A5089223919- Microbial metabolism and enzyme function
- Liver Disease Diagnosis and Treatment
- Genomics, phytochemicals, and oxidative stress
- Alcohol Consumption and Health Effects
- Hemoglobin structure and function
- Porphyrin Metabolism and Disorders
- Metal-Catalyzed Oxygenation Mechanisms
- Eicosanoids and Hypertension Pharmacology
- Legume Nitrogen Fixing Symbiosis
- Hepatitis C virus research
- Bacterial biofilms and quorum sensing
- HIV/AIDS drug development and treatment
- Metalloenzymes and iron-sulfur proteins
- Enzyme Catalysis and Immobilization
- Adipose Tissue and Metabolism
- Metabolism and Genetic Disorders
- Nitric Oxide and Endothelin Effects
- Cancer, Hypoxia, and Metabolism
- Trace Elements in Health
- Mitochondrial Function and Pathology
- Plant and soil sciences
- Insect symbiosis and bacterial influences
- Heme Oxygenase-1 and Carbon Monoxide
- Biochemical Acid Research Studies
- Microbial Fuel Cells and Bioremediation
Universidad Nacional Autónoma de México
2008-2024
Secretariat of Agriculture, Livestock, Rural Development, Fisheries and Food
2016
Instituto Nacional de Cardiología
2015
ABSTRACT Gluconacetobacter diazotrophicus is an endophyte of sugarcane frequently found in plants grown agricultural areas where nitrogen fertilizer input low. Recent results from this laboratory, using mutant strains G. unable to fix nitrogen, suggested that there are two beneficial effects on growth: one dependent and not fixation. A plant growth-promoting substance, such as indole-3-acetic acid (IAA), known be produced by , could a fixation-independent factor. One strain, MAd10, isolated...
Staphylococcus epidermidis has become a major health hazard. It is necessary to study its metabolism and hopefully uncover therapeutic targets. Cultivating S. at increasing oxygen concentration [O2] enhanced growth, while inhibiting biofilm formation. Respiratory oxidoreductases were differentially expressed, probably prevent reactive species Under aerobiosis, expressed high oxidoreductase activities, including glycerol-3-phosphate dehydrogenase, pyruvate ethanol dehydrogenase succinate as...
Objectives To study the differences in levels of nitrogen metabolites, such as ammonia and nitric oxide correlations existing among them both red blood cells (RBCs) serum, well possible by gender healthy subjects patients with type 2 Diabetes Mellitus (DM). Design methods This cross-sectional included 80 diagnosed DM (40 female 40 male patients) their corresponding controls paired male). We separated serum RBC determined metabolites mainly through colorimetric spectrophotometric assays....
ABSTRACT The characteristics of the respiratory system Acetobacter diazotrophicus PAL5 were investigated. Increasing aeration (from 0.5 to 4.0 liters air min −1 liter medium ) had a strong positive effect on growth and diazotrophic activity cultures. Cells obtained from well-aerated diazotrophically active cultures possessed highly active, membrane-bound electron transport with dehydrogenases for NADH, glucose, acetaldehyde as main donors. Ethanol, succinate, gluconate also oxidized but only...
Several aldehyde dehydrogenase (ALDH) complexes have been purified from the membranes of acetic acid bacteria. The enzyme structures and chemical nature prosthetic groups associated with these enzymes remain a matter debate. We report here on molecular catalytic properties membrane-bound ALDH complex diazotrophic bacterium Gluconacetobacter diazotrophicus. is heterodimer comprising two subunits 79.7 50 kDa, respectively. Reversed-phase high-pressure liquid chromatography (HPLC) electron...
The genus Burkholderia whit over 90 species reported to date, is divided into two major groups phylogenetically distant. first group made up of pathogenic which highlights referred opportunistic pathogens such as cepacia (Bcc) complex; other consists nonpathogenic with skills promote plant growth and rhizoremediation. tropica a bacterium capable fixing nitrogen; isolated from the rhizosphere, rhizoplane, stem roots corn plants sugarcane. Besides its ability diazotrofa, B. presents...
Escherichia coli possesses a two-domain flavohemoglobin, Hmp, implicated in nitric oxide (NO) detoxification. To determine the contribution of each domain Hmp toward NO detoxification, we genetically engineered protein and separately expressed heme (HD) flavin (FD) domains defined hmp mutant. Expression was confirmed by Western blot analysis. CO-difference spectra showed that HD can bind CO, but CO adduct slightly blue-shifted peak. Overexpression resulted an improvement growth to similar...
Gluconacetobacter diazotrophicus is a N2-fixing bacterium endophyte from sugar cane. The oxidation of ethanol to acetic acid this organism takes place in the periplasmic space, and reaction catalyzed by two membrane-bound enzymes complexes: alcohol dehydrogenase (ADH) aldehyde (ALDH). We present strong evidence showing that well-known Alcohol (ADHa) Ga. indeed double function enzyme, which able use primary alcohols (C2-C6) its respective aldehydes as alternate substrates. Moreover, enzyme...
A novel bo 3-type quinol oxidase was highly purified from Bacillus cereus PYM1, a spontaneous mutant unable to synthesize heme and therefore spectroscopically detectable cytochromes aa 3 caa 3. The enzyme contained 12.4 nmol of O 11.5 B mg–1 protein. composed two subunits with an M r 51,000 30,000, respectively. Both were immunoreactive antibodies raised against the oxidase. Moreover, amino-terminal sequence analysis 30-kDa subunit revealed that first 19 residues identical those B. failed...
Utilization of external succinate by Bacillus cereus and the properties purified succinate:menaquinone-7 reductase (SQR) were studied. cells showed a poor ability for uptake respiratory utilization exogenous succinate, thus suggesting that B. lacks specific system. Indeed, genes coding succinate–fumarate transport system missing from genome database cereus. Kinetic studies membranes indicated reduction menaquinone-7 is rate-limiting step in respiration. In accordance with its molecular...
Graphical Abstract Interactions and correlations between metabolites, pancreatic hormones, growth factors. Note the gender differences, frequently opposite among insulin glucagon with factors, as well effect of metabolic disturbances induced by Diabetes type 2. The size arrows indicates strength correlation direction whether was straight or inverse.
Zidovudine (AZT) has significantly reduced the mortality and morbidity rates among AIDS patients, but it been associated with hepatotoxicity. AZT treatment linked to disrupted lipid glucose metabolism, enhancement of pro-inflammatory chemokines other mediators, which can lead liver steatosis. In contrast, administration adenosine (ADO) proven be an effective hepatoprotector against both acute chronic damage. Therefore, present study was aimed investigate harmful effects beneficial...