A5005979529- Reproductive Biology and Fertility
- Ovarian function and disorders
- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Uterine Myomas and Treatments
- Liver Disease Diagnosis and Treatment
- Assisted Reproductive Technology and Twin Pregnancy
- Medical Research and Treatments
- Microtubule and mitosis dynamics
- Organ Transplantation Techniques and Outcomes
- Cardiac Fibrosis and Remodeling
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant Molecular Biology Research
- Ethics and Legal Issues in Pediatric Healthcare
- Circadian rhythm and melatonin
- Histone Deacetylase Inhibitors Research
- Hepatitis C virus research
- Cancer-related molecular mechanisms research
- Blood groups and transfusion
- Liver Disease and Transplantation
- Reproductive Physiology in Livestock
- Healthcare and Venom Research
- Sperm and Testicular Function
- Acute Myeloid Leukemia Research
- Clinical Nutrition and Gastroenterology
Shanghai East Hospital
2018-2024
Dongyang People's Hospital
2024
Wenzhou Medical University
2024
Harbin Medical University
2024
First Affiliated Hospital of Harbin Medical University
2024
First Affiliated Hospital of Xinjiang Medical University
2005-2023
Xinjiang Medical University
2005-2023
National Health and Family Planning Commission
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
Peking Union Medical College Hospital
2023
Objective: Progestin was recently used as an alternative of gonadotropin-releasing hormone (GnRH) analog for preventing premature luteinizing (LH) surge with the aid vitrification techniques, however, limited data were available about potential progestin in poor responders undergoing vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatment. We performed a randomized parallel controlled trial to investigate difference and GnRH antagonist responders. Methods: A total 340 who...
ObjectiveTo investigate flexibility in starting controlled ovarian stimulation at any phase of the menstrual cycle infertile women undergoing treatment with assisted reproduction.DesignRetrospective cohort study.SettingAcademic tertiary-care medical center.Patient(s)At total 150 patients vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) treatment. Ninety also underwent frozen embryo transfer (FET) procedures.Intervention(s)Depending on when was started, three groups were...
Polycystic ovary syndrome (PCOS) is an endocrine disorder characterized by abnormal hormone levels in peripheral blood and poor-quality oocytes. PCOS a pathophysiological caused chronic inflammation oxidative stress. The aim of this study was to investigate the mechanism melatonin regulation on androgen production antioxidative damage granulosa cells from patients with hypoestrogenia hyperandrogenia. Cumulus-oocyte complexes were collected who had low estrogen follicular fluids. Melatonin...
However, the genetic basis for majority of patients resulting from abnormalities in these phenotypes remains to be elucidated.The cell division cycle 20 (CDC20, HGNC:1723) is coactivator anaphase-promoting complex/cyclosome (APC/ C) during mitosis, and plays a role maintaining genome by regulating spindle assembly checkpoints (Musacchio Hardwick, 2002).In oocytes, activation APC/C CDC20 key step homologue disjunction transition meiosis I II (Jones, 2011).CDC20 therefore an essential...
The use of progestin (P) during ovarian stimulation is effective in blocking the luteinizing hormone (LH) surge women with normal reserve, however, its effects have not been determined poor responders. This study aimed to explore follicular dynamics P-primed minimal A total 204 infertile diminished reserve were allocated into medroxyprogesterone acetate (MPA) group or natural-cycle control an alternating order. MPA (10 mg) was administered daily beginning from early phase and a low dose hMG...
In the present study, we assessed efficiency of four BMSC transplantation methods as a therapy for liver failure. A rat model (80 Sprague-Dawley rats) D-galactosamine (D-gal)/lipopolysaccharide (LPS)-induced acute failure (ALF) was established and rats were divided into 5 groups: hepatic artery injection group, portal vein vena caudalis an intraperitoneal group control (16 per group). Following transplantation, tissue blood samples collected on days 1, 3 7, detected EdU...
Abstract Early embryonic arrest is one of the major causes recurrent assisted reproduction failure. It characterized by delayed development and failure to form viable eight‐cell stage embryos on day 3 an cycle. A recent study reported that biallelic mutations in NLRP5 can cause early arrest. a member subcortical maternal complex, which plays significant role embryogenesis. In this study, we described female consanguineous Chinese family who displayed clinical features identified novel...
Early embryonic arrest denotes premature termination of development in preimplantation embryos, which is one the major phenotypes recurrent assisted reproduction failure. Padi6 proven to be a member subcortical maternal complex (SCMC) mice, essential oocyte maturation and embryogenesis. We other groups previously found that biallelic mutations PADI6 caused female infertility manifesting as early arrest. In this study, we identified two novel homozygous variants (p.Cys163Arg, p. Trp475*)...
Octamer‑binding transcription factor 4 (OCT4) is one of the factors associated with self‑renewal and differentiation in cancer stem cells, crucial for progression various types human malignancy. However, expression function OCT4 pancreatic has not been fully elucidated. The purpose present study was to investigate molecular mechanisms cells. clinical significance assessed by an immunohistochemical assay using a tissue microarray procedure tissues cells different degrees differentiation. A...
Objective. This study is to investigate the effects of bone marrow mesenchymal stem cell (BMSC) transplantation on acute liver failure (ALF). Methods. BMSCs were separated from rat marrow, cultured, and identified by flow cytometry. Rat model with ALF was established injecting D-galactosamine lipopolysaccharide. Rats randomly divided into control group BMSC group. The serum levels alanine aminotransferase (ALT) aspartate (AST) measured at 24 h, 120 168 h after transplantation. Apoptosis...
microRNAs (miRNAs) play critical roles in hepatocellular carcinoma (HCC). However, the expression and biological function of miR‑3653 HCC remain unknown. The present study demonstrated that was significantly decreased tissues cells using qRT‑PCR. A level associated with unfavorable clinical features poor prognosis patients. MTT, BrdU, Transwell western blot assays showed overexpression inhibited growth, migration, invasion epithelial‑mesenchymal transition (EMT) HCCLM3 while its knockdown...
This study aimed to evaluate the cumulative live birth rate (cLBR) of progestin-primed ovarian stimulation (PPOS) protocol versus gonadotropin-releasing hormone antagonist (GnRH-ant) for in vitro fertilization (IVF) cycle infertile women with normal reserve (NOR). Infertile NOR who underwent their first IVF were enrolled an open-label randomized controlled trial. Patients randomly assigned 1:1 receive a freeze-all strategy delayed embryo transfer (PPOS group, n = 174) and fresh (GnRH-ant...
A considerable proportion of autoimmune hemolytic anemia (AIHA) are secondary to underlying disorders, especially syetemic lupus erythematosus (SLE), and the clinical laboratory index for early discrimination between primary SLE-related AIHA has yet be defined. In present study, we proposed novel cytokine patterns in pathogenesis as well parameters timely identification patients.AIHA patients confirmed by immunohematology techniques from September 2010 December 2012 our facility were...
Abstract This study investigated the use of medroxyprogesterone acetate (MPA) or a short protocol for controlled ovarian hyperstimulation (COH) in patients with advanced endometriosis who have normal function, and to compare cycle characteristics pregnancy outcomes after frozen-thawed embryo transfer (FET). was retrospective case-control 244 undering COH. The were allocated three groups: surgery group MPA COH (62 patients, 71 IVF/ICSI cycles, 78 FET cycles); aspiration (85 had “chocolate”...
Endometriosis, the presence of active endometrial tissue outside lining membrane uterine cavity, is a common disease in women childbearing age. The ectopic endometrium has some characteristics tumor tissue, including invasive and migratory abilities. In addition, endometriosis associated with inflammation reduced cellular apoptosis.Western blot analysis, qPCR, immunohistochemistry, immunofluorescence microscopy, Transwell assay, wound healing TUNEL staining.Interleukin-1β (IL-1β) induced...
A receptive endometrium is essential for maternal-embryonic molecular communication during implantation. However, the specific regulatory mechanisms of endometrial capacity remain poorly understood. Here, we examined activating transcription factor 3 (ATF3) expression in human endometria and functional effect ATF3 on embryo attachment vitro. Immunohistochemistry (IHC) was used to assess patterns endometria. Quantitative real-time PCR (qRT-PCR), western blotting immunofluorescence (IF)...
Polycystic ovary syndrome (PCOS) is an endocrinopathy with complex pathophysiology that a common cause of anovulatory infertility in women. Although the disruption circadian rhythms indicated PCOS, role clock etiology these pathologies has yet to be appreciated. The nuclear receptors REV-ERBα and REV-ERBβ are core modulators participate regulation diverse set biological functions. However, expression REV-ERBs their effects remain unclear. Here, we demonstrate levels were lower granulosa...
Oral progestin has recently been used to prevent premature LH surges in ovarian stimulation, and this progestin-primed stimulation (PPOS) is effective safe patients with different reserves. The current data are lacking regarding how individualize the gonadotropin dose regimen for women polycystic syndrome (PCOS). A retrospective cohort trial was performed evaluate efficacy of milder clomiphene citrate (CC) compared standard protocol infertile PCOS. total 220 PCOS were collected classified...
Abstract The potential effects of high basal luteinizing hormone (LH) levels on human reproduction were controversial. To demonstrate the elevated LH outcome patients undergoing in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles, we performed a retrospective data analysis 1011 polycystic ovarian syndrome (PCOS) treated with menopausal gonadotropin and medroxyprogesterone acetate (hMG + MPA) protocol at our center between Nov. 2013 Jun. 2017. PCOS had significantly...
Abstract Context The LH/chorionic gonadotropin receptor (LHCGR) is mainly expressed in gonads and plays important roles estradiol production, ovulation, luteal formation. Women with pathogenic LHCGR variants suffer from infertility, successful fertility treatments for such women have never been reported. Objective purpose of this study was to determine whether can achieve pregnancies through vitro fertilization. Design Three LH resistance infertility their parents underwent exome sequencing....