The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system discovered using bacteria has been repurposed for genome editing in human cells. Transient expression of the editor proteins (e.g. Cas9 protein) is desirable to reduce risk mutagenesis from off-target activity. Using specific interaction between bacteriophage RNA-binding and their RNA aptamers, we developed a able package up 100 copies Staphylococcus aureus (SaCas9) mRNA each lentivirus-like...

Background: The potential involvement of type 2 diabetes mellitus (T2DM) as a risk factor for colon cancer (CC) has been previously reported. Epigenetic changes, such deregulation long non-coding RNA (lncRNA) and microRNA (miR), have linked to the advancement CC; however, effects high glucose levels on their and, in turn, remain unexplored. Methods: Fifty patients had dual diagnosis CC T2DM, 60 with without were included study. qRT-PCR was used examine expression lncRNA ANRIL miR-186-5p...

Abstract Disrupted spermatogenesis and testicular injury are among the devastating outcomes of methotrexate. A major contributor to methotrexate-induced testiculopathy is oxidative damage which triggers apoptosis altered autophagy responses. Eicosapentaenoic acid ethyl ester (EPA-E) an antihyperlipidemic derivative omega-3 fatty acids that exhibited affinity peroxisome proliferator-activated receptor-γ (PPAR-γ) possesses both antioxidant modulating properties. This exploratory study aiming...

Obesity is a multifactorial metabolic disease resulting from behavioral and genetic factors. linked to diabetes mellitus hypertension, which are considered as major risk factors for chronic kidney (CKD); moreover, it has direct effect on developing CKD end stage renal (ESRD). Here was aimed examine the association between uncoupling protein 2 (UCP2) gene expression obesity in patients.UCP2 analyzed by real time polymerase chain reaction (RT-PCR) 93 participants divided into three groups. The...

Background: Despite high efficacy-to-toxicity ratio of chemotherapeutic agent, methotrexate, toxicity remains a major concern that limits its use. Inflammatory, oxidative stress and fibrotic pathways are implicated in methotrexate-induced injury. This study aims to investigate the ability losartan limit liver lung injury elucidate possible underlying mechanisms.Methods: Liver was induced adult male Wistar rats via methotrexate ip injection twice weekly for 4weeks (0.5mg/kg), animals were...

Abstract Chronic exposure to heavy metals as aluminum chloride (AlCl 3 ) could result in severe health hazards such chronic renal injury. The present study aimed evaluate the therapeutic potential of adipose tissue-derived stem cells (ASCs) comparison their microvesicles (MV) AlCl -induced Forty-eight adult male Wistar rats were divided into four groups: Control group, -treated /ASC-treated and /MV-treated group. Biochemical studies included estimation serum urea creatinine levels, oxidative...

Objective: The aim of this study was to investigate in vitro and vivo behavior the prepared ethosomal formulations green tea extract, which turn would result enhancing their therapeutic efficacy as antioxidants thermogenic agents obese mice.
 Methods: Fat Wistar rats were acclimated for at least 7 d environmentally controlled cages, then they divided into five groups: 1st received only distilled water, 2nd an oral dose 3rd group extract loaded gel base applied on previously shaven...

Background: Leukemia is a type of cancer arising from white blood cells (WBCs) and resulting malignant transformation different types cell precursors. The objective was to study the DAPK-1 gene methylation pattern in leukemic patients through some light on its possible role as risk factor for leukemia. Methods: Forty-one diagnosed 41 age-matched healthy unrelated volunteers taken control group. analysis aberrant promoter DAPK1 done by specific polymerase chain reaction. Results: results...

Plants hormones are widely used in agriculture to alter plant characteristics as growth regulators. Gibberellic acid (GA3) plays important role promoting growth, and cell differentiation. This work was performed investigate the relationship between lipooxidative damage marker (HEL) bone induced by GA3 adult albino rats. study carried out on 20 rats for 4 weeks treated 6 days/week. The were distributed into 2 groups of 10 each: Groups I (control) II (GA3); each rat oral gavage (75 ppm) once...

Background: Acute myeloid leukemia (AML) is a complex and heterogeneous hematopoietic tissue neoplasm caused by gene mutations, chromosomal rearrangement deregulation of expression. Inositol polyphosphate 4-phosphatase type II (INPP4B) clinically relevant factor in the phosphoinositide 3 kinase (PI3K) pathway- associated cancers, has been found to have bad prognostic role AML. However, exact mechanism still unclear. Objective: We aimed this study investigate INPP4B overexpression Egyptian...

Objectives: To determine the cytokine storm profile in patients with COVID-19 infection relation to disease severity.Methods: Serum levels of TNF-α, IFN-γ, IL-6 and IL-1β were measured by ELISA method IL-1RII, TNFR1 TNFR2 gene expression peripheral blood mononuclear cells (PBMCs) done using real time polymerase chain reaction (PCR) a total 203 patients. The 1st group included 52 severe cases, 2nd comprised 104 moderate cases 47 mild lied 3rd group.Results: There was significant association...

Background: Fat mass and obesity gene (FTO) polymorphism had linked with risks health problems related to in many studies different population. Our study was designed investigate association of FTO rs9939609 COVID-19 severity biochemical parameters such as serum triacylglycerol, cholesterol, transaminases plasma glucose obese Egyptian population.Methods: TaqMan SNP Genotyping Assay (A < T) a sum 253 cases infected COVID-19. Fasting samples insulin, glucose, lipid profile, well ALT AST were...

Increased consumption of Trans Fats is associated with increased risk Coronary Heart Disease. Uncoupling proteins (UCPs) are mitochondrial that disperse the inter-membrane electrochemical potential as heat. We aimed to detect relation high Trans-fat intake in diet on expression UCP2&amp;3 m-RNA children. A specific questionnaire parents sixty-eight children (4-15 years) was conducted. Accordingly, subjects were sub-grouped into; High consuming group (37 subjects) and a Medium one (31...

Abstract Obesity and diabetes prevalence are increasing worldwide. We aimed by this work to detect the possible association of osteoprotegerin (OPG) gene expression with visceral adiposity indices cardiometabolic risk factors among obese patients. This is research enrolled 150 controls cases subdivided into two subgroups non-diabetic (n = 70) 80 patients type 2 mellitus (T2DM). Circulating OPG levels were determined RT-PCR. Serum concentrations assessed ELISA.Our results explored that serum...

Abstract Background: Because of low sensitivity and specificity the currently available urine markers for bladder cancer (BC) detection painful cystoscopy procedure, our study was aimed to evaluate expression urinary exosomal microRNA (miR)-96 miR-183 as probable noninvasive accurate biomarkers diagnosis follow up BC. Methods results: Using quantitative real-time polymerase chain reaction; Expression miR-96 miR- 183 in samples 51 patients with BC, 21 benign lesions 24 normal individuals...