The olfactory ensheathing cells (OECs) migrate from the peripheral nervous system to central (CNS), a critical process for development of and axonal extension after injury in neural regeneration. Because their ability site anti-inflammatory properties, OECs were tested against different neurological pathologies, but never studied context cancer. Here, we evaluated OEC tropism gliomas potential as "Trojan horse" deliver therapeutic transgenes through nasal pathway, natural route CNS.OECs...
The tumor-tropic properties of neural stem cells (NSCs) have been shown to serve as a novel strategy deliver therapeutic genes tumors. Recently, we reported that the cardiac glycoside lanatoside C (Lan C) sensitizes glioma anticancer agent tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Here, engineered an FDA-approved human NSC line synthesize and secrete TRAIL Gaussia luciferase (Gluc) blood reporter. We showed upon systemic injection, these selectively migrate toward...
Both nerve growth factor (NGF) and estrogen have been shown to stimulate proliferation of various cell types. Human urothelial cells (HUC) express the α- β-subtypes receptor (ER α ER β ) as well tyrosine kinase A (trkA), high-affinity for NGF. We investigated interactions between NGF relative using primary cultures HUC. 17β-estradiol (E2) stimulated synthesis by HUC, E2 (50 nM), agonist 16α-iodo-17β-estradiol (10 or genistein nM) each HUC proliferation, an effect that was abolished...
is a significant pathogen affecting shrimp and crab farming, particularly strains carrying genes associated with acute hepatopancreatic necrosis syndrome. However, the immune response of