A5102774009- Reproductive Biology and Fertility
- Microtubule and mitosis dynamics
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Pluripotent Stem Cells Research
- Sperm and Testicular Function
- Genomics and Chromatin Dynamics
- Prenatal Screening and Diagnostics
- DNA Repair Mechanisms
- Genetic Syndromes and Imprinting
- Plant Molecular Biology Research
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Animal Genetics and Reproduction
- Renal and related cancers
- Ovarian function and disorders
- Acupuncture Treatment Research Studies
- Tissue Engineering and Regenerative Medicine
- Adipose Tissue and Metabolism
- Kruppel-like factors research
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
Shandong University of Traditional Chinese Medicine
2021-2024
Tianjin Medical University
2023-2024
Institute of Endocrinology
2024
Zhujiang Hospital
2022-2024
Southern Medical University
2022-2024
Institute of Zoology
2014-2023
Chinese Academy of Sciences
2014-2023
The Affiliated Yongchuan Hospital of Chongqing Medical University
2017-2023
Chongqing Medical University
2017-2023
Yunnan University
2004-2023
The global prevalence of prediabetes and type 2 diabetes (T2D) is increasing, it contributing to the susceptibility its related epidemic in offspring. Although impacts paternal impaired fasting blood glucose intolerance on metabolism offspring have been well established, exact molecular mechanistic basis that mediates these remains largely unclear. Here we show increases through gametic epigenetic alterations. In our findings, led insulin resistance Relative controls, prediabetic fathers...
Background: Maternal obesity has adverse effects on oocyte quality, embryo development, and the health of offspring.Objectives: To understand underlying mechanisms responsible for negative maternal obesity, we investigated DNA methylation status several imprinted genes metabolism-related genes.Methods: Using a high-fat-diet (HFD)-induced mouse model analyzed in oocytes from control obese dams liver their offspring. Analysis was performed using combined bisulfite restriction analysis (COBRA)...
In animals, mtDNA is always transmitted through the female and this termed “maternal inheritance.” Recently, autophagy was reported to be involved in maternal inheritance by elimination of paternal mitochondria Caenorhabditis elegans ; moreover, immunofluorescence, P62 LC3 proteins were also found colocalize sperm after fertilization mice. Thus, it has been speculated that may an evolutionary conserved mechanism for mitochondrial elimination. However, using two transgenic mouse strains, one...
Folium Mori, the leaf of Morus alba L. (Moraceae), has been used in traditional Chinese medicine (TCM) for treating diabetes. However, it is unclear which components mulberry are effective treatment type 2 diabetes mellitus (T2DM).To investigate flavonoids and polyphenols leaves their antihyperglycemic antihyperlipidemic effects T2DM rats.Male Sprague-Dawley rats were divided into five groups: normal control (NC), diabetic (DBC), group with 0.3 mg/kg b.w./day rosiglitazone (RSG), 7 g/kg TCM...
Abstract The COVID‐19 pandemic has taken a significant toll on people worldwide, and there are currently no specific antivirus drugs or vaccines. Herein it is therapeutic based catalase, an antioxidant enzyme that can effectively breakdown hydrogen peroxide minimize the downstream reactive oxygen species, which excessively produced resulting from infection inflammatory process, reported. Catalase assists to regulate production of cytokines, protect oxidative injury, repress replication...
Abstract Sperm‐induced Ca 2+ rise is critical for driving oocyte activation and subsequent embryonic development, but little known about how lasting oscillations are regulated. Here it shown that NLRP14, a maternal effect factor, essential keeping early development. Few embryos lacking NLRP14 can develop beyond the 2‐cell stage. The impaired developmental potential of Nlrp14 ‐deficient oocytes mainly caused by disrupted cytoplasmic function calcium homeostasis due to altered mitochondrial...
Abstract This study was conducted to establish embryonic stem (ES) cell lines from porcine blastocysts. Blastocysts were collected China miniature pigs at day 7–9 of pregnancy. Embryos either directly (intact embryos) cultured on mitomysin C‐inactivated murine fibroblasts (MEF) as feeder layers, or used isolate the inner masses (ICM) by enzyme digestive method and then cultured. It found that could ICMs without any damages cells in all blastocysts (28). All attached layers. Primary colonies...
During mouse antral follicle development, the oocyte chromatin gradually transforms from a less condensed state with no Hoechst-positive rim surrounding nucleolus (NSN) to fully (SN). Compared SN oocytes, NSN oocytes display higher gene transcription activity and lower rate of meiosis resumption (G2/M transition), they are mostly arrested at two-cell stage after in vitro fertilization. To explore differences between maternal factors required for developmental competence, we compared...
Recently, numerous studies have reported that the mature sperm contains both coding and non-coding RNAs delivers some to oocyte at fertilization. However, functions of carried by fertilization in embryonic development remains a mystery. In this study, spermatozoa were treated with lysolecithin, pronase RNases (RNase A RNase H) remove sperm-carried RNAs, then injected into normal oocyte. The results showed after treatment, content was decreased about 90%. blastocyst formation rate live birth...
This study was conducted to examine expression of centromere protein B (CENPB), spindle checkpoint MAD2 (mitotic arrest deficient protein), and antiapoptotic BCL2; activities MAPK (mitogen-activated kinase) mitochondria distribution in pig oocytes during aging, their relationship with sister chromatid separation meiosis II embryo fragmentation apoptosis after activation. After immature were cultured for 40–72 h, CENPB, MAD2, tubulin, BCL2, the examined by immunoblotting. Spindles,...
Abstract Genomic imprinting plays a very important role during development and its abnormality may heavily undermine the developmental potential of bovine embryos. Because limited resources cow genome, genomic imprinting, both in normal somatic cell nuclear transfer (SCNT) cloning, is not well documented. DNA methylation thought to be major factor for establishment imprinting. In our study, we determined status differential methylated regions (DMRs) four imprinted genes spontaneously aborted...
Errors in chromosome segregation or distribution may result aneuploid embryo formation, which causes implantation failure, spontaneous abortion, genetic diseases, death. Embryonic aneuploidy occurs when aberrations are present gametes early embryos. To date, it is still unclear whether the spindle assembly checkpoint (SAC) required for regulation of mitotic cell cycle progression to ensure fidelity during preimplantation development. In this study, using overexpression and RNA interference...
Both endogenous and exogenous factors can induce DNA double-strand breaks (DSBs) in oocytes, which is a potential risk for human-assisted reproductive technology as well animal nuclear transfer. Here we used bleomycin (BLM) laser micro-beam dissection (LMD) to DSBs germinal vesicle (GV) stage oocytes compared the breakdown (GVBD) rates first polar body extrusion (PBE) between DSB untreated oocytes. Employing live cell imaging immunofluorescence labeling, observed dynamics of fragments during...
The adverse effects on offspring of diabetic and/or obese mothers can be passed to the next generation. However, mechanisms behind this are still unclear. Epigenetics may play a key role during process.To confirm hypothesis, we investigated DNA methylation several imprinted genes in spermatozoa from utilizing streptozotocin (STZ)- and high-fat-diet (HFD)-induced mouse models.We found that Peg3 was significantly increased compared normal mothers. H19 higher than non-diabetic mothers.These...
The quality of post-ovulatory oocytes decreases with aging. In this study, we aimed to investigate the effects N-acetyl-L-cysteine (NAC), a broadly used antioxidant, on oocyte in mouse aging vitro. NAC at 0.6mM concentration was added culture medium (M2), and analyzed 6h, 12h, 18h 24h culture. We found that frequency spindle defects decreased NAC-treated compared those without treatment. treatment significantly abnormal distribution cortical granules (CGs) during for 24h. Decreased...
Panax notoginseng saponins ( PNS ) are a commonly used traditional medicine to treat diabetes in China. Recent studies have confirmed their anti‐diabetic effects, but the underlying mechanisms remained unclear. The present study was designed explore whether decrease hyperglycemia by improving insulin sensitivity skeletal muscle and elucidate molecular mechanisms. effects of were analyzed myoblast cell line, C2C12, high fat diet‐induced diabetic KKA y mice. C2C12 cells treated with (50, 100,...
Meiosis initiation is a crucial step for the production of haploid gametes, which occurs from anterior to posterior in fetal ovaries. The asynchrony transition mitosis meiosis results heterogeneity female germ cell populations, limits studies and progression at higher resolution level. To dissect process initiation, we investigated transcriptional profiles 19 363 single cells collected E12.5, E14.5, E16.5 mouse Clustering analysis identified seven groups defined dozens corresponding...
Abstract The formation of zygote is the beginning mammalian life, and dynamic epigenetic modifications are essential for normal development. H3K27 di-methylation (H3K27me2) tri-methylation (H3K27me3) marks facultative heterochromatin which maintains transcriptional repression established during early development in many eukaryotes. However, mechanism underlying establishment regulation asymmetry remains obscure. Here we show that maternal EZH2 required H3K27me3 mouse zygotes. combined...
AbstractIt is well known that MAPK plays pivotal roles in oocyte maturation, but the function of MEK (MAPK kinase) remains unknown. We have studied expression, subcellular localization and functional during meiotic maturation mouse oocytes. found MEK1/2 phoshorylation (p-MEK1/2, indicative activation) was low GV (germinal vesicle) stage, increased 2h after GVBD vesicle breakdown), reached maximum at metaphase II. Secondly, we P-MEK1/2 restricted prior to GVBD. In prometaphase I I, p-MEK1/2...
The behavior of mitochondria during early oogenesis remains largely unknown in zebrafish. We used three mitochondrial probes (Mito Tracker Red CMXRos, Mito Green FM, and JC-1) to stain zebrafish oocyte mitochondria, confocal microscopy analyze aggregation distribution. By using fluorescence recovery after photobleaching (FRAP), we traced movement. microtubule assembly inhibitor nocodazole microfilament cytochalasin B (CB) were the role microtubules microfilaments on dual emission probe,...
GM130, a cis-Golgi protein, plays key roles in various mitotic events, but its function mammalian oocyte meiosis remains unknown. In this study, we found that GM130 was localized to the spindle poles at both metaphase I and II stages associated with midbody telophase stage. The association of further confirmed by colocalization centrosome-associated proteins, MEK1/2. By nocodazole treatment, clarified localization consistently dependent on assembly. Then investigated possible specific...
Mammalian oocyte maturation is distinguished by highly asymmetric meiotic divisions during which a haploid female gamete produced and almost all the cytoplasm maintained in egg for embryo development. Actin-dependent meiosis I spindle positioning to cortex induces formation of polarized actin cap polarity, it determines resulting two polar bodies. Here we investigate functions Cdc42 oocyte-specific deletion through Cre-loxP conditional knockout technology. We find that causes infertility...