A5069831870- Reproductive biology and impacts on aquatic species
- Sperm and Testicular Function
- Fish Ecology and Management Studies
- Aquaculture Nutrition and Growth
- Glycosylation and Glycoproteins Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive Biology and Fertility
- Gear and Bearing Dynamics Analysis
- Industrial Vision Systems and Defect Detection
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Identification and Quantification in Food
- Viral-associated cancers and disorders
- Galectins and Cancer Biology
- Advanced Proteomics Techniques and Applications
- Systemic Lupus Erythematosus Research
- Pregnancy and preeclampsia studies
- Genetic diversity and population structure
- Lymphoma Diagnosis and Treatment
- Pregnancy and Medication Impact
- Cytomegalovirus and herpesvirus research
- Bone Metabolism and Diseases
- Advanced Decision-Making Techniques
- Bone and Dental Protein Studies
- Viral Infections and Immunology Research
Northwest Women's and Children's Hospital
2024-2025
Shandong Provincial Hospital
2025
Shandong First Medical University
2025
Northwest University
2020-2024
Universiti Malaysia Sarawak
2024
Qilu University of Technology
2024
Yangtze University
2024
Xinjiang Agricultural University
2024
University of South Bohemia in České Budějovice
2017-2022
South Bohemia research center of aquaculture and biodiversity of hydrocenoses
2018-2022
Systemic inflammation is involved in vascular calcification and cardiovascular disease which the leading cause of mortality rheumatoid arthritis (RA). A high level serum interleukin (IL)-6 plays a key role local systemic RA. However, underlying mechanisms remain unclear. We established human umbilical artery smooth muscle cell (HUASMC) culturing method to investigate possible IL-6 on calcification. HUASMCs were obtained from arteries healthy neonates. To detect effects, treated with...
Abstract The quality of frozen/thawed fish sperm is generally lower than that fresh sperm. Extremely low temperatures are associated with damage to spermatozoon subcellular compartments and molecules such as DNA proteins. Cryodamage can negatively affect integrity, metabolism motility, consequently impairing fertilization embryo development. To preserve efficiently, the addition proteins including seminal plasma proteins, antioxidants, antifreeze glycoproteins, bovine serum albumin before...
Spermatozoon represents a very special cell type in human body, and glycosylation plays essential roles its whole life including spermatogenesis, maturation, capacitation, sperm-egg recognition, fertilization. In this study, by mapping the most comprehensive N-glycoproteome of spermatozoa using our recently developed site-specific glycoproteomic approaches, we show that contain number distinctive glycoproteins, which are mainly involved acrosome reaction sperm:oocyte membrane binding, Heavy...
Abstract Background Posterior reversible encephalopathy syndrome (PRES) has been increasingly identified in patients with systemic lupus erythematosus (SLE) owing to the advance neuroimaging techniques. Prompt diagnosis is pivotal improve its outcome. To analyze clinical and radiographic profile of PRES SLE search for appropriate treatment strategy SLE. Methods who fulfilled diagnostic criteria from August 2008 January 2011 were evaluated at baseline, followed determine outcomes. Data...
The purpose of the current study was to analyze phenotypic and functional characteristics common carp (Cyprinus carpio) spermatozoa during in vitro aging investigate whether global DNA methylation is affected by sperm aging. Milt collected from five individual males, stored on ice a refrigerator for up 96 h post stripping (HPS) used fertilize eggs with intervals 1, 24 h. Computer-assisted analysis S3e Cell Sorter employed determine (motility, velocity, concentration viability). In addition,...
Background . Lung cancer is a deadly worldwide, and its pathogenesis treatment methods require continuous research exploration. As representative factor of adaptive immunity, the role interleukin‐17A (IL‐17A) in lung still unclear. The purpose present study was to investigate effect IL‐17A on biological behaviour cells relative mechanism. Methods human adenocarcinoma A549 H1299 cell lines were used for vitro study. effects proliferation, migration invasion assessed by CCK‐8 assay,...
Background Developmental dysplasia of the hip (DDH) is one most common developmental disorders worldwide, caused by a combination genetic and environmental factors. Methods To investigate etiology DDH in proband (a 27-year-old male), we reviewed patient’s clinical data collected peripheral blood samples from his parents. Genomic DNA was extracted, polymerase chain reaction (PCR) amplification performed. Clinical whole-exome sequencing (WES) using next-generation (NGS) conducted to identify...
Tool wear prediction constitutes a critical enabler for intelligent manufacturing systems, providing scientific decision support proactive maintenance scheduling. is challenge in manufacturing, as accurate predictions can optimize production processes, reduce downtime, and lower costs. However, existing methods face significant limitations: traditional machine learning approaches rely heavily on complex feature engineering, requiring extensive domain expertise, which limits their...
The aim of this study was to investigate the correlation between lactate-albumin ratio (LAR) and 28-day mortality in patients with sepsis combined acute kidney failure (SA-AKI). based on eICU database collected data from 1855 SA-AKI. relationship LAR in-hospital assessed using multivariate Cox regression models Kaplan-Meier survival analysis. A generalised summation model also used analyse non-linear mortality. results showed that rate 19.46% (361/1,855), a significant positive (HR: 1.26,...
Abstract Infertility is a global issue, with male factors contributing to approximately 50% of cases. While assisted reproductive technologies (ART) resolve 30%–50% cases, 2%–5% IVF failures require rescue intracytoplasmic sperm injection (RICSI). Sperm glycocalyx critical for capacitation and fertilization, sperm‐derived exosomes, essential cell communication, remain underexplored. This study used lectin microarrays fluorescence histochemistry analyze glycopatterns in exosomes from RICSI...
Introduction The issue of mortality due to sepsis remains a significant concern in the field medicine. Previous researches have demonstrated an association between mean corpuscular volume (MCV) and from range diseases. objective this study was investigate relationship MCV risk large multicentre cohort. Method A retrospective cohort conducted using data eICU Collaborative Research Database 2014–2015. determined within initial 24 hours ICU admission, with patients subsequently classified into...
Epstein–Barr (EB) virus infection has long been speculated to evoke systemic lupus erythematosus (SLE). Since a can induce interferon (IFN) system activation, we aimed discover the relationship between two in progression of SLE Chinese inpatient cohort. Methods Peripheral blood mononuclear cells and sera were isolated from 116 patients 76 healthy controls. Antibodies against EBV-VCA (IgM IgG) EBNA (IgG) along with IFNα patient detected enzyme-linked immunosorbent assays. The EB DNA load was...
Osteoarthritis is the most frequent chronic bone-joint disease in middle-aged and older people worldwide. This study investigated effects of long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) on lipopolysaccharide (LPS)-induced murine chondrogenic ATDC5 cell inflammatory injury. Cell viability apoptosis were assessed using counting kit-8 assay annexin V-phycoerythrin (PE) staining, respectively. The expression levels interleukin-1β (IL)-1β, IL-6, IL-8, tumor...
BACKGROUND B lymphocyte hyperactivity is a main characteristic of systemic lupus erythematosus (SLE), and lymphocytes play prominent pathogenic role in the development progression SLE. The aim this study was to investigate Sirtuin 1 (Sirt1) lymphocytes. MATERIAL AND METHODS Mouse BaF3 transfected with Sirt1 vector or shRNA against Sirt1. Then cells viability apoptosis were respectively determined by MTT assay flow cytometry. In addition, mRNA levels three pro-inflammatory cytokines p53...
The aquaporins (AQPs) are a family of integral membrane proteins involved in the transcellular transport water and other small molecules. A scan apple (Malus domestica) genome revealed presence 42 genes encoding putative AQPs. Based on phylogenetic analysis deduced peptide sequences AQPs generated by Arabidopsis thaliana, poplar (Populus trichocarpa), rubber (Hevea brasiliensis), were each assigned membership five established AQP subfamilies, namely PIPs (eleven members), TIPs (thirteen NIPs...
Lupus nephritis is a frequent and serious complication of systemic lupus erythematosus (SLE). Therefore, better understanding regarding the underlying mechanism renal tubular injury induced by SLE, beneficial to develop different therapeutic strategies for nephritis. The study aimed investigate role miR-130a against lipopolysaccharide-induced glomerular cell injury.HK-2 cells (human proximal tubule cells) were used detecting levels. Cells divided into scramble, miR-130 mimic, siNC,...
Abstract IL-27 could inhibit the development of Th17 cells, and Th17/regulatory T-cell imbalance may reverse maternal tolerance in pre-eclampsia (PE). The aim this study was to investigate association between genetic polymorphisms IL27 with PE. Three SNPs (rs153109, rs17855750, rs181206) were genotyped a Chinese Han cohort 1040 PE patients 1247 normal pregnant women using TaqMan allelic discrimination real-time PCR method. CC genotypic distribution rs153109 significantly higher among cases...