A5074148162- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Ion channel regulation and function
- Systemic Lupus Erythematosus Research
- Inflammatory Bowel Disease
- Pharmacological Effects and Assays
- Peptidase Inhibition and Analysis
- Viral Infections and Immunology Research
- Ion Transport and Channel Regulation
- Regulation of Appetite and Obesity
- Microscopic Colitis
- Cardiac electrophysiology and arrhythmias
- Neurotransmitter Receptor Influence on Behavior
- Apelin-related biomedical research
- thermodynamics and calorimetric analyses
- Proteoglycans and glycosaminoglycans research
- Viral Infectious Diseases and Gene Expression in Insects
- Biosimilars and Bioanalytical Methods
- Lipid metabolism and disorders
- Cell Adhesion Molecules Research
- Cynara cardunculus studies
- Cholesterol and Lipid Metabolism
- Nicotinic Acetylcholine Receptors Study
- Heat shock proteins research
- Redox biology and oxidative stress
Institut Clinique de la Souris
2024
Institut de Biologie Moléculaire et Cellulaire
2003-2015
Centre National de la Recherche Scientifique
2000-2015
Institut de Science et d'Ingénierie Supramoléculaires
2014
Bellvitge University Hospital
2014
Immunologie, Immunopathologie et Chimie Thérapeutique
2000-2012
Hôpital Fernand-Widal
2011
Forschungsinstitut Technologie und Behinderung
2009
Institut de Biologie Moléculaire des Plantes
2005
Institut de Virologie
2002
The 52-kDa SSA/Ro (Ro52) ribonucleoprotein is an antigenic target strongly associated with the autoimmune response in mothers whose children develop neonatal lupus and congenital heart block. When sera from patients systemic erythematosus were used as controls enzyme immunoassay to screen for antibodies against human serotoninergic 5-HT4-receptor, a high correlation was found between presence of anti-Ro52 protein such reacting synthetic peptide, corresponding second extracellular loop 5-HT4...
A deficient activity of one or more the mitochondrial oxidative phosphorylation (OXPHOS) enzyme complexes leads to devastating diseases, with high unmet medical needs. Mitochondria, and specifically OXPHOS system, are main cellular production sites Reactive Oxygen Species (ROS). Increased ROS production, ultimately leading irreversible damage macromolecules selective reversible redox modulation cell signalling, is a causative hallmark diseases. Here we report on development new...
RT–PCR technique was used to clone the human 5‐HT 4(e) receptor (h5‐HT ) from heart atrium. We showed that this h5‐HT splice variant is restricted brain and Recombinant stably expressed in CHO C6‐glial cell lines at 347 88 fmol mg −1 protein, respectively. Expression of receptors membrane confirmed by immunoblotting. The binding profile, determined competition with [ 3 H]‐GR113808 a number 4 ligands, consistent previously reported for other isoforms. Surprisingly, we found rank order...
Abstract Evodiamine, a bioactive alkaloid from the fruits of traditional Chinese medicine Evodia rutaecarpa (Juss.) Benth. (Fructus Evodiae, Wuzhuyu), recently gained attention as dietary supplement for weight loss and optimization lipid metabolism. In light its use by patients consumers, there is an urgent need to elucidate molecular targets affected this natural product. Using novel interactomics approach, Nematic Protein Organisation Technique (NPOT), we report identification ATP-binding...
We have previously suggested that the recognition of a cross-reactive epitope on 5-HT4 receptor and 52-kDa SSA / Ro protein by serotonin-antagonizing autoantibodies could explain electrophysiological symptoms congenital heart block in neonatal lupus. To confirm this hypothesis, we immunized female mice with four synthetic peptides corresponding to recognized epitopes. All developed anti-peptide antibodies, which cross-reacted Ro52 both cognate proteins. Peptide-immune were mated. The pups...
Among the five human 5‐HT 4 (h5‐HT ) receptor isoforms, h5‐HT 4(a) was studied with a particular emphasis on molecular interactions involved in ligand binding. For this purpose, we used site‐directed mutagenesis of transmembrane domain. Twelve mutants were constructed special focus residue P4.53 helix IV which substitutes receptors highly conserved S among rhodopsin family receptors. The mutated transiently expressed COS‐7 cells. Ligand binding or competition studies two agonists, serotonin...
GnRH vaccines have been successfully used for the inhibition of gonadotropin secretion and gonadal function. As an alternative to native GnRH, retro-inverso (RI) might be improved immunogen. The RI peptides are composed D-amino acids assembled in reverse order (C N terminus) relation parent L peptide. These immunogenic can produce high titers antibodies that bind peptide with affinity specificity. We show RI-GnRH conjugated ovalbumin as well unconjugated elicit anti-GnRH rabbits mice....
Monoclonal antibodies were raised against a recombinant ribosomal P2β protein of Trypanosoma cruzi. One these reacted with the C terminus this (peptide R13, EEEDDDMGFGLFD) and epitope mapping confirmed that was same as one defined by serum immunized mice, similar to previously described chronic Chagas' heart disease (cChHD) anti-P epitope. Western blotting showed monoclonal antibody recognized parasite P proteins, well human proteins. Electron microscopy it stained different structures in...
Antibodies directed against the second extracellular loop of G protein-coupled receptors were shown to possess functional activities. Using a monoclonal antibody human β2-adrenergic receptor, scFv fragment with high affinity for target epitope was constructed and produced. The recognized on A431 cells, blocked cAMP accumulation induced by β2-agonist salbutamol, decreased basal in same cells. Their vitro activity tested neonatal rat cardiomyocytes. fragments chronotropic clenbuterol. They...
Abstract The knowledge about the features of energy metabolism in MAFLD population living at different climatic and geographic heights is lacking. goal this study to explore biochemical parameters blood erythrocyte consumption patients with without DM2 low- moderate-altitude regions Central Asia. Our was carried out on low-altitude mountains: Bishkek, altitude=750–800 m; n=67 (MAFLD DM 2: n=24; DM2: n=25; control: n=18), At-Bashy District, Naryn Region, altitude=2046–2300 n=58 n=28; n=18;...
Autoantibodies in vitro modulating the M2 acetylcholine receptor (M2ACh-R) were observed patients with idiopathic dilated cardiomyopathy (IDC) or Chagas' (ChC). We investigated vivo consequences on heart rate of such antibodies mice immunized a peptide derived from second extracellular loop M2ACh-R compared an irrelevant peptide. Sera M2ACh-R-derived recognized immunoblots and enhanced agonist activity carbachol toward M2AChR transfected CHO cells. In vivo, no difference could be shown...
The melanocortin-4 receptor (MC4R) is part of an important pathway regulating energy balance. Here we report the existence autoantibodies (autoAbs) against MC4R in sera obese patients.The autoAbs were detected after screening 216 patients' by using direct and inhibition ELISA with N-terminal sequence MC4R. Binding to native was evaluated flow cytometry, pharmacological effects measuring adenylyl cyclase activity.Positive results all tests obtained patients overweight or obesity (prevalence,...
The presence of functional 5-HT4 receptors in human and its involvement neonatal lupus erythematosus (NLE) have prompted us to study the receptor expression role during embryogenesis. Earlier we managed demonstrate that female BALB/c mice immunized against second extracellular loop (SEL) gave birth pups with heart block. To explain this phenomenon investigated mouse At same time looked whether consequence immunomodulation observed earlier is relation expression. We studied at mRNA level two...
The purpose of this study was to investigate the frequency occurrence autoantibodies against G-protein coupled cardiovascular receptors and their relation clinical manifestation hypertrophic cardiomyopathy (HCM). Autoantibodies beta1-receptors, Muscarin-2-receptors, Angiotensin-II-receptor subtype 1 alpha1-receptors were determined with ELISA in 52 patients HCM (37 male, 15 female, mean age 55 +/- years) 40 healthy, sex matched controls. characterization HCM-patients included ECG, 24-h...
We applied for the first time 2 label-free technologies, physiological intermolecular modulation spectroscopy (PIMS) and nematic protein organization technic (NPOT) in anti-tumor necrosis factor (TNF) refractory inflammatory bowel disease (IBD) patients to identify clinical responders vedolizumab therapy elucidate their underlying functional molecular network.PIMS analysis was performed peripheral blood taken prior application 20 IBD (Crohn n = 13; ulcerative colitis 7) at least 1 previous...
The links between infections and the development of B-cell-mediated autoimmune diseases are still unclear. In particular, it has been suggested that infection-induced stimulation innate immune sensors can engage low affinity autoreactive B lymphocytes to mature produce mutated IgG pathogenic autoantibodies. To test this hypothesis, we established a new knock-in mouse model in which cells could be committed an maturation process. We show chronic bacterial infection allows activation such...
<b><i>Background/Aims:</i></b> Anti-tumor necrosis factor (TNF) antibodies have clinical efficiency only in a subgroup of patients with inflammatory bowel diseases (IBD). Prediction response is critical problem. Physiological intermolecular modification spectroscopy (PIMS) label-free technology performed physiological conditions. PIMS enables real-time monitoring dynamic molecular resonance entire proteins and macromolecules an individual. The aim this study was to...
We have previously reported that antipeptide antibodies directed against the second extracellular loop of cardiac h5-HT4 receptor could block activation L-type Ca channel in human atrial cardiomyocytes. In this paper we investigate immunological and physiological activity these antibodies, a cell system expressing larger amount receptors than cells. The recombinant was expressed at surface COS-7 cells under an active form (serotonin, EC50 = 1.81 x 10(-7) M), high level (375 +/- 25 fmol...