A5084718890- Ubiquitin and proteasome pathways
- Retinal Diseases and Treatments
- Cancer Research and Treatments
- Synthesis and biological activity
- Calpain Protease Function and Regulation
- Cell death mechanisms and regulation
- Retinoids in leukemia and cellular processes
- Endoplasmic Reticulum Stress and Disease
- Phagocytosis and Immune Regulation
- Metal complexes synthesis and properties
- Erythrocyte Function and Pathophysiology
- Cancer, Lipids, and Metabolism
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Lipoproteins and Cardiovascular Health
- Peptidase Inhibition and Analysis
- Retinopathy of Prematurity Studies
- Plant-derived Lignans Synthesis and Bioactivity
- Synthesis and Biological Evaluation
- Protein Degradation and Inhibitors
- Biochemical and Molecular Research
- Computational Drug Discovery Methods
- Blood properties and coagulation
- Autophagy in Disease and Therapy
- Microbial Natural Products and Biosynthesis
Medical University of Warsaw
2016-2025
Charité - Universitätsmedizin Berlin
2005-2006
Photodynamic therapy (PDT) is an approved therapeutic procedure that exerts cytotoxic activity toward tumor cells by inducing production of reactive oxygen species such as singlet oxygen. PDT leads to oxidative damage cellular macromolecules, including proteins undergo multiple modifications fragmentation, cross-linking, and carbonylation result in protein unfolding aggregation. Because the major mechanism for elimination carbonylated their degradation proteasomes, we hypothesized a...
Only a minority of stroke patients receive thrombolytic therapy. Therefore, new therapeutic strategies focusing on neuroprotection are under review, among which, inhibition the proteasome is attractive, as it affects multiple cellular pathways. As inhibitors like bortezomib have severe side effects, we applied novel inhibitor BSc2118, which putatively better tolerated, and analysed its potential in mouse model cerebral ischaemia. Stroke was induced male C57BL/6 mice using intraluminal middle...
Abstract Lovastatin, a drug successfully used in the clinic to prevent and treat coronary heart disease, has recently been reported decrease incidence of melanoma lovastatin‐treated patients. Lovastatin also proved potentiate antitumor effects both cisplatin TNF‐α murine models. Recently, an augmented therapeutic effect lovastatin doxorubicin 3 tumor models mice. In our preliminary study caused retardation growth mice treated with (Feleszko et al. J Natl Cancer Inst 1998;90:247–8). present...
18 (11 novel) Schiff bases, derivatives of salicylaldehyde, 2-hydroxyacetophenone, and 6-acetyl-, 8-acetyl-, 8-formyl-7-hydroxy-4-methylcoumarin were synthesized characterized by their spectral studies. 6-Acetyl-7-hydroxy-4-methylcoumarin was prepared novel method under microwave assistance. These bases evaluated for antibacterial activities against 12 bacterial six fungi strains in vitro. N-(3,5-Dichloro-2-hydroxybenzylidene)-4-aminobenzenesulfonic acid sodium salt proved to be the most...
Homotypic entotic figures, which are a form of “cell-in-cell” structures, considered potential novel independent prognostic marker in various cancers. Nevertheless, the knowledge concerning biological role this phenomenon is still unclear. Since breast cancer cells remarkably entosis-competent, we aimed to investigate and compare frequency entoses primary tumor its lymph node metastasis. Moreover, as there limited data on defined molecular markers entosis, investigated entosis correlation...
Proteasomes play an important role in protein turnover living cells. The inhibition of proteasomes affects cell cycle processes and induces apoptosis. Thus, 20 S proteasomal inhibitors are potential tools for the modulation neoplastic growth. Based on MG132, a potent but nonspecific proteasome inhibitor, we designed synthesized 22 compounds evaluated them proteasomes. majority reduced hydrolysis LLVY-7-aminomethylcoumarin peptide substrate lysates, some drastically. Several displayed...
Abstract Resistance of tumor cells to cisplatin is a common feature frequently encountered during chemotherapy against melanoma caused by various known and unknown mechanisms. To overcome drug resistance toward cisplatin, targeted treatment using alternative agents, such as proteasome inhibitors, has been investigated. This combination could offer new therapeutic approach. Here, we report the biological effects inhibitors on parental cisplatin-sensitive MeWo human cell line its...
Podophyllotoxin (PPT) is an active pharmaceutical ingredient (API) with established antitumor potential. However, due to its systemic toxicity, use restricted topical treatment of anogenital warts. Less toxic PPT derivatives (e.g., etoposide and teniposide) are used intravenously as anticancer agents. has been exploited a scaffold new potential therapeutic agents; however, fewer studies have conducted on the parent molecule than derivatives. We undertaken study ultrastructural changes...
Podophyllotoxin (PPT) is an antimitotic drug used topically in the treatment of anogenital warts. Due to its toxicity it cannot be administered systemically as anticancer agent. However, modified PPT derivatives such etoposide and teniposide are clinically systemic agents. Thus, we invented novel derivative KL3 that was synthesized by photocyclization. Earlier have shown has effect various cell lines. Here compared vs on non-cancerous normal human keratinocytes (HaCaT) peripheral blood...
Inhibition of the proteasome offers many therapeutic possibilities in inflammation as well neoplastic diseases. However, clinical use inhibitors is limited by development resistance or severe side effects. In our study we characterized anti-tumor properties novel inhibitor BSc2118. The sensitivity tumor lines to BSc2118 was analyzed comparison bortezomib using crystal violet staining order assess cell viability. Vivo distribution mouse tissues tracked a fluorescent-modified form (BSc2118-FL)...
A new series of hydroxycoumarin derivatives has been synthesized using conventional synthesis. The syntheses were accelerated by microwave assistance. Yields in both cases comparable (59-69 %). structures established
Inflammatory bowel diseases (IBD) and their main representatives, Crohn’s disease ulcerative colitis, are worldwide health-care problems with constantly increasing frequency still not fully understood pathogenesis. IBD treatment involves drugs such as corticosteroids, derivatives of 5-aminosalicylic acid, thiopurines, others, the goal to achieve maintain remission disease. Nowadays, our knowledge about is continually growing, more specific effective therapies at molecular level wanted. In...
(1): Atopic dermatitis and psoriasis vulgaris are chronic, inflammatory diseases. Clinical presentation usually leads to a proper diagnosis, but sometimes neither clinical examination nor histopathological evaluation can be conclusive. Therefore, we aimed build up novel diagnostic tool check it for accuracy. The main objective of our work was differentiate between healthy skin (C), atopic (AD) (PV) biopsies on the base involucrin (IVL) human β-defensin-2 (hBD-2) concentrations their mRNA, as...