A5055560098- Reproductive biology and impacts on aquatic species
- Aquaculture Nutrition and Growth
- Fish Ecology and Management Studies
- Sperm and Testicular Function
- Genetic diversity and population structure
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Phylogenetic Studies
- Plant and animal studies
- Epigenetics and DNA Methylation
- Ecology and Vegetation Dynamics Studies
- Coastal wetland ecosystem dynamics
- Insect behavior and control techniques
- Plant and Fungal Species Descriptions
- Aquatic Ecosystems and Phytoplankton Dynamics
- Fish Biology and Ecology Studies
- Insect Pest Control Strategies
- CRISPR and Genetic Engineering
- Insect Utilization and Effects
- Aquaculture disease management and microbiota
- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Intergenerational Family Dynamics and Caregiving
- Chemotherapy-induced cardiotoxicity and mitigation
- Antimicrobial Peptides and Activities
- Biocrusts and Microbial Ecology
Medical College of Wisconsin
2025
South Bohemia research center of aquaculture and biodiversity of hydrocenoses
2020-2024
University of South Bohemia in České Budějovice
2020-2024
Institute of Hydrobiology, Biology Centre, Academy of Sciences of the Czech Republic
2024
Chinese Academy of Sciences
2008-2023
Institute of Geographic Sciences and Natural Resources Research
2023
University of Chinese Academy of Sciences
2016-2023
Hunan Provincial Center for Disease Control and Prevention
2020
Saint Mary's University
2020
Chinese Academy of Fishery Sciences
2020
Abstract Short‐term storage of fish sperm is a simple and cost‐effective process in fishery research from basic to applied perspectives. It has been used elucidate evaluate gamete biology quality, perform vitro fertilization, develop cryopreservation. Sperm short‐term selective breeding, hatchery production advanced molecular investigations such as disease detection commercial species aquaculture well biological conservation threatened or endangered fishes. Several studies have demonstrated...
Abstract Background Over the past few decades, many studies have focused on anthracyclines effect heart (cardiotoxicity), but only a sarcoma. In this study, we harness capabilities of advanced cardiac magnetic resonance imaging (MRI) for characterizing anthracyclines-induced cardiotoxicity in sarcoma and compare results to those from breast cancer patients. Patients methods The patients receive an MRI exam at three timepoints: baseline (pre-treatment), posttreatment, 6-months follow-up....
The purpose of the current study was to analyze phenotypic and functional characteristics common carp (Cyprinus carpio) spermatozoa during in vitro aging investigate whether global DNA methylation is affected by sperm aging. Milt collected from five individual males, stored on ice a refrigerator for up 96 h post stripping (HPS) used fertilize eggs with intervals 1, 24 h. Computer-assisted analysis S3e Cell Sorter employed determine (motility, velocity, concentration viability). In addition,...
Abstract Previous studies have demonstrated that CRISPR/Cas9‐mediated genome editing and transgenesis by integrating vector‐engineered antimicrobial peptide genes (AMGs) are effective to modulate the fish's innate immune system. To generalise knowledge of AMG application in aquaculture, we recruited 544 data entries from a pool empirical studies, which included 23 (two unpublished our team) spanning 12 diseases. We systematically re‐processed re‐analysed these harnessing cross‐disease...
Abstract The biology of fish sperm, an important topic in the research reproduction and fisheries from basic science to evolutionary applied aspects, has implications for aquaculture management, breeding biological conservation. quality spermatozoa plays a vital role fertility, directly affects health performance offspring. Environmental factors that affect determinants usually reflected DNA methylation pattern epigenome, which may change offspring's performance. aim present study was...
Spermatozoa motility kinetics have been found to increase in short-term stored sperm on ice of common carp (Cyprinus carpio) following incubation at a high temperature (20 °C) for 10–20 min. The aim the present study was elucidate contribution ATP store and plasma membrane function temperature-induced sperm. content significantly decreased after 9 days storage ice; however, it remained unchanged incubating higher temperatures. either fresh or 14 were enhanced activation 20 °C. demembranated...
Summary 1. Previously, the Yangtze River connected thousands of shallow lakes which together formed a potamo‐lacustrine system capable sustaining rich variety submerged macrophytes. 2. Potamogeton malaianus is one dominant macrophytes in many this area. Genetic variation and population structure P. populations from ten were assessed using inter‐simple sequence repeat markers. 3. Twelve primer combinations produced total 166 unambiguous bands 117 (70.5%) polymorphic. exhibited moderate level...
The purpose of this study was to investigate the effect an activation solution (AS) (hatchery water (pH 7.9 and 4 mOsm/kg), Perchec (5 mM KCl, 45 NaCl, 30 Tris-HCl; pH 8.0 130 mOsm/kg) Cheng (40 20 Tris; 100 on aging sperm common carp (Cyprinus carpio) after storage in a artificial seminal plasma (carp ASP: 2 CaCl2, 1 MgSO4, 40 110 7.5 310 for 7 days without antibiotics at °C. Fresh milt undiluted were used as control groups. Milt ml) collected diluted 1:1 ASP 50 ml containers stored °C...
Short-term storage of sperm in an extender at a low temperature has been widely applied hatchery management. In the present study, for first time, we examined effects elevating on spermatozoa motility kinetics and fertilization ability after short-term 0–2 °C. Sperm common carp (Cyprinus carpio L.) was diluted with (110 mM NaCl, 40 KCl, 2 CaCl2, 1 MgSO4, 20 Tris, pH 7.5, 310 mOsmol/kg) ratio 1:1 (v/v) containers stored 6–9 days °C under aerobic conditions. At end storage, suspension...
ABSTRACT With the rising global demand for seafood and challenges posed by overfishing climate change, aquaculture sector has become increasingly important in providing high‐quality protein human consumption. Although traditional selection breeding programs have made great strides genetic improvement of species over past decades, faster more precise tools, such as genome manipulation, are needed performance enhancement stock. This review presents a comprehensive overview current status three...
Objectives.Middle-aged adults often provide beneficial support to grown children. Yet, in some relationships, children may feel beholden or intruded upon when they receive parental help. The purpose of this study was examine such conflicted relationships between middle-aged parents and young adults.
Short-term storage of sperm is a useful tool to synchronize the availability male and female gametes for fertilization in hatchery. Induced aging by short-term has been shown affect fish spermatozoa phenotypes including morphology motility kinetics. However, its effects on epigenetics resulting embryos have not investigated. In present study, common carp (Cyprinus carpio) was stored vivo and/or vitro using an extender, kinetics, viability, were analyzed. We observed that velocity decreased...
This is the first report showing fertilization of short-term stored eggs (19 °C) with diluted sperm (0–2 in common carp (Cyprinus carpio). From 5 males, fresh (control) and 7-day were extender. After collection from nine females, immediately pooled into three groups females 1–3, 4–6 7–9, according to eggs' quality based on visual criteria. They placed beakers, covered incubated at 19 °C (for each pool, 10 g female used). The storage was 20 for min before being used fertilization. fertilized...
Satellite radar altimetry has been widely utilized in hydrological research, particularly with the advent of Sentinel-3, a Synthetic Aperture Radar (SAR) altimeter operating globally and equipped an innovative onboard tracking system referred to as open-loop command (OLTC). Utilizing pseudo-DEM (Digital Elevation Model), controlled through OLTC, holds significant promise for reliable observation inland water bodies. Nevertheless, complex geographical conditions high mountain reservoir river...
Abstract Sturgeon spermatozoa are unique for their sustained motility. We investigated the relative importance of bioenergetic pathways in energy supply Siberian sturgeon Acipenser baerii during motile and immotile states. Spermatozoon motility oxygen consumption rate (OCR) were analysed following exposure to inhibitors oxidative phosphorylation (sodium azide, NaN 3 ), glycolysis (2‐deoxy‐D‐glucose, DOG) β‐oxidation fatty acids fluoride, NaF), an uncoupler (carbonyl cyanide m ‐chlorophenyl...
This study was conducted to determine the minimum number of spermatozoa and optimal volume activation water for effective fertilization sterlet Acipenser ruthenus eggs. It also concentration per ml during artificial propagation, when sperm with good motility velocity (95 % 150 μm s−1 curvilinear velocity) were used fertilization. In three experiments, eggs (∼50) from fertilized by pooled four males in replicates incubated at 17 °C. The success evaluated neurulation hatching. Sterlet...