A5005501859- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Natural product bioactivities and synthesis
- Phytochemicals and Antioxidant Activities
- Environmental DNA in Biodiversity Studies
- Protist diversity and phylogeny
- Methane Hydrates and Related Phenomena
- Analog and Mixed-Signal Circuit Design
- Digital Filter Design and Implementation
- Plant Virus Research Studies
- Microbial Fuel Cells and Bioremediation
- Tannin, Tannase and Anticancer Activities
- Microbial Metabolic Engineering and Bioproduction
- Plant biochemistry and biosynthesis
- Anaerobic Digestion and Biogas Production
- Phytochemistry and Biological Activities
- Marine and coastal ecosystems
- Enzyme Catalysis and Immobilization
- Biological Activity of Diterpenoids and Biflavonoids
- Evolution and Genetic Dynamics
- Aquatic Ecosystems and Phytoplankton Dynamics
- Chromatography in Natural Products
- Photosynthetic Processes and Mechanisms
- Aquaculture disease management and microbiota
Kyoto University
2016-2025
University of Shiga Prefecture
2025
Kobe University
2001-2023
College of Industrial Technology
2021
Tohoku University
2010-2020
Tokyo University of Science
1997-2017
Graduate School USA
2013-2015
Okayama University
2000-2013
Matsuyama University
2007-2010
Meikai University
2005-2010
ViPTree is a web server provided through GenomeNet to generate viral proteomic trees for classification of viruses based on genome-wide similarities. Users can upload genomes sequenced either by genomics or metagenomics. generates the uploaded together with flexibly selected reference genomes. also serves as platform visually investigate genomic alignments and automatically annotated gene functions genomes, thus providing virus researchers first choice classifying understanding newly...
This paper presents standards and best practices for reporting genome sequences of uncultivated viruses. We present an extension the Minimum Information about any (x) Sequence (MIxS) standard virus genomes. Uncultivated Virus Genome (MIUViG) were developed within Genomic Standards Consortium framework include origin, quality, annotation, taxonomic classification, biogeographic distribution in silico host prediction. Community-wide adoption MIUViG standards, which complement a Single...
Abstract Helicobacter pylori is a major etiological agent in gastroduodenal disorders. In this study, we isolated 36 polyphenols and 4 terpenoids from medicinal plants, investigated their antibacterial activity against H. vitro . All hydrolyzable tannins tested demonstrated promising Monomeric revealed especially strong activity. Other compounds minimal with few exceptions. A monomeric tannin, Tellimagrandin I time‐ dose‐dependent bactericidal On the other hand, did not affect viability of...
The antioxidant polyphenols in cacao liquor, a major ingredient of chocolate and cocoa, have been characterized as flavan-3-ols proanthocyanidin oligomers. In this study, various products were analyzed by normal-phase HPLC, the profiles quantities present, grouped molecular size (monomers~oligomers), compared. Individual polyphenols, (catechin epicatechin), dimeric (procyanidin B2), trimeric C1), tetrameric (cinnamtannin A2) proanthocyanidins, galactopyranosyl-ent-(-)-epicatechin (2α→7,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew Methods of Analyzing TanninsTakuo Okuda, Takashi Yoshida, and Tsutomu HatanoCite this: J. Nat. Prod. 1989, 52, 1, 1–31Publication Date (Print):January 1989Publication History Published online1 July 2004Published inissue 1 January 1989https://pubs.acs.org/doi/10.1021/np50061a001https://doi.org/10.1021/np50061a001research-articleACS PublicationsRequest reuse permissionsArticle Views1220Altmetric-Citations137LEARN ABOUT THESE METRICSArticle Views...
A novel thermophilic, chemoheterotrophic, Gram-negative-staining, multicellular filamentous bacterium, designated strain 110S T , was isolated from an iron-rich coastal hydrothermal field in Japan. The isolate is facultatively aerobic and chemoheterotrophic. Phylogenetic analysis using 16S rRNA gene sequences nested a class-level clone cluster of the phylum ‘ Chloroflexi ’. grows by dissimilatory iron- nitrate-reduction under anaerobic conditions, which first report these abilities organism...
Metagenomics has revealed the existence of numerous uncharacterized viral lineages, which are referred to as "dark matter." However, our knowledge regarding genomes is biased toward culturable viruses. In this study, we analyzed 1,600 (1,352 nonredundant) complete double-stranded DNA (10 211 kb) assembled from 52 marine viromes. Together with 244 previously reported uncultured genomes, a genome-wide comparison delineated 617 genus-level operational taxonomic units (OTUs) for these...
ABSTRACT We isolated a cyanophage (Ma-LMM01) that specifically infects toxic strain of the bloom-forming cyanobacterium Microcystis aeruginosa . Transmission electron microscopy showed virion is composed anisometric head and tail complex consisting central tube contractile sheath with helical symmetry. The morphological features host specificity suggest Ma-LMM01 member cyanomyovirus group. Using semi-one-step growth experiments, latent period burst size were estimated to be 6 12 h 50 120...
ABSTRACT Cyanobacteria and their phages are significant microbial components of the freshwater marine environments. We identified a lytic phage, Ma-LMM01, infecting Microcystis aeruginosa , cyanobacterium that forms toxic blooms on surfaces lakes. Here, we describe first sequenced cyanomyovirus genome Ma-LMM01. The linear, circularly permuted, terminally redundant has 162,109 bp contains 184 predicted protein-coding genes two tRNA genes. exhibits no colinearity with previously genomes...
The abundance of potentially Microcystis aeruginosa-infectious cyanophages in freshwater was studied using g91 real-time PCR. A clear increase cyanophage observed when M. aeruginosa numbers declined, showing that these factors were significantly negatively correlated. Furthermore, our data suggested dynamics may also affect shifts microcystin-producing and non-microcystin-producing populations.
Nanoscale imaging of biological specimens in their native condition is long-standing interest, particular with direct, high resolution views internal structures intact specimens, though as yet progress has been limited. Here we introduce wet coherent x-ray diffraction microscopy capable fully hydrated and unstained specimens. Whole cell morphologies better than 25 nm can be clearly visualized without contrast degradation.
Summary Metagenomics has dramatically expanded the known virosphere, but freshwater viral diversity and their ecological interaction with hosts remain poorly understood. Here, we conducted a metagenomic exploration of planktonic dsDNA prokaryotic viruses by sequencing both virion (<0.22 μm) cellular (0.22–5.0 fractions collected spatiotemporally from deep lake (Lake Biwa, Japan). This simultaneously reconstructed 183 complete (i.e., circular) genomes 57 bacterioplankton...
Sixty-three tannins and related polyphenols were intraperitoneally injected into mice at 4 d before intraperitoneal sarcoma-180 cell inoculation, their antitumor activities evaluated. The condensed compounds all showed negligible activity. As regards the hydrolyzable tannins, active found among ellagitannins. In particular, dimeric ellagitannins such as oenothein B, rugosin E, D, gemin A coriariin significantly higher activity than agrimoniin, which we previously reported an tannin....
Spinocerebellar ataxia type 6 (SCA6) is a neurodegenerative disease caused by the expansion of polyglutamine tract in Ca v 2.1 voltage-gated calcium channel. To elucidate how expanded this plasma membrane protein causes disease, we created unique knockin mouse model that modestly overexpressed mutant transcripts under control an endogenous promoter (MPI-118Q). MPI-118Q mice faithfully recapitulated many features SCA6, including selective Purkinje cell degeneration. Surprisingly, analysis...
Anaerobic Ni-containing carbon-monoxide dehydrogenases (Ni-CODHs) catalyze the reversible conversion between carbon monoxide and dioxide as multi-enzyme complexes responsible for fixation energy conservation in anaerobic microbes. However, few biochemically characterized model enzymes exist, with most Ni-CODHs remaining functionally unknown. Here, we performed phylogenetic structure-based Ni-CODH classification using an expanded dataset comprised of 1942 non-redundant from 1375...
Viruses that infect cyanobacteria are an integral part of aquatic food webs, influencing nutrient cycling and ecosystem health. However, the significance virus host range, replication efficiency, compatibility on cyanobacterial dynamics, growth, toxicity remains poorly understood. In this study, we examined effects cyanophage additions dynamics activity optimal, sub-optimal, non-permissive hosts in cultures Microcystis aeruginosa Raphidiopsis raciborskii. Our findings reveal...
Symbiosis is a basis for species diversification through interactions between organisms. In tropical and subtropical oceans, dinoflagellate symbionts belonging to the family Symbiodiniaceae, including genus Symbiodinium , support flourishment of cnidarian hosts, corals, thereby ecology oligotrophic oceans their photosynthate carbon transfers. Although includes both free-living symbiotic species, detailed genetic background lifestyle differences remains unclear. this study, we identified...