A5043054066- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- RNA regulation and disease
- Virus-based gene therapy research
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
Forest Institute
2017-2020
Wake Forest University
2017-2020
Transient expression of the CRISPR/Cas9 machinery will not only reduce risks mutagenesis from off-target activities, but also decrease possible immune response to Cas9 protein. Building on our recent developing a system able package up 100 copies mRNA in each lentivirus-like particle (LVLP) via specific interaction between aptamer and aptamer-binding proteins (ABP), here we develop lentiviral capsid-based bionanoparticle system, which allows efficient packaging Cas9/sgRNA ribonucleoprotein...
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system discovered using bacteria has been repurposed for genome editing in human cells. Transient expression of the editor proteins (e.g. Cas9 protein) is desirable to reduce risk mutagenesis from off-target activity. Using specific interaction between bacteriophage RNA-binding and their RNA aptamers, we developed a able package up 100 copies Staphylococcus aureus (SaCas9) mRNA each lentivirus-like...
The argonaute protein from the thermophilic bacterium Thermus thermophilus shows DNA-guided DNA interfering activity at high temperatures, complicating its application in mammalian cells. A recent work reported that Natronobacterium gregoryi (NgAgo) had genome editing We compared activities of NgAgo and Staphylococcus aureus Cas9 (SaCas9) human HEK293T cells side by side. EGFP reporter assays sequencing consistently revealed SaCas9. However, these did not demonstrate NgAgo. confirmed...