A5041949404- Reproductive biology and impacts on aquatic species
- Sperm and Testicular Function
- Fish Ecology and Management Studies
- Aquaculture Nutrition and Growth
- Fish Biology and Ecology Studies
- Tissue Engineering and Regenerative Medicine
- Reproductive Biology and Fertility
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Erythrocyte Function and Pathophysiology
- Aquaculture disease management and microbiota
- Ovarian function and disorders
- Protein Interaction Studies and Fluorescence Analysis
- Mesenchymal stem cell research
- Aquatic life and conservation
- Nerve injury and regeneration
- Pancreatic function and diabetes
- Lipid Membrane Structure and Behavior
- Pluripotent Stem Cells Research
- Date Palm Research Studies
- Electrospun Nanofibers in Biomedical Applications
- Physiological and biochemical adaptations
- Wound Healing and Treatments
- Marine and fisheries research
- Surfactants and Colloidal Systems
University of South Bohemia in České Budějovice
2018-2024
South Bohemia research center of aquaculture and biodiversity of hydrocenoses
2018-2023
Institute of Hydrobiology, Biology Centre, Academy of Sciences of the Czech Republic
2020
Institute for Problems of Cryobiology and Cryomedicine
2006-2016
National Academy of Sciences of Ukraine
2006-2016
Abstract The lifespan of spermatozoa from externally fertilizing freshwater fish ranges a few seconds to several minutes, depending on the species. External factors, such as temperature, background flows and ion composition, play an important role in fertilization success. Specific mechanisms guiding appear be essential maximize sperm–egg encounter under these strenuous conditions. Although some existing data support hypothesis that both ovarian fluid eggs may release chemoattractants...
Introduction The successful cryopreservation of common carp sperm is crucial for its application in aquaculture and selective breeding programs. This study investigates the efficacy cryopreserving large containers (5 mL) with a low dilution rate (1:1) three different cryoprotective media thawing conditions. Methods developed method utilizes low-ionic (hypotonic) medium, freezing controlled cooling rate, high-temperature (60°C). investigation employs detailed spermatozoon motility assessment....
Abstract We have explored the potential relationship between ploidy level, DNA content (pg nucleus −1 ), and dimensional characteristics, such as volume (μm 3 surface area 2 3‐D structure of erythrocyte nuclei in a series fish level models using Feulgen image analysis densitometry, flow cytometry, confocal laser scanning microscopy. The species were diploid tench ( Tinca tinca ) (2n), Cuban gar Atractosteus tristoechus triploid (3n), evolutionary tetraploid sterlet Acipenser ruthenus (4n),...
Computer assisted sperm analysis (CASA) systems can reduce errors occurring in manual analysis. However, commercial CASA are frequently not applicable at the forefront of challenging research endeavors. The development open source software may offer important solutions for researchers working related areas. Here, we present an example this, with three new modules OpenCASA (hosted Github). first is Chemotactic Sperm Accumulation Module, a powerful tool studying chemotactic behavior, analyzing...
Like seahorses, some of the closely-related pipefish species (Family Syngnathidae) incubate their eggs within a male brood pouch. This has contributed to considerable confusion about sperm transfer mechanisms eggs; authors have reported that ejaculates are released directly into water before they reach eggs, while others suggested fertilised using spermatozoa deposited pouch via an internal duct. Here we present anatomical evidence from freshwater pipefish, Syngnathus abaster, showing not...
The fertilization of freshwater fish occurs in an environment that may negatively affect the gametes; therefore, specific mechanisms triggering encounters gametes would be highly expedient. egg and ovarian fluid are likely major sources these triggers, which we confirmed here for rainbow trout (Oncorhynchus mykiss). affected significantly spermatozoa performance: it supported high velocity a longer period changed motility pattern from tumbling water to straightforward moving fluid. Rainbow...
The review contains the data on scope, properties and localization of resident stem/progenitor cells skin. emphasis is given to populations epithelial dermal stem (SCs), as well melanocyte subcutaneous fat SCs. represent a prospective object for cryobiological technologies establishing low temperature banks use in theoretical experimental biology, general personalized medicine, development bioengineered equivalents tissues artificial organs. Probl Cryobiol Cryomed 2014; 24(1):3–15.
Abstract The primary function of spermatozoa is to fertilize the oocyte, which depends on their motility and directly associated with metabolic state. oxygen consumption rate (OCR) reflects respiratory capacity sperm mitochondria under various physiological conditions an essential marker quality. We determined OCR common carp ( Cyprinus carpio ) using two respirometry methods: conventionally used polarographic method a Clark-type electrode fluorometric assay Oxo Dish optochemical sensor....
Despite available information on the roles of osmotic pressure, potassium (K + ), and calcium (Ca 2+ ) in activation Eurasian burbot spermatozoon motility, changes milt biochemical composition mechanisms underlying their at temperatures above optimal spawning temperature is still unclear. We explored swelling, lipid proteome relation to osmolality swimming medium. The result revealed that increment from 4 30°C increases spermatozoa vulnerability decreasing motility extremely hypotonic media,...
Artificial reproduction of northern pike Esox lucius is impeded by the likelihood obtaining only a small volume sperm inconsistent quality. A controlled-release hormone delivery system has potential to enhance production while avoiding multiple injections The objective this study was investigate effects mammalian gonadotropin-releasing agonist (mGnRHa) incorporated into poly(lactic-co-glycolic acid) (PLGA) microparticles on milt production, spermatozoon characteristics, and secretion...