A5059835752- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Trypanosoma species research and implications
- RNA Interference and Gene Delivery
- Research on Leishmaniasis Studies
- Parasites and Host Interactions
- Extracellular vesicles in disease
- Biosensors and Analytical Detection
- Analytical Chemistry and Sensors
- Parasitic Infections and Diagnostics
- Viral Infections and Immunology Research
- Lipid Membrane Structure and Behavior
- Drug-Induced Hepatotoxicity and Protection
- Cancer-related molecular mechanisms research
- 3D Printing in Biomedical Research
- COVID-19 diagnosis using AI
- SARS-CoV-2 detection and testing
- IoT-based Smart Home Systems
- Biochemical and Molecular Research
- Molecular Sensors and Ion Detection
- Cell Image Analysis Techniques
- Cytomegalovirus and herpesvirus research
- MXene and MAX Phase Materials
- Spectroscopy Techniques in Biomedical and Chemical Research
- Molecular Biology Techniques and Applications
Parque Tecnológico de la Salud
2016-2024
Universidad de Granada
2020
The COVID-19 pandemic has highlighted the need for innovative approaches to its diagnosis. Here we present CoVradar, a novel and simple colorimetric method that combines nucleic acid analysis with dynamic chemical labeling (DCL) technology Spin-Tube device detect SARS-CoV-2 RNA in saliva samples. assay includes fragmentation step increase number of templates analysis, using abasic peptide probes (DGL probes) immobilized nylon membranes specific dot pattern capture fragments. Duplexes are...
Circulating microRNAs have been identified as potential biomarkers for early detection, prognosis, and prediction of several diseases. Their use in clinical diagnostics has limited by the lack suitable detection techniques. Most current technologies suffer from requiring complex protocols, not yet able to deliver robust cost-effective assays field diagnostics. In this work, we report development a breakthrough platform profiling circulating microRNAs. The comprises novel silicon...
MicroRNAs (miRs) have emerged as promising biomarkers for early disease diagnosis and personalized treatment monitoring. However, their clinical utility has been hampered by technical limitations. Dynamic chemical labeling (DCL) based on capturing abasic PNA probes reactive nucleobases, known SMART bases, is a PCR-free approach that proven very useful the direct interrogation of circulating miRs. In this work, we expand palette tools available detection DCL miR synthesizing new nucleobase...
A novel and unique method for a PCR/label-free detection absolute quantification of miR-451a in blood samples.
miRNA analysis simplified using multi-ChemiRNA Tech.
Abstract Leishmaniasis and Chagas disease are endemic in many countries, re-emerging the developed countries. A rapid accurate diagnosis is important for early treatment reducing duration of infection as well preventing further potential health complications. In this work, we have a novel colorimetric molecular assay that integrates nucleic acid analysis by dynamic chemistry (ChemNAT) with reverse dot-blot hybridization an array format easy discrimination Leishmania major Trypanosoma cruzi ....
A smartphone-based platform for the diagnosis of parasitic infections has been developed, tested and validated. The system is capable making automatic accurate analysis millimetric colorimetric arrays in centrifuge collection tubes, which are well established tools used clinical analysis. To that end, an Android-based software application use smartphone rear camera, enabling precise image processing spot arrays. low-cost plastic accessory developed using 3D-printing to provide controlled...
MicroRNAs (miRs) have emerged as promising biomarkers for early disease diagnosis and personalized treatment monitoring. However, their clinical utility has been hampered by technical limitations. Dynamic chemical labeling (DCL) based on capturing abasic PNA probes reactive nucleobases, known SMART bases, is a PCR-free approach that proven very useful the direct interrogation of circulating miRs. In this work, we expand palette tools available detection DCL miR synthesizing new nucleobase...
Leishmania (L.) infantum causes visceral, cutaneous, and mucosal leishmaniasis in humans canine dogs. Herein, we describe that O-alkyl hydroxamate derivatives displayed potent selective vitro activity against the amastigote stage of L. while no was observed promastigotes. Compound 5 showed vivo infantum. Moreover, combination compound supported on gold nanoparticles meglumine antimoniate also effective improved these compounds compared to individual treatment. Docking studies did not reach...
Drug-induced liver injury (DILI) is a potentially fatal adverse event and leading cause for pre- post-marketing drug withdrawal. Several multinational DILI initiatives have now recommended panel of protein microRNA (miRNA) biomarkers that can detect early inform about mechanistic basis. This manuscript describes the development seqCOMBO, unique combo-multiplexed assay which combines dynamic chemical labelling approach an antibody-dependant method on Luminex MAGPIX system. SeqCOMBO enables...
MicroRNAs (miRNAs) are considered potential biomarkers due to their specific expression patterns in tissues and changes under pathological conditions. MicroRNA-122 (miR-122) is a vertebrate-specific miRNA known for its high the liver, where it plays role hepatic metabolism, liver development cellular differentiation. Dysregulation of miR-122 has been found conditions such as hepatocellular carcinoma or drug-induced injury (DILI). Currently, detection faces challenges improving point-of-care...
MicroRNAs (miRNAs) have been recognised as potential biomarkers due to their specific expression patterns in different biological tissues and changes under pathological conditions. MicroRNA-122 (miR-122) is a vertebrate-specific miRNA that predominantly expressed the liver plays an important role metabolism development. Dysregulation of miR-122 associated with several liver-related diseases, including hepatocellular carcinoma drug-induced injury (DILI). Given biomarker, its effective...
COVID-19 pandemic has pointed out the strong need to continue promoting development of innovative approaches for rapid, safe handling and accurate molecular diagnosis SARS-CoV-2 infection. In this work, we have developed CoVradar, a novel colourimetric detection system that integrates nucleic acid analysis by dynamic chemistry labelling (DCL) technology with Spin-Tube devices allowing reverse dot-blot hybridization in an array format detect RNA within saliva specimens. The assay is designed...