A5055721229- Pancreatic function and diabetes
- Hearing, Cochlea, Tinnitus, Genetics
- Reproductive tract infections research
- Microbial infections and disease research
- Vestibular and auditory disorders
- Dendrimers and Hyperbranched Polymers
- Diabetes and associated disorders
- Endoplasmic Reticulum Stress and Disease
- RNA Interference and Gene Delivery
- Plant Virus Research Studies
- Signaling Pathways in Disease
- Electron Spin Resonance Studies
- Cardiac electrophysiology and arrhythmias
- Advanced Neuroimaging Techniques and Applications
- Immunotherapy and Immune Responses
- Erythrocyte Function and Pathophysiology
- Antimicrobial Peptides and Activities
- Hemoglobin structure and function
- Pain Mechanisms and Treatments
- Connexins and lens biology
- Nerve injury and regeneration
- Autophagy in Disease and Therapy
- Genetics and Neurodevelopmental Disorders
- Dietary Effects on Health
- Hearing Loss and Rehabilitation
John D. Dingell VA Medical Center
2021-2025
Wayne State University
2011-2025
Eugene Applebaum College of Pharmacy and Health Sciences
2021-2022
Abstract The present study was designed to explore whether the depletion of serotonin (5‐HT) in central nervous system (CNS 5‐HT ) leads systolic and diastolic dysfunction this is exacerbated by sex, age spinal cord injury. Echocardiographic assessment function completed young old male female tryptophan hydroxylase 2 knockout (TPH2 −/− wild‐type +/+ mice with intact cords, as well C hemisected TPH2 mice. In addition, lumbar sympathetic activity recorded elderly Systolic evident mice,...
Effective personalized therapeutic treatment for hearing loss is currently not available. Cochlear oxidative stress commonly identified in the pathogenesis of based upon findings from excised tissue, thus suggesting a promising druggable etiology. However, timing and site(s) to target anti-oxidant vivo are clear. Here, we address this long-standing problem with QUEnch-assiSTed Magnetic Resonance Imaging (QUEST MRI), which non-invasively measures excessive production free radicals without an...
Published evidence implicates Caspase recruitment domain containing protein 9 (CARD9) in innate immunity. Given its recently suggested roles obesity and insulin resistance, we investigated regulatory role(s) the onset of islet beta cell dysfunction under chronic hyperglycemic (metabolic stress) conditions.Islets from mouse pancreas were isolated by collagenase digestion method. Expression CARD9 was suppressed INS-1 832/13 cells siRNA transfection using DharmaFect1 reagent. The degree...
Published evidence suggests regulatory roles for small G proteins (Cdc42 and Rac1) in glucose-stimulated insulin secretion (GSIS) from pancreatic beta-cells. More recent novel these proteins, specifically Rac1, the induction of metabolic dysfunction islet beta-cell under duress a variety stress conditions. However, potential upstream regulators sustained activation Rac1 have not been identified beta-cell. Recent studies other cell types RhoG, protein, as an regulator specific experimental...
Abstract Reliable methods for repetitive and longitudinal assessment of central vestibular pathway function in vivo are rather limited. Manganese-enhanced magnetic resonance imaging (MEMRI) has been used various sensory systems to evaluate neuronal activity pathways, but MEMRI pathways minimal. The present study addressed this gap knowledge by assessing whether Mn 2+ can be taken up an activity-dependent manner through voltage-gated calcium channels the nuclear complex (VNC)...
Background/Aims: We recently reported increased phosphorylation (at S536) of the p65 subunit NFκB (Rel A) in pancreatic beta (INS-1 832/13) cells following exposure to hyperglycemic (HG) conditions. also demonstrated that HG-induced S536 is downstream regulatory effects CARD9 since deletion expression significantly attenuated cells. The overall objective current investigation identify putative mechanisms underlying islet HG Methods: INS-1 832/13 were incubated low glucose (LG; 2.5 mM) or...
CARD9, a scaffolding protein, has been implicated in the pathogenesis of metabolic diseases, including obesity and diabetes. We recently reported novel roles for CARD9 islet β-cell dysregulation under duress gluco (HG)- glucolipotoxic (GLT) stress. expression was also increased β-cells following exposure to HG GLT The current study is aimed at understanding putative histone deacetylation HG- GLT-induced CARD9. Using two structurally distinct inhibitors deacetylases (HDACs), namely...
Published evidence implicates Caspase recruitment domain containing protein 9 (CARD9) in innate immunity. We recently reported expression of CARD9 human islets, rodent islets and clonal beta cells, demonstrated its role glucose-stimulated insulin secretion (GSIS). Given suggested roles obesity resistance, we investigated regulatory role(s) the onset islet cell dysfunction under chronic hyperglycemic (metabolic stress) conditions. is significantly increased following exposure to high glucose,...