The modern age of metagenomics has delivered unprecedented volumes data describing the genetic and metabolic diversity bacterial communities, but it failed to provide information about coincident cellular morphologies. Much like biosynthetic capabilities, morphology comprises a critical component fitness, molded by natural selection into many elaborate shapes observed across domain. In this essay, we discuss its implications for understanding both mechanistic adaptive basis morphogenesis. We...

A number of bacterial metal transporters belong to the ABC transporter family. To better understand structural determinants selectivity one such transporter, we previously determined structure periplasmic domain a zinc ZnuA, from Synechocystis 6803 and found that ZnuA binds via three histidines. Unique these transporters, has highly charged mobile loop protrudes protein in vicinity binding site had suggested might facilitate acquisition. further examine function this loop, properties two...

Cyanobacteria account for a significant percentage of aquatic primary productivity even in areas where the concentrations essential micronutrients are extremely low. To better understand mechanism iron selectivity and transport, structure solute binding domain an ATP cassette transporter, FutA1, was determined presence absence iron. The ion is bound within "C-clamp" via four tyrosine one histidine residues. There extensive interactions between these ligating residues rest protein such that...

Agrobacterium tumefaciens , a significant biotechnological tool for production of transgenic plant lines, is highly resistant to wide variety antibiotics, posing challenges various applications. One challenge the efficient elimination A. from transformed tissue without using levels antibiotics that are toxic plants.

LytM-domain containing proteins are LAS peptidases (lysostaphin-type enzymes, D-Ala-D-Ala metallopeptidases, and sonic hedgehog) known to play diverse roles throughout the bacterial cell cycle through direct or indirect hydrolysis of wall. A subset LytM factors catalytically inactive but regulate activity other wall hydrolases classically described as separation NlpD EnvC. Here, we explore function four in alphaproteobacterial plant pathogen Agrobacterium tumefaciens . An LmdC ortholog...

In contrast to the majority of voltage-gated ion channels, hyperpolarization-activated channels remain closed at depolarizing potentials and are activated hyperpolarizing potentials. The basis for this reverse polarity is thought be a result differences in way voltage-sensing domain (VSD) couples pore domain. absence structural data, molecular mechanism coupling remains poorly characterized. Here we report characterization structure local dynamics activation gate (lower S6 region) MVP,...

ABSTRACT Fretibacter rubidus is a dimorphic, prosthecate member of the family Maricaulaceae , order Caulobacterales, in class Alphaproteobacteria. Here, we report genome type strain JCM 15585 T (JC2236 ), sequenced with Oxford Nanopore Technology.

ABSTRACT Glycocaulis abyssi is a dimorphic, prosthecate member of the family Maricaulaceae , order Caulobacterales, in class Alphaproteobacteria, originally isolated from an abyssal hydrothermal vent. Here, we report complete genome sequence type strain LMG 27140 T (MCS33 ) obtained using Oxford Nanopore technology.

ABSTRACT Algimonas porphyrae is a dimorphic, prosthecate member of the family Robigintomaculaceae , order Caulobacterales, in class Alphaproteobacteria, originally isolated from red alga Porphyra yezoensis . Here we report complete genome type strain LMG 26424 T (0 C-2-2 ) obtained by sequencing with Oxford Nanopore technology.

ABSTRACT The remarkable ability of Agrobacterium tumefaciens to transfer DNA plant cells has allowed the generation important transgenic crops. One challenge A. -mediated transformation is eliminating bacteria after prevent detrimental effects plants and release engineered environment. Here we use a reverse genetics approach identify genes involved in ampicillin resistance with goal utilizing these antibiotic-sensitive strains for transformations. We show that treating C58 led increased...

Summary Temperate phages constitute a potentially beneficial genetic reservoir for bacterial innovation despite being selfish entities encoding an infection cycle inherently at odds with fitness. These integrate their genomes into the host during infection, donating new, but deleterious, material: phage genome encodes toxic genes, such as lysins, that kill bacterium cycle. Remarkably, some bacteria have exploited destructive properties of genes own benefit by co-opting them toxins functions...

Temperate phages constitute a potentially beneficial genetic reservoir for bacterial innovation despite being selfish entities encoding an infection cycle inherently at odds with fitness. These integrate their genomes into the host during infection, donating new, but deleterious, material: phage genome encodes toxic genes, such as lysins, that kill bacterium cycle. Remarkably, some bacteria have exploited destructive properties of genes own benefit by co-opting them toxins functions related...