A5101466776- Gut microbiota and health
- Sperm and Testicular Function
- Nuts composition and effects
- Reproductive Biology and Fertility
- Phytoestrogen effects and research
- Cancer-related molecular mechanisms research
- Urinary Bladder and Prostate Research
- Ginseng Biological Effects and Applications
- Reproductive System and Pregnancy
- Effects and risks of endocrine disrupting chemicals
- Mycotoxins in Agriculture and Food
- Sexual function and dysfunction studies
- Burn Injury Management and Outcomes
- Plant Reproductive Biology
- Coccidia and coccidiosis research
- Plant Toxicity and Pharmacological Properties
- Agriculture, Soil, Plant Science
- Plant biochemistry and biosynthesis
- Carcinogens and Genotoxicity Assessment
- Zebrafish Biomedical Research Applications
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Chromosomal and Genetic Variations
- Single-cell and spatial transcriptomics
- Physiological and biochemical adaptations
Shenzhen University
2021-2025
Peking University Shenzhen Hospital
2018-2024
Zhejiang University
2022
First Affiliated Hospital Zhejiang University
2022
Army Medical University
2012-2017
Daping Hospital
2012-2017
Rationale: Busulfan is currently an indispensable anti-cancer drug, particularly for children, but the side effects on male reproduction are so serious that critical drug management needed to minimize any negative impact. Meanwhile, alginate oligosaccharides (AOS) natural products with many consequent advantages, have attracted a great deal of pharmaceutical attention. In current investigation, we performed single-cell RNA sequencing murine testes treated busulfan and/or AOS define...
Abstract Background Clinical data suggest that male reproductive dysfunction especially infertility is a critical issue for type 1 diabetic patient (T1D) because most of them are at the age. Gut dysbiosis involved in T1D related infertility. However, improved gut microbiota can be used to boost spermatogenesis and fertility remains incompletely understood. Methods was established ICR (CD1) mice with streptozotocin. Alginate oligosaccharide (AOS) (fecal transplantation (FMT) from AOS...
High-fat diet (HFD)-induced obesity is known to be associated with reduced male fertility and decreased semen quality in humans. HFD-related infertility a growing issue worldwide, it crucial overcome this problem ameliorate the distress of infertile couples. For first time, we discovered that fecal microbiota transplantation (FMT) alginate oligosaccharide (AOS)-improved gut (A10-FMT) ameliorated HFD-decreased (sperm concentration: 286.1 ± 14.1 versus 217.9 17.4 million/mL; sperm motility:...
Abstract The rapid development of high-throughput single-cell RNA sequencing technology offers a good opportunity to dissect cell heterogeneity animals. A large number organism-wide atlases have been constructed for vertebrates such as Homo sapiens , Macaca fascicularis Mus musculus and Danio rerio . However, an intermediate taxon that links mammals more ancient origin is still lacking. Here, we construct the first Xenopus landscape date, including larval adult organs. Common...
The male reproductive system has been the subject of considerable attention in recent years due to adverse effects Di (2-ethylhexyl) phthalate (DEHP). Although previous research suggested that DEHP exposure hinders early meiotic progression germ cells, underlying mechanisms are still not well understood. transcriptomic changes testicular cells postnatal rodents following were meticulously analyzed using 10X Genomics single-cell RNA sequencing this study. For downstream analysis, we acquired...
Bisphenol A (BPA), a chemical used in many consumer products, interferes with the endocrine system of mammals, including humans. The aim present study was to investigate effect BPA on spermatogenesis and semen quality. objective this assess effects mouse spermatogenesis. CD1 mice were all experiments. Mice treated different doses (0, 20 40 μg kg⁻¹ day⁻¹ from postnatal Day (PND) 3 PND21, PND 35 or PND49. After 5 weeks treatment, oestrogen receptor α expression increased testis, whereas...
Zearalenone (ZEA), a F-2 mycotoxin produced by Fusarium, has been found to be an endocrine disruptor through oestrogen receptor signalling pathway impair spermatogenesis. The disruption on reproductive systems ZEA exposure might transgenerational. In our previous report, we have that low dose (lower than no-observed effect level, NOEL) of impaired mouse spermatogenesis and decreased semen quality. purpose the current investigation was explore impacts low-dose in offspring after prenatal...
With the evolution of pandemic caused by Coronavirus disease 2019 (COVID-19), reverse transcriptase-polymerase chain reactions (RT-PCR) have invariably been a golden standard in clinical diagnosis. Nevertheless, traditional polymerase reaction (PCR) is not feasible for field application due to its drawbacks, such as time-consuming and laboratory-based dependence. To overcome these challenges, microchip-based ultrafast PCR system called SWM-02 was proposed make assay rapid, portable, low-cost...
Male factors-caused decline in total fertility has raised significant concern worldwide. LncRNAs have been identified to play various roles biological systems, including spermatogenesis. This study aimed explore the role of lncRNA5251 mouse spermatogenesis.The expression was modulated testes vivo or spermatogonial stem cells (C18-4 cells) vitro by shRNA.The sperm motility two generations mice after modulation (muF0 and muF1) decreased significantly overexpression lncRNA5251. GO enrichment...
Recently there has been a continuing worldwide decrease in the quality of human spermatozoa, especially spermatozoa motility and concentration.Many factors are involved this decline, great efforts have made to rescue spermatogenesis; however, little progress improvement sperm quality.Chestnuts used traditional Chinese medicine; their major active components chestnut polysaccharides (CPs).CPs many biological activities but effects on spermatogenesis unknown.The current investigation was...
Our previous study confirmed the beneficial effects of chestnut polysaccharides (CPs) on spermatogenesis process, but exact mechanism is not clear. Several studies have demonstrated importance balanced gut microbiota in maintaining normal reproductive function. In this study, we investigated biological functions CPs from perspective function, expecting to find out specific restoring impaired spermatogenesis. Compared with control group, mice treated busulfan showed a reduced number germ...
Introduction In recent years, the quality of male semen has been decreasing, and number infertilities caused by asthenozoospermia is increasing year year, diagnosis treatment patients with are gradually receiving attention whole society. Due to unknown etiology complex pathogenesis, there no specific for asthenozoospermia. Our previous study found that administration chestnut polysaccharide could alter intestinal microbiota thus improve testicular microenvironment, rescue impaired...
Abstract An increasing number of men with healthy and normal penises want to enlarge their penis. Therefore, this review evaluated the mental health status who complained a small penis examined state nonsurgical surgical treatments for male patients seeking penile enlargement, along risk assessment each. Various medical, tractional, injectable, modalities have been investigated augmentation. However, development new products techniques, such as Penuma, tissue engineering may yield fewer...
Objective: To elaborate the specific mechanism of chestnut polysaccharides beneficial to spermatogenesis. Design: 16s rRNA-based study Setting and population: Busulfan treatment mouse Methods: Intestinal microbiota different groups (n = 10) were characterized by 16S rRNA gene sequencing V3-V4 region. Hematoxylin eosin staining (H&E), Immunochemistry (IHC) Real-time fluorescence quantitative PCR (RT-qPCR) acted as methods detect expression changes in tissue. Main outcome measure:...
Abstract Background Human sperm concentration and motility have dropped dramatically (50%) in the past few decades, environmental factors are involved this decline. Long non‐coding RNAs (lncRNA) been discovered to be many cellular processes including spermatogenesis. Objective This investigation aimed explore role of lncRNA8276 murine Materials methods The expression was modified by knockdown or overexpression mouse testes spermatogonial stem cells (C18‐4 cell line). Sperm quality determined...
Abstract Long terminal repeat (LTR) retrotransposons are the dominant feature of higher plant genomes, which have a similar life cycle with retrovirus. Previous studies cannot account for all observed complex LTR retrotransposon patterns. In this study, we first identified 63 in rice genome, and most elements harbored flanking target-site duplications (TSDs). But these outermost LTRs had not highly homologous can’t be explained. We propose new model that recombination two different normal...
Human sperm concentration and motility have dropped dramatically (50%) in the past few decades, environmental factors are involved this decline. Long non-coding RNAs (lncRNA) been discovered to be many cellular processes such as spermatogenesis. This investigation aimed explore role of lncRNA8276 mice spermatogenesis.The expression was modified by knockdown or overexpression mouse testes spermatogonial stem cells (C18-4 cell line). The quality determined F0 F1 generational mice. And...
Abstract Background: Our previous study confirmed the beneficial effects of chestnut polysaccharides on spermatogenesis process, but exact mechanism is not clear. Several studies have demonstrated importance a balanced gut microbiota in maintaining normal reproductive function. In this study, we investigated biological functions from perspective function, expecting to elaborate specific spermatogenesis. Results: Compared with Vehicle group, germ cell quantity, intestinal structure and tight...
Background: High fat diet (HFD)-related male infertility is a growing issue worldwide, and it crucial to overcome this problem ameliorate the distress of infertile couples. The aim investigation was designed explore role alginate oligosaccharide (AOS)-benefited gut microbiota in rescuing HFD-impaired spermatogenesis fertility.Methods: Faecal microbes derived from AOS dosed mice (A10-FMT) were transplanted fed with HFD. sperm concentration motility, pregnant rate, number pup/litter, microbes,...