A5077240785- Vector-borne infectious diseases
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Immunotherapy and Immune Responses
- Metal and Thin Film Mechanics
- Vector-Borne Animal Diseases
- Metabolism, Diabetes, and Cancer
- Protein Tyrosine Phosphatases
- Reproductive biology and impacts on aquatic species
- interferon and immune responses
- Toxin Mechanisms and Immunotoxins
- Galectins and Cancer Biology
- Nanoparticles: synthesis and applications
- Antibiotic Resistance in Bacteria
- Antimicrobial agents and applications
- Invertebrate Immune Response Mechanisms
- Pharmaceutical and Antibiotic Environmental Impacts
- Protein Kinase Regulation and GTPase Signaling
- Sperm and Testicular Function
- Growth Hormone and Insulin-like Growth Factors
- Immune Cell Function and Interaction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Polymer Surface Interaction Studies
- Antibiotics Pharmacokinetics and Efficacy
- Plant and Fungal Interactions Research
University of South Bohemia in České Budějovice
2015-2025
Czech Academy of Sciences, Biology Centre
2014-2025
Institute of Parasitology
2011-2025
Czech Academy of Sciences
2011-2012
University of North Carolina at Chapel Hill
2005-2006
Indiana University School of Medicine
2005-2006
International Center for Public Health
2003
Slovak Academy of Sciences
1998-1999
Institute of Virology of the Slovak Academy of Sciences
1998
Macrophage-mediated phagocytosis and cytokine production represent the front lines of resistance to bacterial invaders. A key feature this pro-inflammatory response in mammals is complex remodeling cellular metabolism towards aerobic glycolysis. Although function bactericidal macrophages highly conserved, metabolic insect remains poorly understood. Here, we used adults fruit fly Drosophila melanogaster investigate changes that occur during acute resolution phases Streptococcus-induced...
Type I interferon (IFN), mainly produced by dendritic cells (DCs), is critical in the host defence against tick-transmitted pathogens. Here, we report that salivary cysteine protease inhibitor from hard tick Ixodes scapularis, sialostatin L2, affects IFN-β mediated immune reactions mouse cells. Following IFN receptor ligation, Janus activated kinases/signal transducer and activator of transcription (JAK/STAT) pathway activated. We show L2 attenuates phosphorylation STATs spleen upon addition...
ABSTRACT Th17 cells constitute a subset of CD4 + T lymphocytes that play crucial role in protection against extracellular bacteria and fungi. They are also associated with tissue injury autoimmune inflammatory diseases. Here, we report serpin from the tick Ixodes ricinus , IRS-2, inhibits differentiation by impairment interleukin-6 (IL-6)/STAT-3 signaling pathway. Following activation, mature dendritic produce an array cytokines, including pleiotropic cytokine IL-6, which triggers IL-6 The...
Transmission of pathogens by ticks is greatly supported tick saliva released during feeding. Dendritic cells (DC) act as immunological sentinels and interconnect the innate adaptive immune system. They control polarization response towards Th1 or Th2 phenotype. We investigated whether salivary cystatins from hard Ixodes scapularis, sialostatin L (Sialo L) L2 L2), influence mouse dendritic exposed to Borrelia burgdorferi relevant Toll-like receptor ligands. DCs derived bone-marrow GM-CSF...
Insulin-like growth factor I (IGF-I) stimulates smooth muscle cell (SMC) proliferation, and the mitogen-activated protein kinase (MAPK) pathway plays an important role in mediating IGF-I-induced mitogenic signaling. Our prior studies have shown that recruitment of Src homology 2 domain tyrosine phosphatase (SHP-2) to membrane scaffolding domain-containing substrate-1 (SHPS-1) is required for IGF-I-dependent MAPK activation. The current were undertaken define upstream signaling components are...
Dendritic cells are a sentinel in defending against pathogens and tick saliva facilitates transmission of tick-borne by modulating the host immune response. The maturation dendritic is inhibited saliva. To elucidate mechanism this inhibition, we tested impact Ixodes ricinus on signalling pathways activated Toll-like receptor (TLR-2) ligand Borrelia afzelii spleen cells. activation nuclear factor-κB (NF-κB) p65 phosphatidylinositol-3 kinase (PI3K)/Akt was decreased upon both TLR-2...
Summary Type I interferons (IFN‐α and IFN‐β) are crucial determinants of the host immune response tick saliva modulates this response, thus facilitating transmission tickborne pathogens. The current study therefore examines effect Ixodes ricinus on IFN‐β signalling in murine dendritic cells using lipopolysaccharide (LPS) Borrelia afzelii spirochaetes as inducers. Activated secret IFN that activates Signal Transducer Activator Transcription 1 (STAT‐1). Our results show ‐induced activation...
Abstract Tick cell lines are an easy-to-handle system for the study of viral and bacterial infections other aspects tick cellular processes. cultures often continuously cultivated, as freezing can affect their viability. However, long-term cultivation cells influence genome stability. In present study, we investigated karyotype size lines. Though 16S rDNA sequencing showed similarity between Ixodes spp. at different passages, karyotypes differed from 2n = 28 chromosomes parental ticks, both...
The application of the phagocytic receptor agonists in cancer immunotherapy was studied. Agonists (laminarin, molecules with terminal mannose, N-Formyl-methioninyl-leucyl-phenylalanine) were firmly anchored to tumor cell surface. When particular receptors used together LPS (Toll-like agonist), high synergy causing tumour shrinkage and a temporary or permanent disappearance observed. Methods anchoring (charge interactions, based on hydrophobic chains, covalent bonds) various regimes...
Thin films, which are able to deliver antibiotics in a controlled way, considered as promising approach combat bacterial infections. A novel drug delivery method based on reservoir/diffusion barrier is introduced this paper. As reservoir serves film of sputtered nylon 6,6 impregnated with ampicillin, then covered by diffusion from the same material. It demonstrated that impregnation process does not affect either morphology or surface chemical structure. The amount immobilized can be...
Tick-borne encephalitis virus (TBEV), a member of the genus Flavivirus (Flaviviridae), is causative agent severe neuroinfection. Recently, several flaviviruses have been shown to interact with host protein synthesis. In order determine whether TBEV interacts this process in its natural target cells, we analysed de novo synthesis human cell line derived from cerebellar medulloblastoma (DAOY HTB-186). We observed significant decrease rate synthesis, including housekeeping genes HPRT1 and GAPDH...
The present study aimed to evaluate cryo-injury during the cryopreservation process in sterlet ( Acipenser ruthenus ) sperm, focusing on ultrastructural characteristics. Post-thaw sperm quality parameters, including total motility rate, curvilinear velocity (VCL), linearity (LIN), plasma membrane integrity, antioxidant status, DNA damage, and fine ultrastructure were examined fresh cryopreserved with/without addition of a single optimal dose AFPI (10 μg/mL). A lower VCL increased damage p...