A5051262543- Reproductive biology and impacts on aquatic species
- Sperm and Testicular Function
- Aquaculture Nutrition and Growth
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Fish Ecology and Management Studies
- Reproductive Biology and Fertility
- Environmental Toxicology and Ecotoxicology
- Membrane Separation and Gas Transport
- Covalent Organic Framework Applications
- Aquaculture disease management and microbiota
- Ovarian function and disorders
- Animal Genetics and Reproduction
- Metal-Organic Frameworks: Synthesis and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Antibiotic Resistance in Bacteria
- Advanced Sensor and Energy Harvesting Materials
- Protein purification and stability
- Fish biology, ecology, and behavior
- biodegradable polymer synthesis and properties
- Bacterial Infections and Vaccines
- Electrospun Nanofibers in Biomedical Applications
- Monoclonal and Polyclonal Antibodies Research
- Membrane Separation Technologies
- Bacterial Genetics and Biotechnology
- Silk-based biomaterials and applications
University of South Bohemia in České Budějovice
2014-2024
South Bohemia research center of aquaculture and biodiversity of hydrocenoses
2014-2024
Charles University
2022-2024
Chinese Academy of Sciences
2018-2019
Qingdao Institute of Bioenergy and Bioprocess Technology
2018-2019
Sewanee: The University of the South
2012-2015
Abstract The quality of frozen/thawed fish sperm is generally lower than that fresh sperm. Extremely low temperatures are associated with damage to spermatozoon subcellular compartments and molecules such as DNA proteins. Cryodamage can negatively affect integrity, metabolism motility, consequently impairing fertilization embryo development. To preserve efficiently, the addition proteins including seminal plasma proteins, antioxidants, antifreeze glycoproteins, bovine serum albumin before...
Oxidant and antioxidant activity in seminal plasma was compared with that spermatozoa of teleost (common carp Cyprinus carpio brook trout Salvelinus fontinalis) chondrostean (Russian sturgeon Acipenser gueldenstaedtii, Siberian baerii, sterlet ruthenus) fishes. No differences were found between the level thiobarbituric-acid-reactive substance (0.24 ± 0.08 to 0.33 0.04 nmol/mg proteins) Russian sturgeon, sterlet. Carbonyl protein concentration significantly higher than all studied species....
We report a simple approach for tailoring the morphology of poly(vinylidene fluoride) (PVDF) membranes fabricated using nonsolvent induced phase separation (NIPS) method that sustains both hydrophilic and hydrophobic properties. Various membrane structures, i.e. skin layers whole structures as well, were obtained via an experimental based on computed ternary diagram. The interactions with polymer solution resulted in different forms properties surface layer affected overall transport solvent...
ABSTRACT The sperm of sterlet ( Acispenser ruthenus ) was used to investigate the effect xenobiotic tetrabrombisphenol A (TBBPA) on quality variables (ATP content, spermatozoa motility, and velocity), DNA integrity, oxidative stress indices. Sperm diluted obtain a density 5 × 10 8 cells/mL exposed for 2 h final concentrations TBBPA (0.5, 1.75, 2.5, 5, μg/L). indices, including lipid peroxidation, carbonyl derivatives proteins, antioxidant activity were significantly higher with increased...
SUMMARY The effect of reactive oxygen species production on the motility parameters, DNA integrity, acid phosphatase activity, and protein tyrosine phosphorylation in spermatozoa common carp ( Cyprinus carpio ) was investigated. Spermatozoa were exposed to different concentrations xanthine oxidase (X‐XO) either presence or absence antioxidants for 15 60 min. A dose‐ time‐dependent reduction velocity observed. Comet assays showed a dramatic increase fragmentation after Changes proteins...
The purpose of the study was to analyze seminal plasma composition, sperm production, and motility over course spawning season in common carp (Cyprinus carpio) rainbow trout (Oncorhynchus mykiss). highest mean percent motile (carp, 98.3 ± 1.6%; trout, 92.8 5.6%) spermatozoon velocity 286.3 7.8 μm sec-1 ; 245.3 8.3 ) were observed middle phase period, at 5 sec post-activation. Sperm volume concentration increased early then decreased significantly toward end both species. Seminal osmolality...
Contents This study investigated the effects of multiple collections sperm on endangered sterlet ( Acipenser ruthenus ) functional parameters [spermatozoa motility and curvilinear velocity (VCL)] as well protein concentration osmolality seminal plasma. The average volume mean spermatozoa per male were significantly altered with collections. On other hand, no significant effect plasma was observed. In all experimental groups, moderate impact sequential collection (p < 0.05) Ninety to 100%...
Spermatozoa of externally fertilizing freshwater fish possess several different modes motility activation. common carp (Cyprinus carpio L.) are activated by hypoosmolality, whereas spermatozoa sterlet (Acipenser ruthenus) require Ca2+ and low concentration K+ for Intracellular signaling differs between these two species as well, particularly in terms utilization secondary messengers (cAMP Ca2+), kinase activities. The current study was performed order to determine the importance protein...