A5100361476- Reproductive Biology and Fertility
- Ecology and Vegetation Dynamics Studies
- Microtubule and mitosis dynamics
- Sperm and Testicular Function
- Genomics and Chromatin Dynamics
- Species Distribution and Climate Change
- Plant and animal studies
- Land Use and Ecosystem Services
- Estrogen and related hormone effects
- Plant Parasitism and Resistance
- Wildlife Ecology and Conservation
- DNA Repair Mechanisms
- Hydrology and Watershed Management Studies
- Advanced Aircraft Design and Technologies
- Soil Carbon and Nitrogen Dynamics
- Aerodynamics and Fluid Dynamics Research
- Pluripotent Stem Cells Research
- Chromosomal and Genetic Variations
- Plant Water Relations and Carbon Dynamics
- Rangeland Management and Livestock Ecology
- Aerospace and Aviation Technology
- Ovarian function and disorders
- Epigenetics and DNA Methylation
- Hydrological Forecasting Using AI
- Coastal wetland ecosystem dynamics
Chengdu Research Base of Giant Panda Breeding
2019-2024
Northeast Agricultural University
2024
Chinese Academy of Sciences
2007-2022
Chengdu Institute of Biology
2015-2022
South China Agricultural University
2022
Beijing Institute of Technology
2022
Huazhong University of Science and Technology
2019-2020
University of Chinese Academy of Sciences
2015-2018
CAS Key Laboratory of Urban Pollutant Conversion
2015
University of Science and Technology of China
2015
Recently, a novel variant of ER-alpha, ER-alpha36 was identified and cloned. lacks intrinsic transcription activity mainly mediates nongenomic estrogen signaling. Here, we studied the role signaling pathways mediated by in tamoxifen resistance agonist action.The cellular localization examined immunofluorescence MCF-7 cells Hec1A cells. breast cancer cells, expressing recombinant (MCF-7/ER36), endometrial with siRNA knockdown (Hec1A/RNAiER36) were treated 17beta-estradial (E2) (TAM) absence...
In mitosis, the spindle assembly checkpoint (SAC) prevents anaphase onset until all chromosomes have been attached to microtubules and aligned correctly at equatorial metaphase plate. The major proteins in mitosis consist of mitotic arrest-deficient (Mad)1-3, budding uninhibited by benzimidazole (Bub)1, Bub3, monopolar 1(Mps1). During meiosis, for formation a haploid gamete, two consecutive rounds chromosome segregation occur with only one round DNA replication. To pull homologous opposite...
During mouse antral follicle development, the oocyte chromatin gradually transforms from a less condensed state with no Hoechst-positive rim surrounding nucleolus (NSN) to fully (SN). Compared SN oocytes, NSN oocytes display higher gene transcription activity and lower rate of meiosis resumption (G2/M transition), they are mostly arrested at two-cell stage after in vitro fertilization. To explore differences between maternal factors required for developmental competence, we compared...
Errors in chromosome segregation or distribution may result aneuploid embryo formation, which causes implantation failure, spontaneous abortion, genetic diseases, death. Embryonic aneuploidy occurs when aberrations are present gametes early embryos. To date, it is still unclear whether the spindle assembly checkpoint (SAC) required for regulation of mitotic cell cycle progression to ensure fidelity during preimplantation development. In this study, using overexpression and RNA interference...
Abstract Predicting the impact of grazing on vegetation structure, and thus ecosystem services, is one main technical bottlenecks in alpine grassland conservation management. However, past researches based fixed, discrete, qualitative indicators limited our understanding for effect plant community assembly. Research quantitative competitor, stress tolerator, ruderal (CSR) theory increases possibilities analyzing this ecological process. To test hypothesis that dominant strategy meadow will...
P38αMAPK (p38α) is usually activated in response to various stresses and plays a role the inhibition of cell proliferation tumor progression, but little known about its roles meiotic spindle assembly. In this study, we characterized dynamic localization p38α explored function mouse oocyte maturation. P38α specifically colocalized with γ-tubulin Plk1 at center MTOCs poles. Depletion by specific morpholino injection resulted severely defective spindles misaligned chromosomes probably via MK2...
Climate and human-driven changes play an important role in regional droughts. Northwest Yunnan Province is a key region for biodiversity conservation China, it has experienced severe droughts since the beginning of this century; however, extent contributions from climate remains unclear. We calculated ecosystem evapotranspiration (ET) water yield (WY) northwest Province, China 2001 to 2013 using meteorological remote sensing observation data Surface Energy Balance System (SEBS) model....
The role of androgen and receptors (ARs) in males has been well established. This steroid its receptor also exist follicles, but their functions are still unclear. In this study, using a culture system containing low dose hypoxanthine, we revealed the positive contribution testosterone to oocyte meiotic resumption. By performing ultracentrifugation allow clear visualization porcine germinal vesicles, our results provide evidence that mitogen-activated protein kinase (MAPK) itself not cumulus...
ERK3 (extracellular signal-regulated kinase 3) is an atypical member of the mitogen-activated protein (MAP) family serine/threonine kinases. Little known about its function in mitosis, and even less roles mammalian oocyte meiosis. In present study, we examined localization, expression functions during mouse meiotic maturation. Immunofluorescent analysis showed that localized to spindles from pre-MI stage MII stage. co-localized with α-tubulin on spindle fibers asters oocytes after taxol...
In mouse ovarian follicles, granulosa cells but not oocytes take up glucose to provide the oocyte with nourishments for energy metabolism. Diabetes-induced hyperglycemia or absorption inefficiency consistently causes cell apoptosis and further exerts a series of negative impacts on including reduced meiosis resumption rate, low quality preimplantation embryo degeneration. Here we compared transcriptome from genetically derived NOD diabetic mice chemically induced STZ that corresponding...
MAPK-activated protein kinase 2 (MK2), a direct substrate of p38 MAPK, plays key roles in multiple physiological functions mitosis. Here, we show for the first time unique distribution pattern MK2 meiosis. Phospho-MK2 was localized on bipolar spindle minus ends and along interstitial axes homologous chromosomes extending over centromere regions arm at metaphase meiosis (MI stage) mouse oocytes. At second (MII stage), p-MK2 inner region sister chromatids as dots. Knockdown or inhibition...
Currently, nearly 70% of giant panda populations are facing survival challenges. The introduction wild individuals can bring vitality to them. To explore this possibility, we hypothetically introduced pandas from Tangjiahe and Wanglang into Liziping Daxiangling Nature Reserves. We collected feces these areas analyzed the genetic diversity population viability before after using nine microsatellite loci. results showed level large were better than small populations. investigated effects time...
In a quantum key distribution (QKD) system, the error rate needs to be estimated for determining joint probability between legitimate parties, and improving performance of reconciliation. We propose an efficient estimation scheme QKD, which is called parity comparison method (PCM). proposed method, group sifted keys practically analysed estimate bit instead using traditional sampling. From simulation results, evidently improves accuracy decreases revealed information in most realistic...
Abstract The different responses of plant species to resource stress are keys understand the dynamics community in a changing environment. To test hypothesis that nitrogen (N) increase would benefit N competitive species, rather than stress-tolerant compete with neighbours, we conducted an experiment neighbour removal, addition and soil moisture as treatments alpine grassland on southeastern Tibetan Plateau. Both growths competitive-response abilities (CRA, ability tolerate inhibitory...
Human activities change the natural ecosystem and cause decline of intact ecosystem. Establishing an applicable efficient human activity monitoring indicator system benefits China’s ambitious national park construction. In this study, we established a refined technique for ecological intactness scores (EIS) applied it in area Giant Panda National Park (GPNP) from 1980 to 2020 by quantifying four types interferences including land use cover (LUCC), road construction, water reservoir...
There is no knowledge of winter plant phenology and its controlling factors on the Qinghai-Tibetan Plateau (QTP). Thus, we conducted a 4 year growth dynamics study in alpine meadow eastern QTP.From November 2013 to March 2017, 'winter-growth' 'winter-green' species was recorded every 5 d. In November-February from 2014 2015, above-ground biomass (AGB) random plots calculated distinguish different patterns among growing species. The percentage abundance relative summer population for forbs...
Abstract It is very important for water resource management to analyse the change rule of runoff. In this paper, a segmented Mann-Kendall trend analysis method, which called M-K method in following, proposed periods significant within time series. This fills gap that changes internal long sequence are ignored test, by finding out period may be new runoff changes. Fuhe River, one main rivers Poyang Lake Yangtze River basin, taken example. Based on data Lijiadu section Fu Basin from 1957 2015,...
Abstract The stress gradient hypothesis (SGH) proposes that the frequency of positive interactions among plants is increased with stress. However, in alpine meadow ecosystems, joint effects herbivore grazing and resource on plant–plant remain poorly understood. By investigating spatial associations two dominant, widely distributed species—Carex praeclara (unpalatable) Carum carvi (palatable)—we explored changes at three desertification levels (light, moderate severe) under grazed ungrazed...