A5025105694- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Algal biology and biofuel production
- Lipid metabolism and biosynthesis
- Photosynthetic Processes and Mechanisms
- Birth, Development, and Health
- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- RNA Research and Splicing
- Diet and metabolism studies
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Genetic Syndromes and Imprinting
- Renal and related cancers
- Microbial Community Ecology and Physiology
- Congenital Anomalies and Fetal Surgery
- Mechanisms of cancer metastasis
- Prenatal Screening and Diagnostics
- Sulfur Compounds in Biology
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Developmental Biology and Gene Regulation
- Mesenchymal stem cell research
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2016-2024
Université Grenoble Alpes
2017-2024
CEA Grenoble
2016-2024
Laboratoire Physiologie Cellulaire & Végétale
2003-2024
Centre de recherche Translationnelle en Médecine moléculaire
2020-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022-2024
Centre National pour la Recherche Scientifique et Technique (CNRST)
2016-2024
Centre National de la Recherche Scientifique
2008-2022
Institut de Biosciences et Biotechnologies
2019
Laboratoire de Biologie du Développement
2006-2017
In mammals, totipotent embryos are formed by fusion of highly differentiated gametes. Acquisition totipotency concurs with chromatin remodeling parental genomes, changes in the maternal transcriptome and proteome, zygotic genome activation (ZGA). The inefficiency reprogramming somatic nuclei reproductive cloning suggests that intergenerational inheritance germline contributes to developmental proficiency after natural conception. Here we show Ring1 Rnf2 , components Polycomb-repressive...
Background In the mouse zygote, DNA methylation patterns are heavily modified, and differ between maternal paternal pronucleus. Demethylation of genome has been described as an active replication-independent process, although mechanisms responsible for it remain elusive. Recently, 5-hydroxymethylcytosine suggested intermediate in this demethylation. Methodology/Principal Findings study, we quantified hydroxymethylation both pronuclei zygote during replication period examined their on...
Understanding how marine diatoms adapt to fluctuating environmental conditions is critical for deciphering their ecological success. Here, we investigated the lipidomic responses of model diatom Phaeodactylum tricornutum low temperatures (10°C), darkness, and combined stress. Using a detailed lipidomics approach, revealed significant remodeling plastidial extraplastidial lipid species. Plastidial lipids showed increased unsaturation levels, particularly 16:3-to-16:4 fatty acid switch, only...
Diatoms are unicellular heterokonts, living in oceans and freshwaters, exposed to frequent environmental variations. They have a sophisticated membrane compartmentalization bounded by siliceous cell-wall. Formation of lipid droplets (LDs), filled with triacylglycerol (TAG), is common response stress. The proteome mature-LDs from Phaeodactylum tricornutum highlighted the lack proteins involved early-LD formation, TAG biosynthesis or LD-to-LD connections. These features suggest that cytosolic...
In comparison to in vivo development, how do different conditions of vitro culture ('one step' versus 'sequential medium') impact DNA methylation and hydroxymethylation preimplantation embryos? Using rabbit as a model, we show that are both affected by embryos the effect observed depends on medium used. Correct regulation is essential for embryonic development appears more be key player. Modifications environment early known have long term effects adult phenotypes health; these probably rely...
In Drosophila, PcG complexes provide heritable transcriptional silencing of target genes. Among them, the ESC/E(Z) complex is thought to play a role in initiation whereas other such as PRC1 are maintain it. be recruited DNA through interaction with binding proteins GAGA factor, but no direct interactions between constituents and factor have been reported so far. The Drosophila corto gene interacts E(z) well genes encoding members maintenance complexes, suggesting that it could transition...
BackgroundPolycomb (PcG) and trithorax (trxG) genes encode proteins involved in the maintenance of gene expression patterns, notably Hox genes, throughout development. PcG are required for long-term repression whereas TrxG positive regulators that counteract action. form large complexes bind chromatin at overlapping sites called Polycomb Trithorax Response Elements (PRE/TRE). A third class proteins, so-called "Enhancers Polycomb" (ETP), interacts with either complexes, behaving sometimes as...
Abstract Background Polycomb-group genes ( PcG ) encode proteins that maintain homeotic Hox gene repression throughout development. Conversely, trithorax-group trxG positive factors required for maintenance of long term activation. Both kinds bind chromatin regions called elements (ME). Our previous work has shown corto , which codes a chromodomain protein, and dsp1 an HMGB belong to class the Enhancers trithorax Polycomb ETP interact with both . Moreover, interacts Scr DSP1 protein is...
Diatoms derive from a secondary endosymbiosis event, which occurred when eukaryotic host cell engulfed red alga. This led to the formation of complex plastid enclosed by four membranes: two innermost membranes originating alga chloroplast envelope, and additional peri- epiplastidial (PPM, EpM). The EpM is linked endoplasmic reticulum (ER). most abundant membrane lipid in diatoms monogalactosyldiacylglycerol (MGDG), synthesized galactosyltransferases called MGDG synthases (MGDs), conserved...
Somatic cell nuclear transfer (SCNT) is the injection of a donor nucleus into an enucleated egg. Despite use this technology for many years in research, it still quite inefficient. One causes thought to be incorrect or incomplete genome reprogramming. Embryos produced by (cloned embryos) very often present abnormal epigenetic signatures and irregular chromatin reorganization. Of these two issues, issue rearrangements within nuclei after least studied. It known that cloned embryos...
Mitogen-activated protein kinase (MAPK) cascades are evolutionary conserved transduction pathways involved in many cellular processes. Kinase modules associated with scaffold proteins that regulate signaling by providing critical spatial and temporal specificities. Some of these have been shown to be conserved, both sequence function. In mouse, the MP1 (MEK Partner 1) forms a complex MEK1 ERK1. this work, we focus on Drosophila (dMP1). We show dMP1 is expressed ubiquitously during embryonic...
Biogenesis of photosynthetic membranes depends on galactolipid synthesis, which relies several cell compartments, notably the endoplasmic reticulum (ER) and chloroplast envelope. Galactolipid synthesis involves lipid trafficking between both membrane compartments. In Arabidopsis, ALA10, a phospholipid flippase P4 type-ATPase family, counteracts limitation monogalactosyldiacylglycerol (MGDG) production has positive effect leaf development. ALA10 locates in distinct domains ER depending ALIS...
Abstract Diatoms are one of the largest groups in phytoplankton biodiversity. Understanding their response to nitrogen variations, present from micromolar near‐zero levels oceans and fresh waters, is essential comprehend ecological success. Nitrogen starvation used biotechnological processes, trigger remodeling carbon metabolism direction fatty acids triacylglycerol synthesis. We evaluated whole proteome changes Phaeodactylum tricornutum after 7 days cultivation with 5.5‐mM nitrate (+N) or...
Abstract Diatoms are a major phylum of microalgae, playing crucial ecological roles. They derive from secondary endosymbiosis red alga by an unknown heterotrophic eukaryote, leading to complex intracellular organization. In response unfavorable conditions (stress), diatoms store oil in lipid droplets (LD), raising interest for applications, particular biofuels. spite numerous investigations aiming increase their content, LD biogenesis mechanisms these organisms remain poorly understood. this...
Although the MK3 gene was originally found deleted in some cancers, it is highly expressed others. The relevance of for oncogenesis currently not clear. We recently reported that controls ERK activity via a negative feedback mechanism. This prompted us to investigate potential role cell proliferation. here show overexpression induces proliferative arrest normal diploid human fibroblasts, characterized by enhanced expression replication stress- and senescence-associated markers. Surprisingly,...