A5079033540- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Neurobiology and Insect Physiology Research
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Neuroinflammation and Neurodegeneration Mechanisms
- Physiological and biochemical adaptations
- Cellular transport and secretion
- Neural dynamics and brain function
- Zebrafish Biomedical Research Applications
- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- Traditional Chinese Medicine Analysis
- Single-cell and spatial transcriptomics
- NF-κB Signaling Pathways
- Genetics and Neurodevelopmental Disorders
- Wnt/β-catenin signaling in development and cancer
- Axon Guidance and Neuronal Signaling
- Ubiquitin and proteasome pathways
- Neuroscience and Neural Engineering
- Cell death mechanisms and regulation
- Sulfur Compounds in Biology
Hangzhou Normal University
2015-2024
Zhejiang University
2022
First Affiliated Hospital Zhejiang University
2022
Wenzhou University
2022
Scripps Research Institute
2011-2018
Jiangsu University of Science and Technology
2011
Chinese Academy of Sciences
2003-2010
Shanghai Institute of Applied Physics
2010
Shanghai Institutes for Biological Sciences
2003-2009
Cold Spring Harbor Laboratory
2009
Repetitive correlated activation of pre- and postsynaptic neurons induced long-term potentiation (LTP) synaptic transmission among hippocampal grown on a layer astrocytes (mixed cultures) but not cultured in glial conditioned medium. Supplement d -serine, an agonist for the glycine-binding site N -methyl- -aspartate (NMDA) receptors, enhanced NMDA receptor enabled LTP induction medium cultures. The both mixed cultures slices was suppressed by antagonists, glycine-binding-site blockers or...
Interactions between neurons and glial cells in the brain may serve important functions development, maintenance, plasticity of neural circuits. Fast neuron-glia synaptic transmission has been found hippocampal NG2 cells, a distinct population macroglia-like widely distributed brain. We report that these synapses undergo activity-dependent modifications analogous to long-term potentiation (LTP) at excitatory synapses, hallmark neuronal plasticity. However, unlike induction LTP many...
Endocytosis of Trk (tropomyosin-related kinase) receptors is critical for neurotrophin signal transduction and biological functions. However, the mechanism governing endocytosis TrkB kinase B) specific contributions to downstream signaling are unknown. In this study, we report that blocking clathrin, dynamin, or AP2 in cultured neurons central nervous system inhibited brain-derived neurotrophic factor (BDNF)-induced activation Akt but not ERK. Treating with clathrin inhibitor...
NG2 cells originate from various brain regions and migrate to their destinations during early development. These express voltage-gated Na+ channels but fail produce typical action potentials. The physiological role of in these is unclear. We found that GABA induces membrane depolarization Ca2+ elevation cells, a process requiring activation GABAA receptors, channels, Na+/Ca2+ exchangers (NCXs), not channels. have identified persistent current may underlie the GABA-induced pathway prolonged...
Abstract The rapid development of high-throughput single-cell RNA sequencing technology offers a good opportunity to dissect cell heterogeneity animals. A large number organism-wide atlases have been constructed for vertebrates such as Homo sapiens , Macaca fascicularis Mus musculus and Danio rerio . However, an intermediate taxon that links mammals more ancient origin is still lacking. Here, we construct the first Xenopus landscape date, including larval adult organs. Common...
The balance of inhibitory to excitatory (I/E) synaptic inputs is thought control information processing and behavioral output the central nervous system. We sought test effects decreased or increased I/E ratio on visual circuit function visually guided behavior in Xenopus tadpoles. selectively transmission optic tectal neurons by knocking down γ2 subunit GABA(A) receptors (GABA(A)R) using antisense morpholino oligonucleotides expressing a peptide corresponding an intracellular loop subunit,...
Neural plasticity requires protein synthesis, but the identity of newly synthesized proteins generated in response to plasticity-inducing stimuli remains unclear. We used vivo bio-orthogonal noncanonical amino acid tagging (BONCAT) with methionine analog azidohomoalanine (AHA) combined multidimensional identification technique (MudPIT) identify that are tadpole brain over 24 hr. induced conditioning-dependent visual avoidance behavior, which required N-methyl-D-aspartate (NMDA) and...
Functional circuit assembly is thought to require coordinated development of excitation and inhibition, but whether they are co-regulated cell-autonomously remains unclear. We investigate effects decreased glutamatergic synaptic input on inhibitory synapses by expressing AMPAR subunit, GluA1 GluA2, C-terminal peptides (GluA1CTP GluA2CTP) in developing Xenopus tectal neurons. GluACTPs decrease excitatory inputs decreases Visually evoked currents proportionately, maintaining...
The emergence of dendritic arbor structure in vivo depends on synaptic inputs. We tested whether inhibitory GABAergic transmission regulates Xenopus optic tectal cell development by expressing a peptide corresponding to an intracellular loop (ICL) the gamma2 subunit type A GABA receptors (GABA(A)R), which is required anchor GABA(A) postsynaptic scaffold. Enhanced green fluorescent protein (EGFP)-tagged ICL (EGFP-ICL) was distributed punctate pattern at putative synapses, identified vesicular...
Abstract Nonmammalian vertebrates have a remarkable capacity to regenerate brain tissue in response central nervous system (CNS) injury. Nevertheless, it is not clear whether animals recover lost function after injury or injury‐induced cell proliferation mediates recovery. We address these questions using the visual and visually‐guided behavior Xenopus laevis tadpoles. established reproducible means produce unilateral focal optic tectal neurons without damaging retinotectal axons. then...
Experience-dependent synaptic plasticity refines brain circuits during development. To identify novel protein synthesis-dependent mechanisms contributing to experience-dependent plasticity, we conducted a quantitative proteomic screen of the nascent proteome in response visual experience Xenopus optic tectum using bio-orthogonal metabolic labeling (BONCAT). We identified 83 differentially synthesized candidate proteins (CPPs). The CPPs form strongly interconnected networks and are annotated...
In the developing central nervous system (CNS), progenitor cells differentiate into progeny to form functional neural circuits. Radial glial (RGs) are a transient cell type that is present during neurogenesis. It thought combination of trophic factors, neurotransmitters and electrical activity regulates proliferation differentiation RGs. However, it less clear how epigenetic modulation changes RG proliferation. We sought explore effect histone deacetylase (HDAC) on RGs in visual optic tectum...
Histone deacetylases (HDACs) are thought to localize in the nucleus regulate gene transcription and play pivotal roles neurogenesis, apoptosis plasticity. However, subcellular distribution of class I HDACs developing brain remains unclear. Here, we show that HDAC1 HDAC2 located both mitochondria Xenopus laevis stage 34 tectum mainly restricted following further development. HDAC3 is widely present mitochondria, cytoplasm during early tectal development distributed 45 tectum. In contrast,...
The in situ generation of H
Radial glial cells (RGs) are one of the important progenitor that can differentiate into neurons or glia to form functional neural circuits in developing central nervous system (CNS). Histone deacetylases (HDACs) has been associated with visual activity dependent changes BrdU-positive brain. We previously have shown HDAC1 is involved experience-dependent proliferation RGs. However, it less clear whether two other members class I HDACs, HDAC2 and HDAC3, regulation radial proliferation. Here,...