A5101845595- Effects and risks of endocrine disrupting chemicals
- Reproductive Biology and Fertility
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- RNA Research and Splicing
- RNA modifications and cancer
- Estrogen and related hormone effects
- Chromosomal and Genetic Variations
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Toxic Organic Pollutants Impact
- Dengue and Mosquito Control Research
- Thyroid Disorders and Treatments
- Ovarian function and disorders
- Sperm and Testicular Function
- Cancer-related gene regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Geology and Paleoclimatology Research
- Insect symbiosis and bacterial influences
- Animal Genetics and Reproduction
- Cancer-related molecular mechanisms research
- Prenatal Screening and Diagnostics
- Metalloenzymes and iron-sulfur proteins
University of Chinese Academy of Sciences
2016-2025
Research Center for Eco-Environmental Sciences
2015-2025
Hubei University of Chinese Medicine
2025
Children's Hospital of Chongqing Medical University
2025
Chongqing Medical University
2025
Chongqing Maternal and Child Health Hospital
2025
Ministry of Agriculture and Rural Affairs
2025
Chinese Academy of Agricultural Engineering
2025
Chinese Academy of Sciences
2015-2024
Southern Medical University
2023-2024
Spermiogenesis is a critical, post-meiotic phase of male gametogenesis, in which the proper gene expression essential for sperm maturation. However, underFlying molecular mechanism that controls mRNA round spermatids remains elusive. Here, we identify FBXO24, an orphan F-box protein, highly expressed testis humans and mice interacts with splicing factors (SRSF2, SRSF3, SRSF9) to modulate alternative spermatids. Genetic mutation FBXO24 causes many abnormal events spermatids, thus affecting...
Peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) has been shown to influence energy metabolism. Hence, we explored a strategy target PGC-1α expression treat metabolic syndromes. We developed high-throughput screening assay that uses the human promoter drive of luciferase. The effects lead compound stimulation on in muscle cells and hepatocytes were investigated vitro vivo. A novel small molecule, ZLN005, led changes mRNA levels, glucose uptake, fatty acid oxidation L6...
Recently, numerous studies have reported that the mature sperm contains both coding and non-coding RNAs delivers some to oocyte at fertilization. However, functions of carried by fertilization in embryonic development remains a mystery. In this study, spermatozoa were treated with lysolecithin, pronase RNases (RNase A RNase H) remove sperm-carried RNAs, then injected into normal oocyte. The results showed after treatment, content was decreased about 90%. blastocyst formation rate live birth...
Polycystic ovary syndrome (PCOS) is an endocrine disorder characterized by abnormal hormone levels in peripheral blood and poor-quality oocytes. PCOS a pathophysiological caused chronic inflammation oxidative stress. The aim of this study was to investigate the mechanism melatonin regulation on androgen production antioxidative damage granulosa cells from patients with hypoestrogenia hyperandrogenia. Cumulus-oocyte complexes were collected who had low estrogen follicular fluids. Melatonin...
Fungal epidithiodiketopiperazines (ETPs) possess large structural diversity and complexity due to modifications of the cyclodipeptide skeleton. Elucidation biosynthetic pathway pretrichodermamide A (1) in Trichoderma hypoxylon revealed a flexible catalytic machinery multiple enzymes for generating ETP diversity. Seven tailoring encoded by tda cluster are involved 1 biosynthesis, that is, four P450s TdaB TdaQ 1,2-oxazine formation, TdaI C7'-hydroxylation, TdaG C4, C5-epoxidation, two...
Data concerning effects of tetrabromobisphenol A (TBBPA) on thyroid hormone (TH)-dependent vertebrate development have been limited, although TBBPA has demonstrated in vitro to disrupt the TH signaling pathway at transcriptional level. In this study, we investigated T3-induced and spontaneous Xenopus laevis metamorphosis, which share many similarities with TH-dependent higher vertebrates. a 6-day metamorphosis assay using premetamorphic tadpoles, 10–1000 nM exhibited inhibitory expression...
Airway epithelial cells constitute the first line of defense against external noxious stimuli and play crucial roles in release inflammatory cytokines (IL33, IL25 TSLP), initiating airway allergic diseases such as asthma. IL33 plays critical physiological processes T2-endotype However, mechanisms by which allergen exposure triggers from remain unclear. Integrated bioinformatic analysis transcriptional bulk RNA-seq single cell (scRNA-seq) data were used to identify core genes determine...
To observe the effect of electroacupuncture (EA) on basic fibroblast growth factor (BFGF)/glycogen synthase kinase-3β (GSK-3β) pathway in Parkinson's disease (PD) mice, so as to explore potential mechanisms EA treating PD. Fifty C57/BL6 male mice were randomly divided into control, model, EA, inhibitor and EA+inhibitor (combination) groups, with 10 each group. The PD mouse model was established by intraperitoneal injection 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine. Mice group received...
Yunnan Province is located in southwestern China and neighbors the Southeast Asian countries, all of which are dengue-endemic areas. In 2000-2013, sporadic imported cases dengue fever (DF) were reported almost annually Province. During 2013-2015, we confirmed that a large-scale indigenous DF outbreak emerged cities near China-Myanmar-Laos border.Epidemiological characteristics during 2013-2015 evaluated by retrospective analysis. A total 232 virus (DENV)-positive sera randomly collected for...
The safety of bisphenol A (BPA) alternatives has attracted much attention due to their wide use. In this study, we investigated the effects F (BPF), an alternative BPA, on thyroid hormone (TH) signaling and postembryonic development in vertebrates using T3-induced spontaneous Xenopus metamorphosis as models. We found that assay, higher concentrations BPF (100-10000 nM) antagonized TH-response gene transcription morphological changes including intestinal remodeling a concentration-dependent...
Continuous vector pathogen surveillance is essential for preventing outbreaks of mosquito-borne diseases. Several mosquito species acting as vectors Japanese encephalitis virus (JEV), dengue virus, Zika malaria parasites and other pathogens are primary in Shanghai, China. However, few surveys human pathogenic arboviruses mosquitoes Shanghai have been reported the last ten years. Therefore, this study, we evaluated activity China during 2016 tested presence alphaviruses, flaviviruses,...
Abstract Background RNA splicing plays significant roles in fundamental biological activities. However, our knowledge about the of alternative and underlying mechanisms during spermatogenesis is limited. Results Here, we report that Serine/arginine-rich factor 2 (SRSF2), also known as SC35, critical male reproduction. Male germ cell-specific deletion Srsf2 by Stra8-Cre caused complete infertility defective spermatogenesis. Further analyses revealed disrupted differentiation meiosis...
In August 2013, Xishuangbanna, Yunnan Province, China, had its first dengue outbreak. Dengue virus (DENV) RNA detection in sera or viral isolates revealed that all 222 autochthonous patients detected and three Chinese travelers from Laos (imported cases) were positive for DENV-3 serotype, while DENV-1 DENV-4 Myanmar Thailand during the For 33 suspected cases collected before outbreak, two imported nine residents living (Laotian DENV-3. Further, a random subset of was sequenced full envelope...
With increases in global travel and trade, the spread of arboviruses is undoubtedly alarming. Pathogen detection field-caught mosquitoes can provide earliest possible warning transmission. Insect-specific flavivirus (ISFV) has been first detected 1991 documented worldwide latest ten years. Although infection with ISFVs apparently limited to insects, an increase rate mosquito-borne flaviviruses may be able induce cytopathic effects vertebrate cells during co-infection other human pathogens....
Acute myocarditis is an uncommon and potentially life-threatening disease. Scoring systems are essential for predicting outcome evaluating the therapy effect of adult patients with acute myocarditis. The aim this study was to determine value Sequential Organ Failure Assessment (SOFA), Physiology Chronic Health Evaluation IV (APACHE IV) second Simplified Score (SAPS II) scoring in short-term mortality these patients. We retrospectively analyzed data from 305 suffering between April 2005...
The increasing trend of adult cases Japanese encephalitis (JE) in China, particularly northern has become an important public health issue. We conducted epidemiological investigation the south Shanxi Province to examine relationships between mosquitoes, virus (JEV), and JE cases.Mosquito specimens were collected from courtyards farmers' households pig farms Province. Mosquitoes pooled, homogenized, centrifuged. Reverse transcription-polymerase chain reaction (RT-PCR) was used detect...