A5080329735- Erythrocyte Function and Pathophysiology
- Biochemical and Molecular Research
- Pancreatic function and diabetes
- Parasitic Infections and Diagnostics
- Neonatal Health and Biochemistry
- Enzyme Structure and Function
- Endoplasmic Reticulum Stress and Disease
- Trypanosoma species research and implications
- Advanced Glycation End Products research
- Methemoglobinemia and Tumor Lysis Syndrome
- Toxoplasma gondii Research Studies
- Molecular Biology Techniques and Applications
- Antimicrobial Peptides and Activities
- Phagocytosis and Immune Regulation
- Amoebic Infections and Treatments
- Autophagy in Disease and Therapy
- Pneumocystis jirovecii pneumonia detection and treatment
- Cancer, Hypoxia, and Metabolism
- Virus-based gene therapy research
- Cholinesterase and Neurodegenerative Diseases
- Nicotinic Acetylcholine Receptors Study
- Blood properties and coagulation
- Folate and B Vitamins Research
- Enzyme-mediated dye degradation
- Research on Leishmaniasis Studies
Instituto Nacional de Pediatria
2015-2025
Secretaria de Salud
2013-2024
Universidad Nacional Autónoma de México
2011
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common enzyme worldwide, causing a wide spectrum of conditions with severity classified from mildest (Class IV) to severe I). To correlate mutation sites in G6PD resulting phenotypes, we studied four naturally occurring variants: Yucatan, Nashville, Valladolid and Mexico City. For this purpose, developed successful over-expression method that constitutes an easier more precise for obtaining characterizing these enzymes. The kcat...
Hoy en día, los profesionales de la salud que atienden niños saben sus decisiones para prescribir probióticos se basan las propiedades específicas cepa probiótica elijan. La evidencia científica generada durante últimas décadas respalda dicha toma decisiones. Los prebióticos y postbióticos son otros actores importantes están involucrados prescripción productos naturales; sin embargo, estos tienen historias similares a probióticos. inulina achicoria fructanos plantas agave potenciando su uso...
Background/Objectives: Pork tapeworm Taenia solium is the causative agent of cysticercosis which may develop in muscle tissue, skin, eyes, and central nervous system (neurocysticercosis). It estimated by World Health Organization (WHO) that about 2.56–8.30 million are infected worldwide. Praziquantel albendazole used for anthelminthic treatment neurocysticercosis; however, not all patients have a complete elimination cysts, makes it necessary to seek new improved options. Methods: In this...
Abstract Giardiasis, the most prevalent intestinal parasitosis in humans, is caused by Giardia lamblia . Current drug therapies have adverse effects on host, and resistant strains against these drugs been reported, demonstrating an urgent need to design more specific antigiardiasic drugs. ATP production G. depends mainly glycolysis; therefore, all enzymes of this pathway proposed as potential targets. We previously demonstrated that glycolytic enzyme triosephosphate isomerase from (GlTIM),...
Giardiasis is highly prevalent in the developing world, and treatment failures with standard drugs are common. This work deals proposal of omeprazole as a novel antigiardial drug, focusing on giardial glycolytic enzyme used to follow cytotoxic effect at molecular level. We recombinant technology inactivation demonstrate capacity inactivate triosephosphate isomerase, no adverse effects its human counterpart. To establish specific target enzyme, we single mutants every cysteine residue...
Background: The importance of prebiotics consumption is increasing all over the world due to their beneficial effects on health. Production better from endemic plants raises possibilities enhance nutritional in vulnerable population groups. Fructans derived Agave Plant have demonstrated safety and efficacy as animal models. Recently, humans two fructans obtained tequilana (Metlin® Metlos®) was demonstrated. Methods: This study aimed demonstrate Metlin® Metlos® newborns a randomized, double...
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common enzymopathy in world. More than 160 mutations causing disease have been identified, but only 10% of these variants studied at biochemical and biophysical levels. In this study we report on functional structural characterization three naturally occurring corresponding to different classes severity: Class I G6PD Durham, II Santa Maria, III A+. The results showed that Durham (severe deficiency), Maria A+ (less severe...
Deamidation, the loss of ammonium group asparagine and glutamine to form aspartic glutamic acid, is one most commonly occurring post-translational modifications in proteins. Since deamidation rates are encoded protein structure, it has been proposed that they can serve as molecular clocks for timing biological processes such turnover, development aging. Despite importance this process, there a lack detailed structural information explaining effects on structure Here, we studied human...
Research on Giardia lamblia has accumulated large information about its molecular cell biology and infection biology. However, giardiasis is still one of the commonest parasitic diarrheal diseases affecting humans. Additionally, an alarming increase in cases refractory to conventional treatment been reported low prevalence settings. Consequently, efforts directed toward supporting efficient use alternative drugs, study their targets appears promising. Repurposing proton pump inhibitors...
Background We have previously proposed triosephosphate isomerase of Giardia lamblia (GlTIM) as a target for rational drug design against giardiasis, one the most common parasitic infections in humans. Since enzyme exists parasite and host, selective inhibition is major challenge because essential regions that could be considered molecular targets are highly conserved. Previous biochemical evidence showed chemical modification non-conserved non-catalytic cysteine 222 (C222) inactivates...
Gluconacetobacter diazotrophicus is a N2-fixing bacterium endophyte from sugar cane. The oxidation of ethanol to acetic acid this organism takes place in the periplasmic space, and reaction catalyzed by two membrane-bound enzymes complexes: alcohol dehydrogenase (ADH) aldehyde (ALDH). We present strong evidence showing that well-known Alcohol (ADHa) Ga. indeed double function enzyme, which able use primary alcohols (C2-C6) its respective aldehydes as alternate substrates. Moreover, enzyme...
Abstract Human triosephosphate isomerase (HsTIM) is a central glycolytic enzyme and overexpressed in cancer cells with accelerated glycolysis. Triple-negative breast highly dependent on glycolysis typically treated combination of surgery, radiation therapy, chemotherapy. Deamidated HsTIM was recently proposed as druggable target. Although thiol-reactive drugs affect cell growth deamidated HsTIM-complemented cells, the role this protein selective target has not been demonstrated. To delve...
The microsporidia are a large group of intracellular parasites with broad range hosts, including humans. Encephalitozoon intestinalis is the second species most frequently associated gastrointestinal disease in humans, especially immunocompromised or immunosuppressed individuals, children and elderly. prevalence reported worldwide these groups ranges from 0 to 60%. Currently, albendazole commonly used treat microsporidiosis caused by species. However, results treatment variable, relapse can...
Delivery methods during childbirth and their related gut microbiota profiles have important impacts on health later in life, they can contribute to the development of diseases such as obesity, whose highest prevalence rate is found among Mexican child population. Coincidentally, Mexico has one global average annual increase cesarean births (C-section). Since leads world childhood studying relationship between delivery this vulnerable population may be used identify early risk factors for...
Protein glycation impacts enzymatic activity, disrupts metabolic processes, and is associated with chronic degenerative diseases. The human glycolytic enzyme triosephosphate isomerase (HsTIM, TPI1, or HsTPI) various disorders, such as enzymopathies originating from mutations. It also possesses moonlighting functions now recognized a cancer marker. Previous reports of HsTPI mutants have demonstrated significant increase methyl-glyoxal (MGO) due to trioses accumulation. We compared how...
For a better comprehension of the structure-function relationship in proteins it is necessary to identify amino acids that are relevant for measurable protein functions. Because numerous contacts establish within and cooperative nature their interactions, difficult achieve this goal. Thus, study protein-ligand interactions usually focused on local environmental structural differences. Here, using pair triosephosphate isomerase enzymes with extremely high homology from two different...
T-cell acute lymphoblastic leukemia (T-ALL) is a challenging childhood cancer to treat, with limited therapeutic options and high relapse rates. This study explores deamidated triosephosphate isomerase (dTPI) as novel target. We hypothesized that selectively inhibiting dTPI could reduce T-ALL cell viability without affecting normal T lymphocytes. Computational modeling recombinant enzyme assays revealed disulfiram (DS) curcumin (CU) bind inhibit activity the non-deamidated enzyme. At...
Therapeutic strategies for the treatment of any severe disease are based on discovery and validation druggable targets. The human genome encodes only 600–1500 targets small-molecule drugs, but posttranslational modifications lead to a considerably larger proteome. spontaneous conversion asparagine (Asn) residues aspartic acid or isoaspartic is frequent modification in proteins as part process called deamidation. Triosephosphate isomerase (TIM) glycolytic enzyme whose deamidation has been...