Abstract The Zrt-/Irt-like protein (ZIP) family consists of ubiquitously expressed divalent metal transporters critically involved in maintaining systemic and cellular homeostasis zinc, iron, manganese. Here, we present a study on prokaryotic ZIP from Bordetella bronchiseptica (BbZIP) by combining structural biology, evolutionary covariance, computational modeling, variety biochemical assays to tackle the issue transport mechanism which has not been established for family. apo state...

With the rapid growth of photovoltaic (PV) power in recent years, stability system operation, performance contingency analysis as well quality grid are threatened by inherent uncertainty and fluctuation PV output. It is necessary to have knowledge output characteristics for reliable dispatching. Day-ahead forecasting an effective support achieving optimal Probabilistic can describe that difficult depict deterministic forecasting, results more comprehensive. An ensemble nonparametric...

Abstract Engineering of transporters to alter substrate specificity as desired holds great potential for applications, including metabolic engineering. However, the lack knowledge on molecular mechanisms hinders designing effective strategies transporter Here, we applied an integrated approach rationally preference ZIP8, a Zrt-/Irt-like protein (ZIP) metal with multiple natural substrates, and uncovered determinants specificity. By systematically replacing differentially conserved residues...

Mixed lineage kinase domain–like pseudokinase (MLKL) initiates necroptosis and could serve as a therapeutic target related to series of human diseases. Proteolysis-targeting chimeras (PROTACs) are useful tools for degrading pathological proteins blocking disease processes. Using computer-aided modeling molecular dynamics simulations, we developed covalent MLKL PROTACs by linking optimizing theophylline derivative that covalently targets MLKL. Via structure–activity relationship studies,...

Abstract Development of new multi‐ratiometric fluorescence probes has been a popular research field. This approach provides richer and more accurate information for the recognition different substances which have similar chemical physical properties. In this work, ratiometric sensor UIO‐66⊃FL/RhB is successfully constructed by incorporating fluorescein (FL) rhodamine B (RhB) to UIO‐66. With addition fluoroquinolones (FQs), prepared can be used 2D emission‐fingerprint map, linear discriminant...

The coordinated operation of an integrated energy system (IES) and a distribution network is the inevitable development trend Internet future. day-ahead optimal scheduling IES important way to improve new efficiency economy. When exchange electrical energy, voltage may be out limit. This article presents two-stage joint method for with IESs economy safety network. In first stage, user's demand response interaction between are considered, schedulable potential systems fully tapped. second...

In laminar, fully developed pipe and channel flows that undergo transients from a known initial state, exact analytical solutions for the momentary velocity field as functionals of flow rate can be determined Navier-Stokes equations, arbitrary unsteadiness [Phys. Fluids 12, 518 (2000)]. For duct with uniform wall heating large transients, companion thermal energy equation approximated in form may also solved analytically, yielding instantaneous temperature time both heat flux. Expressions...

The transition route and bifurcations of the buoyant flows developing on a heated horizontal circular surface are elaborated using direct numerical simulations stability analysis. A series bifurcations, as function Rayleigh numbers ( $Ra$ ) ranging from $10^6$ to $6.0\times 10^7$ , found chaos at $Pr=7$ . When $Ra<1.0\times 10^3$ above dominated by conduction, because which distinct thermal boundary layer plume not present. At $Ra=1.1\times 10^6$ Hopf bifurcation occurs, resulting in flow...

The essential microelement zinc is absorbed in the small intestine mainly by transporter ZIP4, a representative member of Zrt/Irt-like protein (ZIP) family. ZIP4 reportedly upregulated many cancers, making it promising oncology drug target. To date, there have been no reports on turnover number which crucial missing piece information needed to better understand transport mechanism. In this work, we used nonradioactive isotope,

Medical implants with customizable design capabilities are an important direction for high-end medical devices. An standard in implant is to match the mechanical properties of implantation site. Inspired by 'cartilage ring-connective tissue' structure natural tracheal, we propose a approach tracheal stent composed 'skeleton + filling matrix'. By establishing mapping model structural parameters and J-type response properties, realize larger degree freedom adjusting properties. This uniquely...

The Daihai Lake, the third largest lake in Inner Mongolia Autonomous Region, is cornerstone to maintain ecosystem balance this region, which facing some problems including size shrinking, water quality declining and biodiversity decreasing largely recent years. In order quantify N purification amount of submerged plants, Stella software was used study construct a nitrogen dynamic model simulate cycle process Lake participation plants process. results showed that based on plant growth area...

Abstract Wind turbine operating conditions are complex. To ensure the turbine’s safe operation, it is essential to carry out condition monitoring and fault diagnosis of its vibration. In this paper, from structure wind turbines, types, formation mechanisms, a vibration system established by designing different sensors combining them with Internet Things technology. The discrete Fourier transform employed preprocess time-frequency data before extracting specific features signal Hilbert-Huang...

Abstract Electrocatalytic conversion of CO 2 to formic acid (HCOOH) represents a promising approach for storing renewable energy and addressing the challenges hydrogen storage transportation. Palladium (Pd) is only known metal capable achieving this process at nearly zero overpotential. However, its practical applications are severely limited by evolution reaction (HER) competition poisoning. Bimetallic alloys, especially intermetallics with ordered structures, offer an effective way...

Lightning invaded over-voltage is the main cause of insulation fault electric apparatus. To acquire actual substation lightning over-voltage, a monitoring system based on arrester valve divider proposed in this paper. The working principle and advantages analyzed firstly. And then, overvoltage waveforms are presented, which recorded by device. At last classified analyzed.

Abstract The essential microelement zinc is absorbed in the small intestine mainly by transporter ZIP4, a representative member of Zrt/Irt-like protein (ZIP) family. ZIP4 reportedly upregulated many cancers, making it promising oncology drug target. To date, there have been no reports on turnover number which crucial missing piece information needed to better understand transport mechanism. In this work, we used non-radioactive isotope, 70 Zn, and inductively coupled plasma mass spectrometry...

Abstract Engineering of transporters to alter substrate specificity as desired holds great potential for applications, including metabolic engineering. However, the lack knowledge on molecular mechanisms hinders designing effective strategies transporter Here, we applied an integrated approach rationally preference ZIP8, a Zrt-/Irt-like protein (ZIP) metal with multiple natural substrates, and uncovered determinants specificity. By systematically replacing differentially conserved residues...