A5103083717- Selenium in Biological Systems
- Bacterial biofilms and quorum sensing
- Moringa oleifera research and applications
- Nanoparticles: synthesis and applications
- Microbial Community Ecology and Physiology
- Pancreatitis Pathology and Treatment
- Seed and Plant Biochemistry
- Animal Nutrition and Physiology
- Aquaculture Nutrition and Growth
- Natural Antidiabetic Agents Studies
- Child Nutrition and Feeding Issues
- Phytochemicals and Antioxidant Activities
- Meat and Animal Product Quality
- Intestinal Malrotation and Obstruction Disorders
- Bacteriophages and microbial interactions
- Enzyme Production and Characterization
- Pancreatic and Hepatic Oncology Research
- Clinical Nutrition and Gastroenterology
- Trace Elements in Health
- Diverse Scientific Research Studies
- Advances in Cucurbitaceae Research
- Essential Oils and Antimicrobial Activity
- Psidium guajava Extracts and Applications
- Intestinal and Peritoneal Adhesions
- Phytochemistry and Bioactivity Studies
Beijing Technology and Business University
2019-2024
Pakistan Council of Scientific & Industrial Research
2019-2024
Karachi Medical and Dental College
2021-2023
University of Swat
2023
International Center for Chemical and Biological Sciences
2023
University of Karachi
2023
University of Peshawar
2021
Kohat University of Science and Technology
2021
Hazara University
2021
Shaheed Mohtarma Benazir Bhutto Medical University
2021
Among the trace elements, selenium (Se) has great demand as a health supplement. Compared to its other forms, nanoparticles have minor toxicity, superior reactivity, and excellent bioavailability. The present study was conducted produce (SeNPs) via biosynthetic approach using probiotic Bacillus subtilis BSN313 in an economical easy manner. exhibited gradual increase Se reduction production of SeNPs up 5–200 µg/mL environmental Se. However, capability decreased beyond that concentration....
In the present study, lysozyme was purified by following multi-step methodology: salt (ammonium sulfate) precipitation, dialysis, and ultrafiltration. The potential measured enzymatic activity after each purification step. However, ultrafiltration, resulting material considered extra purified. It concentrated in an ultrafiltration centrifuge tube, protein/lysozyme used to determine its bactericidal against five bacterial strains, including three gram-positive (
The present study describes the production of biosurfactant from isolate B. licheniformis Ali5. Seven different, previously-reported minimal media were screened for production, and two selected further optimized carbon source. Further, various fermentation conditions such as (pH 2-12, temperature 20-50 °C, agitation speed 100-300 rpm, NaCl (0-30 g·L-1) investigated. partially purified was characterized by Fourier transform infrared spectroscopy (FTIR) matrix-assisted laser...
Objective: The purpose of this study was evaluation the anticancer activity n-hexane and acetone leaf extracts obtained from Delonix regia on BJ, cervical (HeLa), prostate cell lines. Study Design Setting: This in vitro designed to assess cytotoxic effects D. regia. leaves were harvested, dried, then subjected extraction using hexane acetone. resulting concentrated prepared for analysis. these evaluated HeLa lines under standard laboratory conditions. Methodology: MTT colorimetric assay used...
Objective: The purpose of this study was evaluation the anticancer activity n-hexane and acetone leaf extracts obtained from Delonix regia on BJ (normal fibroblast), cervical (HeLa), prostate cell lines. Study Design Setting: This in vitro designed to assess cytotoxic effects D. regia. leaves were harvested, dried, then subjected extraction using hexane acetone. resulting concentrated prepared for analysis. these evaluated HeLa lines under standard laboratory conditions. Methodology: MTT...
Abstract In this study, the transcriptome analysis was practiced to identify potential genes of probiotic Bacillus subtilis BSN313 involved in selenium (Se) enrichment metabolism. The transcriptomic variation strain deliberated presence three different sodium selenite concentrations (0, 3, and 20 μg/mL). samples were taken at 1 13 h subsequent inoculation gene expression profiles Se metabolism analyzed through RNA sequencing. levels pre log phase lower than stationary phase. It is because,...
Lysozyme is a naturally occurring enzyme that ubiquitous and plays an important role in innate immunity through its potent bacteriolytic activity. The main objective of this study was to isolate, screen identify lysozyme-producing Bacillus subtilis strains by 16S rDNA sequencing. Of the four isolated (B18, B66, B70, B124), only one (B66) selected for further because it exhibited comparatively highest enzymatic sequence strain B66 submitted Genbank database with accession number OM327451...
Sudan dyes were investigated in branded and non-branded spices, commonly available the markets of Karachi, Pakistan. High performance liquid chromatography (HPLC) with a variable wavelength detector (VWD) was applied to determine I-IV. The samples had higher concentrations than maximum limits 0.1 mg/kg. highest concentration dye (I) found turmeric powder (8460 mg/kg) lowest (1.50 (IV) Chaat Masala. This indicates that use spices is not safe, whereas no spice samples. Further studies...
The main aim of this work was to screen, isolate, and identify a probiotic selenium (Se)-resistant strain Bacillus subtilis, using the 16S rDNA sequencing approach subsequently optimize conditions. Initially, conditions were enhanced in two univariate optimization environments: shakings flask bioreactor. After solving for selected variables, further optimized orthogonal array testing. results evaluated by analysis variance, support Se enrichment. In bioreactor, based on R F values, order...
Abstract In this study, the effects of temperature on fatty acids profile and degree unsaturation Oreochromis niloticus were investigated. The analysis was performed by gas chromatography. study showed that there large variations (10.0–32.0°C) during period (January–December). highest crude fat content found in January (3380 mg/100 g) lowest June (2050 g). significantly different diversity ( p < 0.05). Total saturated acid (∑SFA) ranged from 409.54 to 1297.61 g, monounsaturated (∑MUFA)...
The present study discusses a biofilm-positive P. aeruginosa isolate that survives at pH levels ranging from 4.0 to 9.0. biofilm consortia were colonized with different phenotypes i.e., planktonic, slow-growing and metabolically inactive small colony variants (SCVs). lower base of the was occupied by SCVs. These cells strongly attached solid surfaces interconnected through network nanotubes. Nanotubes observed stationary phase indwellers more prominent after applying weight consortia....
Abstract Diabetes mellitus is a metabolic disease, characterized by the elevated levels of blood glucose, known as hyperglycemia. It one serious health challenges, and rapidly becoming significant cause morbidity, mortality, worldwide. The persistent hyperglycemic state in diabetes leads to formation advanced glycation end products (AGEs), which identified key factor involved progression diabetes, diabetes‐associated late complications. keeping view medicinal properties indole, thiazole,...
Abstract Wastewater generated by textile industry needs to be treated reduce its toxicity before final disposal and/or for recycling purposes. In the current study, several bacterial strains were screened dye decolorization potential. UV–visible spectroscopy was used determine maximum absorption wavelength of disperse dyes. HPLC and MTS assay confirm degradation detoxification dyes, respectively. Results revealed that indigenously isolated Bacillus licheniformis , Glutamicibacter uratoxydans...
The current study aimed to investigate the control and treatment of biofilm-producing isolates
The increasing demand by consumers for popular healthy fruits, such as the watermelon (Citrullus lanatus), generate substantial bio-wastes, making it imperative to harness value of these by-products. Watermelon has low-calorie content and several other nutritional benefits that make suitable weight management heart-healthy diets. Within this article, composition bioactives which are strongly linked prevention cardiovascular disorders (CVD), cancer, age-related conditions, will be thoroughly...