A5064756948- Epigenetics and DNA Methylation
- Reproductive Biology and Fertility
- Birth, Development, and Health
- Genetic Syndromes and Imprinting
- Ovarian function and disorders
- Prenatal Screening and Diagnostics
- Reproductive Health and Technologies
- Gestational Diabetes Research and Management
- Microtubule and mitosis dynamics
- Sperm and Testicular Function
- Tea Polyphenols and Effects
- Phytoestrogen effects and research
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive System and Pregnancy
- Cancer-related gene regulation
- Photosynthetic Processes and Mechanisms
- Autophagy in Disease and Therapy
- Gestational Trophoblastic Disease Studies
- Effects and risks of endocrine disrupting chemicals
- Mitochondrial Function and Pathology
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- Plant Molecular Biology Research
- Light effects on plants
- Nanoparticles: synthesis and applications
Qingdao Agricultural University
2016-2025
McGill University Health Centre
2018-2024
North Carolina State University
2019
Henan Provincial People's Hospital
2013-2015
Chinese Academy of Sciences
2011-2015
Institute of Zoology
2012-2014
Zhengzhou University
2014
University of Chinese Academy of Sciences
2013
Background: Maternal obesity has adverse effects on oocyte quality, embryo development, and the health of offspring.Objectives: To understand underlying mechanisms responsible for negative maternal obesity, we investigated DNA methylation status several imprinted genes metabolism-related genes.Methods: Using a high-fat-diet (HFD)-induced mouse model analyzed in oocytes from control obese dams liver their offspring. Analysis was performed using combined bisulfite restriction analysis (COBRA)...
In animals, mtDNA is always transmitted through the female and this termed “maternal inheritance.” Recently, autophagy was reported to be involved in maternal inheritance by elimination of paternal mitochondria Caenorhabditis elegans ; moreover, immunofluorescence, P62 LC3 proteins were also found colocalize sperm after fertilization mice. Thus, it has been speculated that may an evolutionary conserved mechanism for mitochondrial elimination. However, using two transgenic mouse strains, one...
The duration of a woman's reproductive period is determined by the size and persistence dormant oocyte pool. Specific genes are essential for follicle maintenance female fertility. mechanisms that regulate expression these poorly understood. We found cullin-ring finger ligase-4 (CRL4) complex was crucial in this process. Oocyte-specific deletion CRL4 linker protein DDB1 or its substrate adaptor VPRBP (also known as DCAF1) caused rapid loss, premature ovarian insufficiency, silencing...
Androgenetic complete hydatidiform moles are human pregnancies with no embryos and affect 1 in every 1,400 pregnancies. They have mostly androgenetic monospermic genomes all the chromosomes originating from a haploid sperm maternal chromosomes. were described 1977, but how they occur has remained an open question. We identified bi-allelic deleterious mutations MEI1, TOP6BL/C11orf80, REC114, roles meiotic double-strand breaks formation women recurrent moles. investigated occurrence of...
Maternal diabetes has adverse effects not only on oocyte quality but also embryo development. However, it is still unknown whether the DNA imprinting in oocytes altered by diabetes. By using streptozotocin (STZ)-induced and nonobese diabetic (NOD) mouse models we investigated effect of maternal methylation imprinted genes oocytes. Mice which were judged as being 4 days after STZ injection used for experiments. In superovulated mice, pattern Peg3 differential regions (DMR) was affected a...
The adverse effects on offspring of diabetic and/or obese mothers can be passed to the next generation. However, mechanisms behind this are still unclear. Epigenetics may play a key role during process.To confirm hypothesis, we investigated DNA methylation several imprinted genes in spermatozoa from utilizing streptozotocin (STZ)- and high-fat-diet (HFD)-induced mouse models.We found that Peg3 was significantly increased compared normal mothers. H19 higher than non-diabetic mothers.These...
Abstract Background Undernourishment in utero has deleterious effects on the metabolism of offspring, but mechanism transgenerational transmission metabolic disorders is not well known. In present study, we found that undernourishment resulted female F1 and F2 mouse model. Results Undernutrition induced females, which was transmitted to females. The global methylation oocytes exposed undernutrition decreased compared with control. KEGG analysis showed genes differential regions (DMRs)...
Gestational diabetes mellitus (GDM), a common pregnancy disease, has long-term negative effects on offspring health. Epigenetic changes may have important contributions to that, but the underlying mechanisms are not well understood. Here, we report influence of GDM DNA methylation (GDF1) oocytes and possible mechanisms. Our results show that induces genomic hyper-methylation oocytes, at least part altered is inherited by F2 which be reason for inheritance metabolic disorders. We further find...
Chromosome segregation in mammalian oocyte meiosis is an error-prone process, and any mistake this process may result aneuploidy, which the main cause of infertility, abortion many genetic diseases. It now well known that shugoshin protein phosphatase 2A (PP2A) play important roles protection centromeric cohesion during first meiosis. PP2A can antagonize phosphorylation rec8, a member cohesin complex, at centromeres thus prevent cleavage rec8 so maintain chromatids. SETβ physically interacts...
The adverse effects of maternal diabetes on oocyte maturation and embryo development have been reported.In this study, we used time-lapse live cell imaging confocal microscopy to investigate the dynamic changes ER ER's structural dynamics during maturation, fertilization early development.We report that first became remodeled into a dense ring around developing MI spindle, then surrounded spindle migration cortex. reorganization mouse was characterized by striking localization pronuclei in...
What are the effects of high-glucose concentrations on DNA methylation human oocytes?High-glucose altered levels Peg3 and Adiponectin in vitro maturation oocytes.Maternal diabetes has a detrimental influence oocyte quality including epigenetic modifications, as shown non-human mammalian species.Immature metaphase I (MI) stage oocytes good were retrieved from patients who had normal ovarian potential underwent ICSI Reproductive Medicine Center People's Hospital Zhengzhou University. MI...
For humans, ARTs (assisted reproductive technologies) have become the most effective method to treat subfertility/infertility in clinic. To obtain enough oocytes during ART, ovarian stimulation is performed by exogenous hormones, and some patients undergo several cycles. Although adverse effects of on women offspring are reported, few studies focused multiple superovulation reserve. In present study, we found that repeated significantly reduced primordial follicle number serum AMH. Compared...
We explored the effects of different doses letrozole on incidence ovarian hyperstimulation syndrome (OHSS) after oocyte retrieval during in vitro fertilization (IVF) patients with high-risk OHSS. A total 88 were randomly divided into a control group, and groups treated 2.5 mg, 5 or 7.5 mg letrozole. found that from fifth day human chorionic gonadotrophin (hCG) treatment, E2 level decreased there statistical differences between four (p < 0.05). From eighth hCG luteinizing hormone (LH)...
The global prevalence of weight loss is increasing, especially in young women. However, the extent and mechanisms by which maternal affects offspring still poorly understood. Here, using an enriched environment (EE)-induced model, we show that improves general health reprograms metabolic gene expression mouse offspring, epigenetic alterations can be inherited for at least two generations. EE mothers induced its associated physiological changes such as decreased adiposity improved glucose...
Maternal diabetes compromises the quality and developmental potential of oocytes. Therefore, it is important to study how ameliorate adverse effects on oocyte quality. Epigallocatechin gallate (EGCG) has a variety physiological activities, including anti-inflammatory, antioxidant, anti-diabetes. In present study, we evaluated effect EGCG maturation diabetic oocytes in vitro.
Background Vaginitis is a common infection in women, with approximately 75% of women experiencing at least one episode during their lifetime. Although antimicrobial agents are widely used to treat vaginitis, recurrent vaginitis occurs some patients. Resistance these the major cause vaginitis. Therefore, there an urgent need develop novel drugs. Methods We investigated efficacy new biological bacteriostatic agent (BBA), composed lysozyme, phytoalexin, chitosan oligosaccharide, sinensetin,...
Abstract Controlled ovarian stimulation by exogenous gonadotrophins is a key procedure during the in vitro fertilization cycle to obtain sufficient number of oocytes humans. Previous studies demonstrated that repeated superovulation had deleterious effects on ovaries. However, whether adversely affects mitochondrial functions cumulus cells remains unclear. In this study, mice were divided into three groups: once (R1); times (R3), and five (R5). We evaluated DNA copies (mtDNA) observed...
It is demonstrated that repeated superovulation has deleterious effects on mouse ovaries and cumulus cells. However, little known about the of early embryos. Epigenetic reprogramming an important event in embryonic development could be easily disrupted by environment. Thus, we speculated multiple superovulations may have adverse histone modifications Female CD1 mice were randomly divided into four groups: (a) spontaneous estrus cycle (R0); (b) with once (R1); (c) three times at a 7-day...
Maternal diabetes mellitus reduces oocyte quality, such as abnormalities of spindle assembly and chromosome segregation, mitochondrial dysfunction, decrease fertilization rate, increase ROS, so on. So, it is important to research how restore the decreased quality induced by maternal mellitus. Polyphenols are most abundant bioactive components green tea. It reported that tea polyphenols have many health functions, for instance anti-oxidation, anti-inflammation, anti-obesity, anti-diabetes....
Maternal diabetes mellitus not only has severe deleterious effects on fetal development, but also it affects transmission to the next generation. However, underlying mechanisms for these are still clear.We investigated methylation patterns and expressions of imprinted genes Peg3, Snrpn, H19 in mid-gestational placental tissues whole fetus utilizing streptozotocin (STZ)-induced hyperglycemic mouse model quantitative analysis by PCR real-time PCR. The protein expression Peg3 was evaluated...