A5068937791- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Lipoproteins and Cardiovascular Health
- Autophagy in Disease and Therapy
- Retinal Diseases and Treatments
- Cancer Research and Treatments
- Genetics and Neurodevelopmental Disorders
- Reproductive Biology and Fertility
- Peptidase Inhibition and Analysis
- NF-κB Signaling Pathways
- Sperm and Testicular Function
- Cancer, Lipids, and Metabolism
- Lipid metabolism and disorders
- Cell death mechanisms and regulation
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Pharmaceutical Economics and Policy
- Microtubule and mitosis dynamics
- Protease and Inhibitor Mechanisms
- Retinoids in leukemia and cellular processes
- Glycosylation and Glycoproteins Research
- Retinopathy of Prematurity Studies
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- Health Systems, Economic Evaluations, Quality of Life
- Cellular transport and secretion
Amgen (United States)
2020-2024
University of Portland
2024
Oregon Health & Science University
2014-2022
FH Foundation
2020
Oregon Medical Research Center
2018-2019
Indiana University School of Medicine
2006-2014
University of Evansville
2006-2014
Indiana University
2006-2012
Day Family Medicine
1999-2010
Deaconess Hospital
2009-2010
We have used RNA interference (RNAi) to examine the functional relationship between valosin-containing protein (VCP/p97/Cdc48p/TER94) ATPase and ubiquitin-proteasome system (UPS) in Drosophila S2 human HeLa cells. In both cell types, RNAi of VCP (and, a lesser extent, certain VCP-interacting proteins) caused significant accumulation high-molecular-weight conjugates ubiquitin, an indication inhibited UPS function. However, decreased levels did not directly inhibit proteasome activity. cells,...
Valosin-containing protein (VCP; p97; cdc48 in yeast) is a hexameric ATPase of the AAA family (ATPases with multiple cellular activities) involved functions, including degradation proteins by ubiquitin (Ub)–proteasome system (UPS). We examined consequences reduction VCP levels after RNA interference (RNAi) VCP. A new stringent method microarray analysis demonstrated that only four transcripts were nonspecifically affected RNAi, whereas ∼30 response to reduced sequence-independent manner....
Photodynamic therapy (PDT) is an approved therapeutic procedure that exerts cytotoxic activity toward tumor cells by inducing production of reactive oxygen species such as singlet oxygen. PDT leads to oxidative damage cellular macromolecules, including proteins undergo multiple modifications fragmentation, cross-linking, and carbonylation result in protein unfolding aggregation. Because the major mechanism for elimination carbonylated their degradation proteasomes, we hypothesized a...
PCSK9i (protein convertase subtilisin/kexin type 9 inhibitors) are set to revolutionize the treatment of hypercholesterolemia in management atherosclerotic risk, but numerous reports have detailed unprecedented barriers access for these drugs. To overcome challenges, our group created a model facilitate provision this new therapy patients who qualify according Food and Drug Administration criteria. This report details real-world follow-up experience use large patient cohort structured ensure...
We have utilized double-stranded RNA interference (RNAi) to examine the effects of reduced expression individual subunits 26 S proteasome in Drosophila S2 cells. RNAi significantly decreased mRNA and protein levels targeted both core 20 PA700 regulatory complex. Cells deficient any several (e.g. dβ5, dRpt1, dRpt2, dRpt5, dRpn2, dRpn12) displayed activity (as judged by hydrolysis succinyl-Leu-Leu-Val-Tyr-aminomethylcoumarin), increased apoptosis, cell proliferation without a specific block...
The purpose of this study was to evaluate the effectiveness a new, fixed, yet individualized dosing regimen activated prothrombin complex concentrate factor VIII inhibitor bypassing activity (FEIBA) for warfarin reversal in setting life-threatening bleeding secondary care center. In report we present retrospective chart review 72 patients who received FEIBA and 69 fresh-frozen plasma (FFP) reverse effects bleeding. cohort, 500 units when initial INR <5 or 1,000 ≥5. administration resulted...
BACKGROUND Sperm aster organization during bovine and human fertilization requires a paternally-derived centriole that must first disengage from the sperm tail connecting-piece. We investigated participation of 26S proteasome in this process. METHODS Proteasome localization enzymatic activity were studied normal pathological spermatozoa by immunocytochemistry enzyme-substrate assays. The role proteasomes zygote development was using pharmacological proteasome-inhibitor, MG132, with...
Homotypic entotic figures, which are a form of “cell-in-cell” structures, considered potential novel independent prognostic marker in various cancers. Nevertheless, the knowledge concerning biological role this phenomenon is still unclear. Since breast cancer cells remarkably entosis-competent, we aimed to investigate and compare frequency entoses primary tumor its lymph node metastasis. Moreover, as there limited data on defined molecular markers entosis, investigated entosis correlation...
Abstract Cu,Zn superoxide dismutase (SOD1) mutations cause one form of familial amyotrophic lateral sclerosis by a toxic gain function that may be related to abnormal protein folding and aggregate formation. However, the processing pathways involved in SOD1 generation within spinal cord remain unclear. We have now developed an experimental system for studying formation clearance intact tissue. Here we demonstrate SOD1‐positive aggregates G93A transgenic mouse tissue involves...
Abstract We have evaluated the effects of three different omega‐3 polyunsaturated fatty acids (ω‐3 PUFA s) – docosahexaenoic acid ( DHA ), eicosapentaenoic EPA ) and docosapentaenoic DPA on fat accumulation expression adipogenic inflammatory markers using both 3T3‐L1 pre‐adipocytes differentiated adipocytes. Our results indicate that ω‐3 s induce degradation synthase through ubiquitin‐proteasome system, which is likely to beneficial metabolic effect adipose cells. Omega‐3 also increase...
In the present study we describe localization of proteasomes in human spermatozoa by means immunolabelling with different monoclonal and polyclonal antibodies detected confocal microscopy. Western blotting confirmed specificity has shown that are seminal fluid. concentrated neck region where centrioles located. Some labelling was also at periphery head, but no proteasomal antigens were either nucleus or associated flagellum. Proteasome inhibitors did not affect motility spermatozoa, acrosome...