This research aimed to analyze structural characterization and hypolipidemic activity in vitro of G. lemaneiformis polysaccharide (GLP) its degradation products. The results presented that the content galacturonic acid declined glucuronic level enhanced, average particle size decreased from 99.9 μm 25.7 μm, color brightness strengthened after degraded by H2O2-Vc. There was no significant change thermal stability before degradation. It observed AFM analysis, changed smaller, delicacy...

Porphyra yezoensis has attracted much attention due to its gelling properties and bioactivity. In this study, the chemical structure of polysaccharides (PYPSs) was characterized, effects concentration, temperature, pH, calcium ion (Ca2+) addition on rheological PYPS were systematically investigated. Chemical composition analysis indicated that primarily contained galactose (89.76%) sulfate (15.57%). Rheological tests demonstrated exhibited typical pseudoplastic properties, with apparent...

Gracilaria lemaneiformis is a kind of edible economic red algae, which rich in polysaccharide, phycobiliprotein, pigments, minerals and other nutrients functional components. Polysaccharide one the main active components lemaneiformis, has been reported to present various physiological bioactivities, including regulation glycolipid metabolism, immune, anti-tumor, anti-inflammatory biological activities. This paper aims provide brief summary extraction, purification, structural...

The objective of this research was to investigate the antioxidant activity Gracilarialemaneiformis polysaccharide degradation and its underlying mechanism involved in Nrf-2/Keap-1 signaling pathway HepG2 cells with oxidative stress induced by H2O2. result scavenging ability free radicals showed that GLP-HV (polysaccharide degraded H2O2-vitamin C (Vc)) performed a better than GLP (G.lemaneiformis polysaccharide). Moreover, these from strong weak as follows: superoxide radical, ferric ion,...

Pharmacognosy Magazine,2014,10,40,458-463.DOI:10.4103/0973-1296.141818Published:September 2014Type:Original ArticleAuthors:Bo Qi, Lan Zhang, Zhiqun Jiangqiong Ouyang, and Hui Huang Author(s) affiliations:Bo Qi1, Zhang2, Zhang3, Ouyang1, Huang1 1 Department of Physical Education, Central South University, Changsha, Hunan 410083, China 2 Sports Arts, Zhejiang Yuexiu University Foreign Languages, Shaoxing, 312000, 3 Education Institute, Xinxiang Polytechnic College, Xinxiang, Henan 453000,...

Complex microbial metabolism is key to taste formation in high-quality fish sauce during fermentation. To guide quality supervision and targeted regulation, we analyzed the function of flora fermentation based on a previously developed metagenomic database. The abundance most identified genes involved metabolic functions showed an upward trend In total, 571 proteins extracted from at different stages were identified. These mainly derived Halanaerobium, Psychrobacter, Photobacterium,...

Summary In the present study, influences of different origins and harvest periods on characterisation, structure antioxidant activity Porphyra haitanensis polysaccharides (PHPs) were investigated. PHPR03 obtained from third period (late December) in Raoping possessed highest content sulphate group (14.70%), lowest viscosity (434.34 mL·g −1 ) exhibited better activity. FTIR spectrum confirmed that PHPs had similar functional groups (O—H, C—H C—O group). Thermal gravimetric analysis suggested...

Chinese fermented mandarin fish ( Siniperca chuatsi ) have unique aroma characteristics that are appreciated by local consumers. In this study, electronic nose (E-nose) and gas chromatography–ion mobility spectrometry analyses were combined to establish a volatile fingerprint of during fermentation. Clear separation the data allowed samples at different fermentation stages be distinguishing using E-nose analysis. Forty-three organic compounds identified Additionally, partial least squares...

Abstract Enrofloxacin (ENR) residues in yellow catfish ( Pelteobagrus fulvidraco ) often exceed the standard due to excessive use. This study explored pharmacokinetics of ENR and its metabolite ciprofloxacin (CIP) following a single dose 10 mg/kg body weight via intramuscular injection (IM), oral gavage (PO), or 5‐h drug bath at mg/L 25°C. High‐performance liquid chromatography‐mass spectrometry was used determine CIP concentrations various tissues. The highest concentration occurred with IM...

The influence of harvest time on the photosynthetic protein quality red alga Porphyra dentata was determined using label-free proteomics. Of 2716 differentially abundant proteins that were identified in this study, 478 upregulated and 374 downregulated. top enriched Kyoto Encyclopedia Genes Genomes (KEGG) gene ontology (GO) pathways metabolic processes biosynthetic such as photosynthesis, light harvesting, carbon fixation organisms. Nine important screened. Correlations among their...

Agarose-oligosaccharide production from agar degradation by agarase exhibits lots of advantages and good application prospects. In this study, a novel agar-degrading bacterium Vibrio sp. A8 was isolated red algae in the South China Sea. The whole genome sequencing with comparative genomic secretomic analysis were used to better understand its genetic components about degradation. This strain exhibited artificial seawater after culture optimization. complete (4.88 Mb) comprised two circular...

In the present study, crude polysaccharides were extracted from microalgae Gracilaria lemaneiformis with ultrasonic-assisted extraction method and purified by DEAE-52 cellulose chromatography Sephadex G-100 size-exclusion in that order. Two main fractions, GPC-1and GPC-2, obtained through purification steps. Then partial characterizations, such as chemical composition, ultraviolet (UV) spectrum infrared (IR) of two fractions analyzed this study. The results indicated total sugar contents...

The current study was designed to investigate the anti-fatigue effects of polysaccharide Gynostemma pentaphyllum Makino (GMP). mice were randomly divided into four groups: control group, low-dose GMP-treated middle-dose group and high-dose group. animals received an oral administration physiological saline in a volume 1.0 mL, treated same GMP (50, 100 200 mg/kg bodyweight/day) for 28 days. After days, assessed by forced swimming test some biochemical parameters related fatigue including...

Ultrasonic-freeze-thaw-cycle assisted extraction was used to synchronously extract polysaccharide and phycobiliprotein from Gracilaria lemaneiformis . Firstly, different solvents (deionized water, sodium hydroxide, phosphate buffer solution (PBS) calcium chloride solution) were screened, PBS (1 mM, pH 6.8) considered as the best solvent for its OD 565 value (0.086±0.0003) 595 (0.471±0.002), which significantly higher than any other ( p <0.05). Moreover, an L 9 (3) 4 orthogonal test...

Neuromedin S (NMS), a 36-amino acid neuropeptide, has been found to be involved in the regulation of endocrine activity. It also detected immune tissues mammals, what suggests that NMS may play an important role response. The aim this study was demonstrate presence receptor 1 (NMU1R) and effect pig splenic lymphocytes (SPLs) pulmonary alveolar macrophages (PAMs). NMU1R SPLs PAMs respectively confirmed by reverse transcription-polymerase chain reaction (RT-PCR), western blot analysis...

The conditions for nitrite reductase (NiRs) produced by Lactobacillus plantarum from salted fish were optimized. Enzyme activity of was used as evaluation index. results showed that the pH value had greatest influence on enzyme activity. optimum producing inoculate quantity 4 %, 6.3, culture temperature 35 °C, incubation time 48 h. reached to 89.672 U/mL, which increased 71 % after optimization. This work provided foundation research enzymatic properties reductase. may be useful production products.